RESPONSE OF FIVE KENAF ACCESSIONS TO SHOOT REGENERATION

RULLY D. PURWATI, SUDJINDRO SUDJINDRO, SUDARMADJI SUDARMADJI

Abstract


This expeiment was aimed to study response of fives kenfa accession to shoot regeneration and to establish an appropriate regeneration protocol for kenaf. Theexpeimentwasperformed at Tissue Culture Laboratory Research Institute for Tobacco and Fibre Crops (RITFC), Malang. from June to Octo¬ ber 1997 Cotyledons with plumules attached were used as explanls and culture medium for callus induction was MS-based medium with BAP (2 mg/1) and NAA (0 5 mg/1). Calli produced in this cultures were transferred into MS-based medium containing BAP (2 mg/1) and GA.i (5 mg/1) for shoot initiation. All shoots obtained were then sub-cultured in MS-based medium without regulators (MSO) for root formation. Rooted shoots (plantlets) were acclimatized in the sterile sand and transferred mto sterile soil in the glass house. Results of this experiment showed that the most responsive accessions were Cuba 108/1. followed by KK 60, He 48. PI 324922. and CHN/ 056 H with the average number of shoots per explant 4.32 * 4.21,4.00 + 4.01.3.05 + 2.98,2.80 + 1.47. aid 2.72 + 2.49. respectively, at 40 days ater transfering on shoot regeneration medium Shoots rooted ater 14 days on MSO medium with frequencies of 81 50-93.30%. Healthy plantlets survived and grew well in soil media in the glass house. Therefore, an appropriate shoot regeneration protocol for kenaf was found.

Keywords


Hibiscus cannabinus L;shoots regeneration;tissue culture

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DOI: http://dx.doi.org/10.21082/littri.v5n1.1999.31-37

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