Keragaman Genetik Gandum (Triticum aestivum L) Hasil Persilangan Konvergen

Amin Nur, Karlina Syahruddin, Marcia B. Pabendon

Abstract


Wheat is a subtropical crop with a limited genetic resources in indonesia since it belong to tropical country. Convergent breeding is an effort to expand genetic diversity in wheat and to get the plant genotipes that have spesific characters. The aim of this reserch was to determine  genetic diversity of wheat segregants resulted from convergent breeding using Simple Sequence Repeats (SSRs).  Fifty seven wheat genotipes were tested using 39 SSR markers  to determine their  diversity. The results showed grouping by upgma of similarity matrix  with genetic similarity coefficient ranged from 0.30 to 0.95. The similarity coefficient at 0.3 separated the 57 genotipes into two groups: the first group consisted of segregants, Oasis and Guri1 and Guri2 and the second group consisted of Dewata, Alibey, Rabe/M088, Selayar, HP 1744 and Tepoca/Rabe. Alleles which are formed from the entire genotipes using 39 primers were 137 alleles with a range of base pairs formed between 85.6-553 bp. PIC value and the average number of alleles were 0.35 and 3.51, respectively. The genetic variability being increased in wheat germplasm population and obtained 22 candidates of genotipe recombination having a high value of genetic distance (≥0.75) with a chance of many recombination.

Keywords


Genetic diversity; convergent breeding; simple sequence repeat

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References


Ablett, G., H. Hilland and R. Henry. 2006. Sequence polymorphism discovery in wheat microsatellite flanking regions using pyrophosphate sequencing Molecular Breeding. 17:281-289.

Ali, M.B., M.H.I Amir, B Dirk, H.R Zoran and F Jianming . 2010. Wild tetraploid wheat (Triticum turgidum L,) response to heat stress, Journal of Crop Improvement 4: 228-243p,

Andriani, A, Reflinur and M.B Pabendon. 2015. Genetic Diversity Analysis of Wheat Germplasm Collection of Indonesian Cereals Research Institute using SSR Markers, Sabrao 13th Conggress and International Conference 14-16 September 2015, Bogor.

Botstein, D., R.L. White, M. Skolnickand and R.W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms, American journal of human genetics 32:314,

Briggs, F.N., P.F. Knowles. 1997. Introduction to plant breeding, Reinhold Publishing Corporation, California,

Collard, B.C.Y., M.Z.Z. Jahufer, J.B. Brouwer and E.C.K.Pang. 2005. An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts, Euphytica (2005) 142:169–196.

Griffiths, J.F.A, S.R. Wessler, S.B. Carroll, J. Doebley. 2012. Introduction to genetics analysis 10th edition, W.H. Freemanand Company. New York. Pp832.

Gupta, P.K., R.R. Mir, A Mohan., J Kumar. 2008. Wheat genomics: present status and future prospects. Interna¬tional Journal of Plant Genomics. Pp. 2–36.

Henry, R. 1997. Molecular markers in plant improvement. In: Practical Applications of Plant Molecular Biology. Chapmanand Hall, London. Pp. 99–132.

Huang, X.Q, W. Markus, W Martin, Ganal, S. Orford, Robert, M.D Koebner, and Marion S, Ro ̈der. 2007. Did Modern Plant Breeding Lead to Genetic Erosion in European Winter. Crop Breeding & Genetics. Crop Sci (47) 343–349.

Holland, B. 2006. The Bootstrap, consensus-tree and super-trees, Phylogenetic workshop 16-18 August 2006. South Korea.

Jahufer, M., M. Cooper, J.Ayres and R. Bray. 2002. Identification of research to improve the efficiency of breeding strategies for white clover in Australia: A review. Aust J Agric Res (53) 239–257,

Kumar, S, P. Kumari, U. Kumar, M. Grover, A. K Singh, R. Singh, R.S. Sengar. 2013. Molecular approaches for designing heat tolerant wheat. Review Article J. Plant Biochem. Biotechnol. Spriger.

Liu, K., and S.V. Muse. 2005. Power Marker : an integrated analysis environment for genetik marker analysis. Bioinformatics 21:2128-2129.

Malik, R., R. Tiwari, A. Arora, P. Kumar, S. Sheoran. 2013. Genotypic characterization of elite Indian wheat genotipes using molecular markers and their pedigree analysis. Aust J Crop Sci 7: 561.

Melchinger, A.E, H.F. Utz, C.C. Schön. 2008. Genetic expectations of quantitative trait loci main and interaction effects obtained with the triple test cross design and their relevance for the analysis of heterosis. Genetics (177) 1827–1837.

Melchinger, A.E. 1999. Genetik diversity and heterosis. In: Coors JG, Pandey S, eds. The genetics and exploitation of heterosis in crops. Madison. WI:CSSA. 99–118.

Natawijaya, A. 2012. Analisis genetik dan seleksi generasi awal segregan gandum (Triticum aestivum L,) berdaya hasil tinggi. [Thesis]. Institut Pertanian Bogor (IPB).

Nei, M. and W.H. Li. 1979. Mathematical model for studying genetik variation interms of restriction endonucleases. Proceedings of the National Academy of Sciences 76:5269-5273.

Nur, A. 2014. Perakitan varietas gandum tropis adaptif pada ketinggian ≤400 m dpl potensi hasil ≥ 1,5 t/ha dan pada ketinggian ≥400 m dpl potensi hasil ≥ 4 t/ha. Balai Penelitian Tanaman Serealia, Maros.

Nur, A., K. Syahruddin dan M.J. Mejaya. 2015. Perbaikan genetik gandum tropis toleran suhu tinggi dan permasalahan pengembangannya pada daerah dataran rendah. Jurnal penelitian dan pengembangan pertanian vol (34):1 Bogor.

Pabendon, M.B., M.J. Mejaya, J.Koswara, dan H. Aswidinnoor. 2010. Korelasi jarak genetik berbasis marka mikrosatelit inbrida jagung dengan bobot biji F1. Jurnal Penelitian Pertanian Tanaman Pangan, 29(1).

Rahim, MA, Mia AA, Mahmud F, Zeba N, Arifin K. 2010. Genetic variability, character association and genetic divergence in Mungbean (Vigna radiate L, Wilczek). Plant Omic 3:1-6.

Rajaram, S. 1997. Approach for breeding yield stagnation-CIMMYT’s Perspective, Proceedings of the International Group Meeting on ‘Wheat Research Needs Beyond 2000 AD” Held at Directorate of wheat research, Karnal. India. during 12-14 August 1997.

Rohlf, F. 2002. NTSYS-pc : Numerical taxonomy system version 2.1 Exeter Publishing, Ltd., Setauket, NewYork, USA.

Röder, M.S, V. Korzun, K. Wendehake, J. Plaschke, M.H. Tixier, P. Leroy, M.W. Ganal. 1998. A microsatellite map of wheat. Genetics, 149: 2007-2023.

Salem, K.F.M., A.M. El-Zanaty and R.M. Esmail. 2008. Assessing Wheat (Triticum aestivum L,) Genetik Diversity Using Morphological Characters and Microsatallite Markers. World Journal of Agricultural Sciences 4(5): 538-544.

Siddiq, E. A, L.R. Vemireddy, J. Nagaraju. 2012. Basmati Rices: Genetics, Breeding and Trade, Review, Agric Res 1(1):25–36.

Sneath, P.H.A and R.R. Sokal. 1973. Numerical taxonomy, Freeman, San Francisco. Pp.573.

Syahruddin, K. 2012. Analisis kergaman genetik durian (Durio zibethinus L) menggunakan marka morfologi dan marka molekuler Inter simple sekuens repeat (ISSR). [Thesis] Sekolah pascasarjana, Institut Pertanian Bogor (IPB).

Winter, P, and G, Kahl. 1995. Molecular marker technologies for plant improvement. World Journal of Microbiology & Biotechnology 11:438–448.

Witcombe., J,R, S. Gyawali, M. Subedi, D. S Virk and K. D Joshi. 2013. Plant breeding can be made more efficient by having fewer, better crosses. BMC Plant Biology 13:22.

Yamin, M. 2014. Pendugaan komponen ragam karakter agronomi gandum (Triticum aestivum L,) dan identifikasi marka Simple Sequence Repeat (SSR) terpaut suhu tinggi menggunakan Bulk Segregant analysis (BSA). [Thesis]. Sekolah pascasarjana. Institut Pertanian Bogor (IPB).

Zhang, D., G. Bai, C. Zhu, J. Yu and B.F. Carver. 2010. Genetic diversity, population structure, and linkage disequilibrium in US elite winter wheat. The Plant Genome 3: 117-127.




DOI: http://dx.doi.org/10.21082/jpptp.v1n2.2017.p143-151

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