Study Cekaman Salinitas Terhadap Mutan Padi Pada Kondisi In Vitro

Rossa Yunita, Nurul Khumaida, Didy Sopandie, Ika Mariska


In vitro selection using of salt (NaCl) has been used to select plants that tolerate salinity, so that effective selection process. The purpose of this study was to obtain information about the mechanism of salinity tolerance in rice in vitro conditions. This study consisted of two experiments, they are the selection of salinity tolerance somaclone putative mutants in vitro and study of salinity tolerance mechanism results in in vitro selection. The tolerant plants after being treated NaCl show response to the accumulation of proline is more, the content of K, Mg and Ca which tends to remain and Na content is lower, stomata density is fewer and the length and width ratio stomata is greater.


Putative mutan somaclon; proline; Oryza sativa L

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Ashraf, M., M. R. Foolad. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ. Exp. Bot. 59: 206-216

Bing, L., B. C. Zhao , Y. Z.Shen, Z. J. Huang, R.C. Ge, 2008. Progress of study on salt tolerance and salt tolerant related genes in plant. Journal of Hebei Normal University/ Natural Science Edition. 32(2): 243-248

Cha-um, S., C. Kirdmanee. 2009. Proline accumulation,photosynthetic abilities and growth characters of sugarcane (Saccharum offi cinarum L.) plantlets in response to iso-osmotic salt and water-defi cit stress. Agric. Sci. China 8:51-58.

Chutipaijit, S., S. Cha-um, K. Sompornpailin. 2009. Diferential accumulations of proline and flavonoids in indica rice varieties against salinity. Pak. J. Bot. 41(5): 2497-2506.

Clemente, R. M. P., A. Montoliu, S. I. Zandalinas, C. Ollas, A. G. Cadenas. 2012. Carrizo citrange Plants Do Not Require the Presence of Roots toModulate the Response to Osmotic Stress. The Scientific World Journal. 20(12):1-13

Food and Agricultural Organization (FAO) of United Nations. 2005. Panduan lapang untuk diketahui tentang dampak air laut pada lahan pertanian di Propinsi NAD. FAO. 20 hal.

Greorio, G. B., D. Senadhira, R. D. Mendoza. 1997. Screening rice for salinity tolerance. IRRI. 33p

Hakim, M. A., A. S. Jaraimi, M. Begum, M. M. Hanafi, M. R. Ismail, A. Selamat. 2012 Effect of Salt stress on Germination and early seedling growth of rice (Oryza sativa L). African Journal of Biotechnology 9(13):1911-1918.

Khan, M.A., M.U. Shirazi, Muhammad Ali Khan, S.M. Mujtaba, E. Islam, S. Mumtaz, A. Shereen, R.U. Ansari And M. Yasin Ashraf. 2009. Role of proline, K/Na ratio and chlorophyll content in salt tolerance of wheat (Triticum aestivum l.). Pak. J. Bot 41(2): 633-638.

Martı´nez-Atienza, J., X. Jiang, B. Garciadeblas, I. Mendoza, J. Zhu. J. M. Pardo, F. J. Quintero. 2007. Conservation of the salt overly sensitive pathway in rice. Plant Physiol143(2):1001-1012.

Marschner, P. 2012. Mineral Nutrition of Higher Plants. Third Edition. Elsevier. 649 P.

Matsushita, M., T. Matoh. 2006. Characterization of Na+ exclusion mechanisms of salt-tolerant reed plants in comparison with salt-sensitive rice plants. Hysiologia Plantarum 83(1):170–176.

Shanthi P, Jebaraj S, S Geetha S. 2010. In vitro screening for salt tolerance in Rice (Oryza sativa). Electronic Journal of Plant Breeding. 1(4): 1208-1212.

Shereen, A., R. U. Ansari, S. Yamin, S. Raza, S. Mumtaz, M. A. Khan, S. M. Mujtaba. 2007. Physiological responses of rice (Oryza sativa L.) to saline stress. Pak. J. Bot. 39: 2527-2534.

Song J Y., D. S. Kim, M. C. Lee, J. B. Kim, B. K. Ha, S. J. Yun, S. Y. Karng. 2012. Physiological characterization of gamma-ray salt tolerant rice mutants. AJCS. 6(3):421-429.

Sopandie, D. 2014. Fisiologi adaptasi tanaman terhadap cekaman abiotik pada agroekositem tropika. Bogor. Indonesia.IPB press. 228p.

Taiz, L., E. Zeiger. 2006. Plant Physiology. 4th ed, sinauer Associates, Inc. Publishers, Massachusetts. 320p.

Tavakkoli, E., F. Fatehi, S. Coventry, P. Rengasamy, G. K. McDonald. 2011. Additive effects of Na+ and Cl– ions on barley growth under salinity stress. J. Ex. Bot. 62(6):2189–2203.

Turan, M.A., N. Türkmen, N. Taban. 2007. Effect of NaCl on stomatal resistance and proline, chlorophyll, Na, Cl and K concentrations of lentil plants J. Agron. 6: 378-381

Turan, M.A. , A. H. A. Elkarim, N. Taban, S. Taban. 2009. Effect of salt stress on growth, stomatal resistance, proline and chlorophyll concentrations on maize plant. African Journal of Agricultural Research. 4 (9): 893 – 897.



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