Skrining Salinitas Padi Mutan Insersi Pembawa Activation-Tagging pada Fase Perkecambahan

Anky Zannati, Utut Widyastuti, Satya Nugroho

Abstract


Insert mutation is a method in the functional genomics analysis, using Ac and Ds transposable elements that capable of transposing genes in plant genome, including rice. Using such method, it is possible to identify functional genes, such as abiotic stress tolerance genes in rice plant. The objectives of this research were to screen activated-tag mutant rice for tolerance to salinity stress. Seventy-five lines were chosen from a 1,000 fast screening
experiment to identify mutant responsive to salinity stress. The mutant was validated in three screening batches (A, B and C) at germinating stage in Yoshida solution containing 200g/L NaCl. Three potential tolerant mutants, with the highest vigor index were identified from each batch. Insertion analysis of the nine mutants showed that the activator/dissociation (Ac/Ds) elements were still present in the genome, based on the bar and hpt marker genes as identified from the positive PCR.


Keywords


Rice (Oryza sativa L.); activation-tag; salinity stress



DOI: http://dx.doi.org/10.21082/jpptp.v34n2.2015.p105-111

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Jurnal Penelitian Pertanian Tanaman Pangan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


P-ISSN: 2541-5166
E-ISSN: 2541-5174
Accredited No.148/M/KPT/2020 by Kemenristek/BRIN


Jurnal Penelitian Pertanian Tanaman Pangan has been indexed by:

       


Editorial Office

Jurnal Penelitian Pertanian Tanaman Pangan

Pusat Penelitian dan Pengembangan Tanaman Pangan
Jln Merdeka no. 147, Bogor 16111, Indonesia
Phone/Fax.: +62-251-8312755 
E-mail: publikasi_puslitbangtan@litbang.pertanian.go.id
Website: http://pangan.litbang.pertanian.go.id

View My Stats