Genetic Variability and Heritability of Green Super Rice (GSR) Lines Resistance to Rice Ragged Stunt Virus (RRSV) and Rice Grassy Stunt Virus (RGSV)

Untung Susanto, Nafisah Nafisah, Wage R. Rohaeni, Baehaki S. E., Sarlan Abdul rahman, Jauhar Ali

Abstract


Brown plant hopper (BPH) is one of the main pest of rice in Indonesia and it occurred every years with fluctuate acreage.  BPH is also vector of Rice Ragged Stunt Virus (RRSV) and Rice Grassy Stunt Virus (RGSV).  Green Super Rice (GSR) was designed to have resistance to major pests and diseases so that it need less pesticides and thus save ro environment. GSR was developed in IRRI and China and was tested in Indonesia since 2009 until now (2019).  This research was aimed to study the genetic variability and heritability of 26 selected GSR lines to RRSV and RGSV along with four check varieties.  The experiment was conducted in ICRR Sukamandi and Pusakanagara Experimental Station.  The experiment was arranged following randomized complete block design with three replications.  Transplanting was done into 21 days old seedling into 25 cm x 25 cm planting space of 1 m x 1 m plot.  BPH and virus investation was occured narturally due to BPH outbreak along the season (DS 2010).  Percentage of plant showing RRSV and RGSV symtom was measured as consideration the resistance of plant to the viruses.  The results showed that the tested genotypes had high genetic variability and heritability classified as medium in the resistance to RGSV.  The genotypes showed low genetic variability and heritability in the resistance to RRSV.  It implies that breeder effort is feasible to develop resistant lines to RGSV.  HUANGHUAZHAN and HHZ 12-Y4-Y3-Y1 are consistently resistant to RGSV and can be used as donors in further plant breeding activities. The selection of resistant individuals in populations of plant breeding material recommended to be done on earlier generations for RGSV and further generation for RSSV.


Keywords


genetic variability; heritability; RRSV; RGSV

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References


Allard RW. 1999. Principles of plant breding, 2nd ed. New York.

Baehaki, S.E., M.J. Mejaya. 2014. Wereng cokelat sebagai hama global bernilai ekonomi tinggi dan strategi pengendaliannya. IPTEK Tanaman Pangan 9(1):1-12.

Baehaki, S.E., E.H. Iswanto, D. Munawar, Y.H. Song, I.R. Choi, H.H. Park. 2017. Abilities of Brown Planthopper Immigrant Transmits Rice Ragged Stunt Virus on Rice of Some District of Java-Indonesia. Sch J Agric Vet Sci 4(8):300-310.

Baehaki, SE. 2012. Perubahan pengendalian hama terpadu (PHT) konvensional menuju PHT biointensif. Pros. Sem. Nas. Inovasi Teknologi Berbasis Ketahanan Pangan Berkelanjutan. Puslitbangtan. Buku 2. p. 203-214.

Cabauatan, P. Q., R.C. Cabunagan, I.R. Choi. 2009. Rice viruses transmitted by the brown planthopper Nilaparvata lugens Stål. In. Heong KL, Hardy B (Eds.). 2009. Planthoppers: new threats to the sustainability of intensive rice production systems in Asia. International Rice Research Institute. Los Baños. Philippines. Pp 357-368 IN

Chauchan, B.S., J. Opena, J. Ali. 2015. Renponse of 10 elite ‘Green Super Rice’ genotypes to weed investation in aerobic rice systems. Plant Prod. Sci. 18 (2): 228 – 223.

Dini, A.F.B., I.W. Winasa, S.H. Hidayat. 2015. Identifikasi virus penyebab penyakit kerdil pada tanaman padi di Sukamandi, Jawa Barat. J. Fitopatol Indones. 11(6): 205–210. DOI: 10.14692/jfi.11.6.205.

Du, P.V., Cabunagan RC, Choi I-R. 2005. Rice “yellowing syndrome” in the Mekong River delta. Omonrice 13:136-139.

Guan, Y.S., R. Serraj, S.H. Liu, J.L. Xu, J. Ali, W.S. Wang, E. Venus, L.H.Zhu, and Z.K. Li. 2010. Simultaneously improving yield under drought stress and non stress condition: a cases study of rice (Oryza sativa L.). Journal of Experimental Botany 61(15): 4145 – 4156.

Hibino H, Cabauatan PQ, Omura T, Tsuchizaki T. 1985. Rice grassy stunt virus strain causing tungro-like symptoms in the Philippines. Plant Dis. 69:538-541.

Ishak. 2012. Sifat Agronomis, Heritabilitas dan Interaksi G x E Galur Mutan Padi Gogo (Oryza sativa L.). J. Agron. Indonesia 40 (2) : 105 – 111

Iswanto, H.E. 2015. Genetic variation and heritability of resistances rice to ragged stunt and grassy stunt virus. AGRIS. http://agris.fao.org/agris-search/search.do?recordID=ID2015010128

Khush GS, Ling KC. 1974. Inheritance of resistance to grassy stunt virus and its vector in rice. J. Hered. 65:134-136

Le, T.T., Luong, V. D., Ngo, T.T.N., Pham, V.K. 2012. Induced systemic resistance against rice grassy stunt virus – a promising field for ecological rice production. J. Viet. Env. 2012, Vol. 2, No. 1: 48-53

Ling, K.C., E.R. Tiongco, V.M. Aguiero. 1978. Rice ragged stunt, a new virus disease. Plant Dis Rep. 62:701–705

Mc. Whiter, K.S. 1979. Breeding of cross pollinated crops.in R.Knight (ed) plant breeding. Australian vice consellors committe. Brisbane.

Nguyen TD, Lacombe S, Bangratz M, Ta HA, Vinh do N, Gantet P, Brugidou C. 2015. P2 of Rice grassy stunt virus (RGSV) and p6 and p9 of Rice ragged stunt virus (RRSV) isolates from Vietnam exert suppressor activity on the RNA silencing pathway. Virus Genes. 51(2):267-75. doi: 10.1007/s11262-015-1229-2.

Nuque, F.L, Aguiero, V.M., and Ou, S.H. 1982. Inheritance of resistance to grassy rtunt virus in rice. Plant Disease 66:63-64.

Pinaria, A., A. Baihari, R.Setimihardja, dan A.A. Dradjat 1995. Variabilitas genetik dan heritabilitas karakter karakter biomasa 53 genotipe kedelai. Zuriat 6 (2) : 88-92.

Rohmah, N.A., U. Susanto, and T. S. Kadir. 2013. Resistance of Green Super Rice (GSR) Lines to Bacterial Leaf Blight. Proceeding International Seminar. Technology Innovation for Increasing Rice Production and Conserving Environment Under Global Climate Change. ISBN : 9/8-9/9-540-0/2-1. IRRI - Indonesian Center for Rice Research. Indonesia Agency for Agricultural Research and Development Ministry of Agriculture Republic of Indonesia.

Sabu, K.K., M.Z. Abdullah, L.S Lim, R. Wickneswari. 2009. Analysis of heritability and genetic variability of agronomically important tarits in Oryza sativa L. x O. rufipogon. Cross. Agronomy Res. 7:97-102.

Satoh, K., K. Yoneyama, H. Kondoh, T. Shimizu, T. Sasaya, Ry. Choi, K. Yoneyama, T. Omura, Sh. Kikuchi. 2013. Relationship between gene responses and symptoms induced by Rice grassy stunt virus. Front. Microbiol. 4: 1-10.

Shimizu, T, T. Ogamino, A. Hiraguri, E. Nakazono-Nagaoka, T. Uehara-Ichiki, M. Nakajima, K. Akutsu, T. Omura, and T. Sasaya. 2013. Strong Resistance Against Rice grassy stunt virus Is Induced in Transgenic Rice Plants Expressing Double-Stranded RNA of the Viral Genes for Nucleocapsid or Movement Proteins as Targets for RNA Interference. The American Phytopathological Society. Vol. 103, No. 5: 513 – 519.

Singh, R.K. and B.D. Chaudhary. 1979. Biometrical methods in quantitative genetics analysis. Kalyani Publisher. New Delhi.

Setyorini, S.D., Shoahuddin, A. Sulistyo. 2013. Existence of brown planthopper’s natural enemies on some rice varieties using different cultivation techniques. J ournal of Agronomy Research 2(5):8-17.

Suprihanto, S. Somowiyarjo, S. Hartono, Y.A. Trisyono. 2015. Identification and molecular diversity of rice ragged stunt virus and rice grassy stunt virus in java, Indonesia. International Journal of Sciences: Basic and Applied Research. 24(5): 374-386.

Suprihanto, S. Somowiyarjo, S. Hartono, dan Y.A. Trisyono. 2016. Preferensi wereng batang cokelat terhadap varietas padi dan ketahanan varietas padi terhadap virus kerdil hampa. Penelitian Pertanian Tanaman Pangan 35(1): 1-8.

Susanto, U. and Jumali. 2011. Karakteristik Mutu Tanak Galur-galur Green Super Rice (GSR) Terseleksi di Indonesia. In : T. Agung, Suwarto, N.D. Sasongko, A.H. Susanto, Tj. Winanto, dan A. Riyanto (Eds). Proceeding Seminar Nasional Pemuliaan Berbasis Potensi dan Kearifan Lokal Menghadapi Tantangan Globalisasi. PERIPI Komda Banyumas LPPM Univesitas Jenderal Soedirman, ISBN 9786021923900.

Sutrisno. 2015. Resistensi wereng batang cokelat padi, Nilaparvata lugens stål terhadap insektisida di Indonesia. Jurnal AgroBiogen 10(3):115-124.

Ta HA, Nguyen DP, Causse S, Nguyen TD, Ngo VV, Hébrard E. 2013. Molecular diversity of Rice grassy stunt virus in Vietnam. Virus Genes. 46(2):383-6. doi: 10.1007/s11262-012-0845-3

Wang, Z.,L. Yu, L. Jin, W. Wang,Q. Zhao, L. Ran, X. Li, Z. Chen, R. Guo, Y. Wei, Z. Yang, E. Liu, D. Hu, B. Song. 2017. Evaluation of rice resistance to southern rice black-streaked dwarf virus and rice ragged stunt virus through combined field tests, quantitative real-time pcr, and proteome analysis. Viruses 9(2): 37. doi: 10.3390/v9020037.

Wu., J., . Du, C. Wang, L. Cai, M. Hu, Q. Lin, Z. Wu, Y. Li, L. Xie. 2010. Identification of Pns6, a putative movement protein of RRSV, as a silencing suppressor. Virology Journal (7):335 – 740.

Yaqoob, M., R.A. Mann, S.M. Iqbal, and M. Anwar. 2011. Reaction of rice genotypes to brown spot disease pathogen Cochliobolus miyabeanus under drought condition. Mycopath 9 (1): 9 – 11.

Zhang, Q, 2007. Strategies for developing green super rice, PNAS, 104:43, pp 16402-16409.




DOI: http://dx.doi.org/10.21082/jpptp.v3n3.2019.p111-116

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