Isolasi dan Identifikasi Ras Pyricularia grisea pada Tanaman Padi Asal Manokwari dan Manokwari Selatan, Papua Barat

Santoso Santoso, Surianto Sipi, Subiadi Subiadi, Anggiani Nasution

Abstract


Manokwari and South Manokwari Regency is one of the rice development areas in West Papua. Planting of lowland rice in Manokwari and South Manokwari always infect by blast disease. The use of resistant varieties is a major component in controlling blast disease and is the most effective, economical, and easy way for farmers. The use of blast resistant varieties is limited by space and time; in this case the use of resistant varieties must be adjusted to the composition of the P. grisea races in the area. Until now there has been no isolation and identification of P. grisea races that developed in Manokwari and South Manokwari Regency. The study aimed to identify the composition of P. grisea races in Manokwari and South Manokwari areas. The research was carried out in the laboratory and greenhouse of the Muara Bogor Experimental. Isolation and identification of race P. grisea was carried out in 2017. The results showed that the results of P. grisea isolation using monoconidia obtained 34 isolates, which were obtained from the Prafi, Masni, Oransbari and Sidey Districts. Race identification of P. grisea was obtained by 9 race groups namely race 211, 213, 241, 251, 253, 313, 333, 353 and race 373, which were dominated by race 333 as much as 41.18% and race 373 by 33.35%. The P. grisea races obtained are races that have a high virulence level.


Keywords


identification; monoconidia; race of P. grisea; blast disease

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References


Bustamam, M., Reflinur, D. Agisimanto dan Suyono. 2004. Variasi genetic padi tahan blas berdasarkan sidik jari DNA dengan markah gen analog resisten. Jurnal Bioteknologi Pertanian. 9(2): 56-61.

Fang, X., P. Snell, M. J. Barbetti and V. Lanoiselet. 2016. Rice varieties with resistance to multiple races of Magnaporthe oryzae offer opportunities to manage rice blast in Australia. Annals of Applied Biology. 170(2): 160-169.

Ghatak, A., L. Willocquet, S. Savary and J. Kumar. 2013. Variability in aggressiveness of rice blast (Magnaporthe oryzae) isolates originating from rice leaves and necks: a case of pathogen specialization?. Plos One. 8(6): 1-7.

Indrayani, S., A. Nasution dan E. S. Mulyaningsih. 2013. Analisis ketahanan padi gogo dan padi sawah (Oryza sativa L) terhadap empat ras penyakit blas (Pyricularia grisea Sacc). Jurnal Agricola. 3(1): 53-62.

IRRI. 2014. Standard evaluation system for rice. 5th eds. IRRI. Los Banos, Phillippines.

Lestari, A., U. Widyastuti, W. Enggarini. 2016. Uji virulensi 100 isolat blas (Pyricularia oryzae Cavara) terhadap satu set varietas padi diferensial di Indonesia. Jurnal Agrotek Indonesia. 1(1):37-46.

Liu, Y., X. Qi, N. D. Young, K. M. Olsen, A. L. Caicedo and Y. Jia. 2015. Characterization of resistance gene to rice blast fungus Magnaporthe oryzae in a “Green Revolution” rice variety. Molecular Breeding. 35(52): 1-8.

Mogi, S., Z. Sugandhi, S. W. Baskoro, R. Edwina and I. Cahyadi.1991.Establishment of the Differential Variety Series for Pathogenic Race Identification of Rice Blast fungus and the Distribution of Race Based on the New Differential Indonesia. Rice Disease Study Grup Karawang. Jatisari. Indonesia. 30p.

Mulyaningsih, E. S., A. Y. Perdani, S. Indrayani dan Suwarno. 2016. Seleksi fenotipe populasi padi gogo untuk hasil tinggi, toleran aluminium dan tahan blas pada tanah masam. Jurnal Penelitian Pertanian Tanaman Pangan. 35(3): 191-197.

Namai, T. 2011. Race differentiation of the rice blast fungus, Pyricularia oryzae, and environmentally friendly control of rice blast disease. J. Gen Plant Pathol. 77:350–353.

Prabawa, P. S., I. Yulianah dan N. Basuki. 2015. Uji ketahanan 10 genotipe padi merah (Oryza sativa L.) terhadap penyakit blas daun (Pyricularia oryzae Cav.) ras 173. Jurnal Produksi Tanaman. 3(6): 496-502.

Rahim, H. A., M. A. R. Bhuiyan, A. Saad, M. Azhar and R. Wickneswari. 2013. Identification of virulent pathotypes causing rice blas disease (Magnaporthe oryzae) and study on single nuclear gene inheritance of blast resistance in F2 Population derived from Pongsu Seribu 2 x Mahshuri. Australian Jurnal of Crop Science. 7(11): 1597-1605.

Rossman, A.Y., R.J. Howard, and B. Valent. 1990. Pyricularia grisea, the correct name for the rice blast disease fungus. Mycologia 82(4):509–512.

Sharma, T. R., A. K. Rai, S. K. Gupta, J. Vijayan, B. N. Devanna and S. Ray. 2012. Rice Blast Management Through Host-Plant Resistance: Retrospect and Prospect. Agric. Res. 1 (1): 37-52.

Sudir, Dini Yuliani, Anggiani Nasution, B. Nuryanto. 2013. Pemantauan penyakit utama padi sebagai dasar skrining ketahanan varietas dan rekomondasi pengendalian di beberapa daerah sentra produksi padi di Jawa. Laporan Hasil Penelitian Balai Besar Penelitian Tanaman Padi, Sukamandi. Th. 2013. 33p.

Sudir, A. Nasution, Santoso dan B. Nuryanto. 2014. Penyakit Blas Pyricularia grisea pada Tanaman Padi dan Strategi Pengendaliannya. Iptek Tanaman Pangan, 9 (2): 85-96.

Suganda, T., E. Yulia, F. Widiantini dan Hersanti. 2016. Intensitas penyakit blas (Pyricularia oryzae Cav.) pada varietas Ciherang di lokasi endemik dan pengaruhnya terhadap kehilangan hasil. Jurnal Agrikultura. 27(3): 154-159.

Tasliah, J. Prasetiyono, T. Suhartini dan I. H. Soemantri. 2015. Ketahanan galur-galur padi Pup1 terhadap penyakit blas. Jurnal Penelitian Pertanian Tanaman Pangan. 34(1): 29-36.

Wahab, M. I., Satoto, Rahmini, L. M. Zarwazi, Suprihatno, A. Guswara dan Suharna. 2018. Deskripsi Varietas Unggul Baru Padi. Badan Penelitian dan Pengembangan Pertanian, Kementerian Pertanian. Jakarta. 99p.

Wang, J. C., Y. Jia, J. W. Wen, W. P. Liu, X. M. Liu, L. Li, Z. Y. Jiang, J. H. Zhang, X. L. Guo and J. P. Ren. 2013. Identification of rice blast resistance gene using international monogenic differential. Crop Protection. 45: 109-116.

Yulianto. 2017. Pengendalian penyakit blas secara terpadu pada tanaman padi. Iptek Tanaman Pangan. 12(1):25-33.

Zulaika, B. P. Soekarno dan A. Nurmansyah. 2018. Pemodelan keparahan penyakit blas pada tanaman padi di Kabupaten Subang. Jurnal Fitopatologi Indonesia. 14(2): 47-53.




DOI: http://dx.doi.org/10.21082/jpptp.v3n1.2019.p1-8

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