Identifikasi dan Karakterisasi Manggis di Provinsi Bengkulu dan Bangka-Belitung
Abstract
ABSTRACT. Mansyah, E., M. Jawal A.S, I. Muas, Hendri, and F. Usman. 2007. Identification and Characterization of Mangosteen at Bengkulu and Bangka-Belitung Provinces. The research was conducted by exploration at mangosteen production center at Bengkulu and Bangka-Belitung Provinces from August 2003 to August 2004. The objective of this study was to find out the variability of mangosteen at Bengkulu and Bangka-Belitung Provinces. The parameters observed were morphology and genetic characters. Morphology characters observed were qualitative and quantitative while genetic observation was conducted by using RAPD technique. The results showed that mangosteen accessions at Bengkulu and Bangka-Belitung have morphology variations. The variations were on canopy and fruit shape. The canopy could be devided into 3 forms of pyramid, elliptical, and semicircular. Fruit shape described as round, flattened, ovoid, and irregular. RAPD analysis of 8 mangosteen accessions by using 2 primers OPH-13 (CACGCCACAC) and OPN-16 (AAGCGACCTG) showed that the accessions devided into 2 main groups with genetic similarity coefficient 0.78 to 1.0. First group consist of 8 genotypes which separated into 2 different genotypes with genetic similarity coefficient of 0.90. The second group consist of 2 genotypes with genetic similarity coefficient about 0.87. In general the 8 accessions could be separated into 4 different genotypes (1) BLT-03, BKL-01, and BKL-06, (2) BLT-08, BLT-12, and BKL-09, (3) BLT-10, and (4) BKL-07. Accessions grouping based on RAPD bands were not in accordance with morphology characters. Further evaluation by molecular marker was needed to clarify the genetic variability. This results strengthened the support about the presence of genetic variability on mangosteen.
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PDFDOI: http://dx.doi.org/10.21082/jhort.v17n2.2007.p%25p
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