Analisis Isozim dan Patogenisitas Isolat Cladosporium spp. Terhadap Karat Putih Pada Krisan (Isozyme Analysis and Pathogenicity of Cladosporium spp. Isolate Against White Rust on Chrysanthemum)

Evi Silvia Yusuf, I Djatnika

Abstract


Cladosporium spp. merupakan mikoparasit potensial untuk mengendalikan beberapa jenis cendawan karat. Penelitian bertujuan untuk mengetahui patogenisitas 10 isolat Cladosporium spp. yang ditemukan di daerah sentra krisan (Kabupaten Cianjur dan Bandung Barat) terhadap penyakit karat putih pada krisan dan hubungan kekerabatannya antara isolat Cladosporium spp. Percobaan dilakukan pada bulan April hingga Agustus 2014 di Laboratorium Mikologi Balai Penelitian Tanaman Hias (Balithi) dan Laboratorium Patologi dan Silvikultur, Fakultas Kehutanan, Institut Pertanian Bogor. Pada percobaan ini dilakukan uji patogenisitas 10 isolat Cladosporium spp. asal Kabupaten Cianjur dan Bandung Barat terhadap pustul karat pada daun krisan di laboratorium. Untuk menelusuri hubungan genetik antarisolat dilakukan analisis isozim secara elektroforesis dengan menggunakan enzim esterase (EST), acid phospatase (ACP), dan peroksidase (PER). Hasil percobaan menunjukkan sembilan isolat Cladosporoium spp. efektif memparasit pustul karat dengan efektivitas lebih dari 50%. Hasil analisis isozim menunjukkan terdapat dua kelompok Cladosporium spp. yang memiliki koefisien kemiripan 67%.

Keywords

Cladosporium spp.; Isozim; Mikoparasit; Patogenisitas

Abstract

Cladosporium spp. is a potential mycoparasite for controlling some rust fungi. The aim of the research was to obtain the effectiveness of 10 Cladosporium spp. isolates was found in chrysanthemum central area (Cianjur and West Bandung District) and genetic relationship among the isolats. The research carried on April to Agustus 2014  in Micology Laboratory Indonesian Ornamental Crop Institute and Pathology and Silviculture Laboratory, Faculty of Forestry, Bogor Agricultural University. Pathogenicity of the 10 fungus isolate was tested against rust pustules on chrysanthemum leaves and genetic relationship between isolates was analyzed by electrophoresis isozyme using EST, ACP, and PER enzyme. The results showed that nine of Cladosporoium spp. isolates were effective parasited rust pustule with more than 50% effectiveness. The cluster analysis based on isozyme analysis showed that Cladosporium spp. isolate have two  distinc groups with 67% similarity coefficient.

Keywords


Cladosporium spp.; Isozyme; Mycoparasite; Pathogenicity

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DOI: http://dx.doi.org/10.21082/jhort.v28n1.2018.p97-104

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