Perbandingan Teknik Inokulasi Puccinia horiana dan Seleksi Bakteri Antagonis untuk Mengendalikan Penyakit Karat Putih pada Krisan
Abstract
White rust disease caused by P. horiana is one of the serious problems on chrysanthemum cultivation. The pathogen causes yield losses up to 100%. The research was aimed (1) to determine the effective inoculation technique and (2) to select antagonistic bacteria for effectively controlling the pathogen. The research was carried out in the Laboratory and Glasshouse of Indonesian Ornamental Crops Research Institute (IOCRI), from June to December 2009. The research consisted of two experiments. Each experiments was arranged in a randomized completely block design with 11 treatments i.e. rust pustuls dipped in water, mature rust pustuls dipped in water, rust pustuls dipped in water and stored at 10oC during 12 hours, mature rust pustuls dipped in water and stored at 10oC during 12 hours, pustuls adhered on the leaf, mature pustuls adhered on the leaf, pustuls adhered beneath the leaf, mature rust pustuls adhered beneath the leaf, the plant + pustuls stored beside tested plants covered by transparent plastic, mature pustuls plants stored beside tested plants covered by transparent plastic, and control with three replications. The results indicated that the most effective inoculation technique for the pathogen was locating and infected plant with immature or mature pustuls surounding a healthy plant. The effective antagonistic bacteria against the pathogen was Corynebacterium-2. The effectiveness of the antagonistic bacteria in suppressing P. horiana was equivalent to synthetic fungicide azoksistrobin 0.1%. The Corynebacterium-2 isolate will be potentially used as an active ingredient of biopesticide for controlling white rust disease on chrysanthemum. The development of the biopesticide is expected to decrease to utilization of synthetic pesticides.
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PDFDOI: http://dx.doi.org/10.21082/jhort.v21n2.2011.p173-184
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