Kultur Apeks untuk Penyediaan Bibit Unggul Tebu Varietas PS864 dan PS881

Deden Sukmadjaja, Yati Supriyati, Saptowo J. Pardal


In vitro culture
techniques have become alternative to help overcome the
problems those are often encountered in the provision of
seeds through conventional means. Micropropagation
through apex culture in sugarcane has several advantages,
such as the produced plants have higher genetic stability,
high multiplication rate, and more healthy seeds (especially
virus-free)., The aims of the the research were to produce
seeds of two varieties of sugarcane, namely PS864 and
PS881, through apex culture. Laboratory-scale research was
conducted at the Indonesian Center for Agricultural
Biotechnology and Genetic Resources Research and
Development (ICABIOGRAD), Bogor, while sowing seeds
nursery was done in the Experimental Station of Gowa,
South Sulawesi Assessment Institute for Agricultural
Technology. The experiments consisted of initiation and
regeneration of apexes, shoots multiplication, rooting
induction, and acclimatization of plantlets. Research results
showed the initiation and regeneration of PS864 and PS881
through apex culture could be done on MS basic medium
containing 0.5 mg/l BAP. Shoot proliferation of both varieties
increased in the second subculture. Addition of 1 mg/l BAP
into medium in the second subculture resulted in higher
average number of shoots than that of 5 mg/l BAP, both for
PS864 and PS881. Addition of 1 mg/l and 5 mg/l kinetin
showed no significant differences for shoot numbers
compared to that of PS864 in medium containing 1 mg/l
BAP. The average number of PS881 shoots in multiplication
media containing 5 mg/l kinetin was higher than that of 1
mg/l kinetin. Increased concentrations of NAA and IBA from
0.1 mg/l to 0.5 mg/l in the MS medium were correlated to the
increased number of roots in PS864 shoots. Meanwhile, only
increased concentration of NAA that affected rooting percentage
of PS881. Acclimatization showed the percentage of
the plantlets grown in polybags was higher than that directly
grown in planting bed. The primary seeds (G0) produced in
these experiments were ready to be reproduced again to
obtain further stages.


Sugarcane (Saccharum spp.); apex culture; PS864; PS881.

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DOI: http://dx.doi.org/10.21082/jbio.v10n2.2014.p45-52


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