Identifikasi Perubahan Karakter Agronomis Padi Transgenik Penanda Aktivasi cv. Asemandi Generasi T1

Atmitri Sisharmini, Aniversari Apriana, Diah Nurmaliki, Tri Joko Santoso, Kurniawan R. Trijatmiko

Abstract


The activation-tagged populations of transgenic rice cv.
Asemandi have been developed by introducing construct of
activation taq Ac/Ds into rice genom of cv. Asemandi. The T1
transgenic rice populations cv. Asemandi containing
construct of activation taq have been obtained and needed
to be characterized. This study aimed to identify the Basta
herbicide resistant plants, transgenic plants containing hpt
and bar genes, and changes in agronomic traits. Basta
resistant plant was identified by treating leaf with basta
solution. Hpt and bar genes were detected by PCR using
specific primers. Phenotype characters were identified by
observing and measuring their agronomic parameters. The
study results showed that out of 315 rice transgenic cv.
Asemandi T1 treated with Basta solution, 176 (55.87%) plants
were indicated to be resistant to Basta. The results of PCR
analysis revealed that eight rice transgenic cv. Asemandi
tested contained both hpt and bar genes. In general,
compared to the nontransgenic plants, there were changes
in several agronomic parameters of T1 transgenic plants cv.
Asemandi, including plant height, days to flowering, days to
harvesting, periods of grain filling, and weight of 100 grains.
Correlation analysis showed that there was no correlation
between days of harvesting to weight of 100 grains in
transgenic rice, but there was correlation in nontransgenic
rice. Transgenic rice plants cv. Asemandi with changes in
the agronomic characters will be useful for further study,
such as to analyze the function of the genes.


Keywords


Rice; transgenic; activation tagging; gene identification.

Full Text:

PDF


DOI: http://dx.doi.org/10.21082/jbio.v9n3.2013.p107-116

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Jurnal AgroBiogen

 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


 

         

P-ISSN : 1907-1094
E-ISSN : 2549-1547


Jurnal AgroBiogen

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Jl. Tentara Pelajar 3A, Bogor 16111
Jawa Barat, Indonesia
Telp.: (0251) 8339793, 8337975
Call Center: 08211181677
Faks.: (0251) 8338820
E-mail: jurnal.agrobiogen@gmail.com
Situs: http://biogen.litbang.pertanian.go.id



View My Stats