Interaksi AtMEK1-EXGT pada Arabidopsis thaliana pada Saat Terjadi Pelukaan

Toto Hadiarto, Fumio Nanba

Abstract


Protein
interactions occur within cellular level of stimulated plant
cells to relay signals from receptors to production of response.
AtMEK1-EXGT interaction had been detected in nontreated
Arabidopsis. In this research, interaction between
AtMEK1, a mitogen-activated protein kinase kinase of
Arabidopsis thaliana, and EXGT, endoxyloglucan transferase,
after the plant was wounded was examined using
co-immunoprecipitation and in vitro phosphorylation assay.
The results demonstrated that EXGT interact with AtMEK1
soon after and 10 minutes after wounding. In addition,
AtMEK1 phosphorylation activity increased when increased
level of EXGT was incorporated into the reaction mixture.
These indicate that EXGT amplifies wound-caused phosphorylation
activity of AtMEK1. The results elucidate part of
the AtMEKK1-AtMEK1-AtMPK4 cascade which is stimulated
by wounding. How the complex interaction between EXGT,
AtMEK1 and AtMPK4 fits within the cascade is remained to
be uncovered.


Keywords


Endoxyloglucan transferase; AtMEK1; MAPK; wounding.

Full Text:

PDF


DOI: http://dx.doi.org/10.21082/jbio.v4n2.2008.p59-64

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Jurnal AgroBiogen

 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


 

         

P-ISSN : 1907-1094
E-ISSN : 2549-1547


Jurnal AgroBiogen

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Jl. Tentara Pelajar 3A, Bogor 16111
Jawa Barat, Indonesia
Telp.: (0251) 8339793, 8337975
Call Center: 08211181677
Faks.: (0251) 8338820
E-mail: jurnal.agrobiogen@gmail.com
Situs: http://biogen.litbang.pertanian.go.id



View My Stats