Identifikasi Marka Polimorfik untuk Pemuliaan Padi Toleran Defisiensi Fosfor

Joko Prasetiyono, Hajrial Aswidinoor, Sugiono Moeljopawiro, Didy Sopandie, Masdiar Bustamam

Abstract


Information on polymorphisms
among rice parents are very important in rice
breeding for tolerance to phosphorus defficiency. A study
was conducted at the Molecular Biology Laboratory,
Indonesian Center Agricultural Biotechnology and Genetic
Resources (ICABIOGRAD) from October 2006 to July 2007 to
identify polymorphism markers from 6 rice genotypes. The
rice genotypes, i.e., Dodokan, Situ Bagendit, Batur, Kasalath,
NIL-C443, dan K36-5-1-1 were analyzed for polymorphisms
using 496 SSR markers, which cover the rice genomes.
Seven of the 496 markers were used as foreground and
recombinant selection markers, and the rests (489 markers)
were used as background selection markers. PCR amplifications
were separated on a 5% polyacrylamide gel and
colored by the silver staining method. Three different markers
among the seven foreground and recombinant selection
markers were selected from each crossing, which are
tightly linked with Pup1 gene and have a distance less than 5
cM. These markers are Dodokan vs Kasalath (RM277, SSR3,
RM519), Dodokan vs NIL-C443 (RM277, SSR3, RM519),
Dodokan vs K36-5-1-1 (RM277, SSR3, RM519), Situ Bagendit
vs Kasalath (RM28102, SSR3, RM519), Situ Bagendit vs NILC443
(RM28102, SSR3, RM519), Situ Bagendit vs K36-5-1-1
(RM511, SSR3, RM519), Batur vs Kasalath (RM277, RM1261,
RM519), Batur vs NIL-C443 (RM277, RM1261, RM519), and
Batur vs K36-5-1-1 (RM28102, SSR3). Variations in background
selection primers were found in each chromosome
and in each parent combinations. Primers on chromosome
4, 5, and 12 showed the lowest polymorphisms; more
primers are needed for these chromosomes.


Keywords


Microsatellite markers; rice breeding; phosphorus defficiency.

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DOI: http://dx.doi.org/10.21082/jbio.v4n2.2008.p51-58

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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