Regenerasi Jeruk Siam melalui Embriogenesis Somatik

Ali Husni, Agus Purwito, Ika Mariska, Sudarsono Sudarsono


The Regenerated of Siam Tangerin through Somatic
Embryogenesis. Ali Husni, Agus Purwito, Ika Mariska,
and Sudarsono. Somatic embryogenesis occurs in most
plants that are cultured on a suitable medium in vitro.
Somatic embryo may arise from single cells and the embryogenic
cells are widely applicable in plant propagation,
genetic manipulation and transgenic technologies. The
present study was carried out to develop an effective
somatic embryogenesis technique to regenerate Siam
tangerine plants. Materials used in this study were nucellar
tissues of young fruits (30-90 days post anthesis). Induction
of embryogenic calli was done by culturing the tissues on
three different basal media (MS, MW and MT). Embryo
maturation was done on the MW medium + ABA (0; 0.1; 0.3;
dan 0.5 mg/l), while germination to plantlet development
was done on WS medium + GA3 (0, 0.1, 0.3, and 0.5 mg/l).
The results showed that among the three media, MW was
the best medium for callus induction from nucellar tissues
with a success rates 98% for Simadu and 100% for
Pontianak. The best maturation of embryo somatic was
done on MW medium + ABA 0.5 mg/l with success rates
99%, while the best medium for germination and development
into planlets was MW medium + 0.5 mg/l GA3 with a
success rate 58%.


In vitro regeneration; Siam tangerin; somatic embryogenesis



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P-ISSN : 1907-1094
E-ISSN : 2549-1547

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