Introduksi Gen DefH9-iaaM dan DefH9-RI-iaaM ke dalam Genom Tanaman Tomat Menggunakan Vektor Agrobacterium tumefaciens

Ragapadmi S Purnamaningsih

Abstract


Introduction of DefH9-iaaM and DefH9-RI-iaaM Gene
Into Tomato Genome Using Agrobacterium tumefaciens.
Ragapadmi Purnamaningsih. Plant genetic improvement
can be conducted through genetic engineering.
Parthenocarpic fruit production could increase fruit
production and its qulities. IAA genes were introduced into
three tomato cultivars Ratna, Opal and LV 6117 using two
constract genes DefH9-iaaM and DefH9-RI-iaaM. The iaaM
gene is able to increase auxin biosynthesis in transgenic
plant cells and organs because indol-eacetamide,
synthesized by the product of the iaaM gene, is converted
either chemically or enzimatically to indole-3-acetic acid
(IAA), while the promotor DefH9 enable IAA gene expressed
specifically in the ovules. The objectives of this experiment
was to identify gene introduction into plant genom of three
tomato cultivars. The factors tested were two constract of
IAA genes (DefH9-iaaM or DefH9-RI-iaaM), tomato cultivars
(Ratna, Opal, and LV 6117) and time of explant inoculation
(5, 15, 30 minute). The result showed that the best time
inoculation was 5 minute. Otherwise three tomato cultivars
response better to DefH9-RI-iaaM than DefH9-iaaM. The total
efficiency of regeneration and total efficiency of
transformation of both genes were 25.38% and 20.32%. PCR
analysis showed that 10 plant have positive PCR, were 1
plant carried (Opal) DefH9-iaaM gene and 9 plant (Ratna,
Opal, LV 6117) carried DefH9-RI-iaaM gene.


Keywords


Transformation; tomato; genetic engineering

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DOI: http://dx.doi.org/10.21082/jbio.v6n1.2010.p18-25

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