Confirmation of Alleles Inheritance in F1 Progenies Derived from a Cross of Calcutta-4 (Musa acuminata ssp. burmannicoides) and Musa acuminata ssp. microcarpa Based on SSR Markers

Dea Rosalia, Puji Lestari, Andy Soegianto, Darmawan Saptadi, Agus Sutanto, Kristianto Nugroho, Rerenstradika T. Terryana, I Made Tasma, Ika Roostika

Abstract


Banana breeding to produce improved varieties with disease resistance haracters and other desired traits could sustain its yield. Alleles harbored by parents could be passed on to the offsprings through hybridization, but need to be confirmed using molecular markers. This study aimed to confirm allele inheritance in F1 progenies derived from a cross of Calcutta-4 (Musa acuminata ssp.  burmannicoides) and M. acuminata ssp. microcarpa based on SSR markers. Eleven pairs of SSR primers were used to amplify DNA of 44 progenies using the PCR technique. The results showed that six SSR markers (MaSSR 1.1, MaSSR
5.1, MaSSR 6.1, MaSSR 7.1, MaSSR 8.1, and MaSSR 11.1) were polymorphic in both parents. Four markers (MaSSR 1.1, MaSSR 5.1, MaSSR 6.1, and MaSSR 8.1) had PIC >0.7, indicating their informativeness to distinguish these progenies and other genetic studies of banana germplasms. A total of 44 F1 individuals were confirmed to harbor alleles inherited from their parents,
suggesting as true progenies from the cross of Calcutta-4 and M. acuminata ssp. microcarpa. This population demonstrated 100% success of hybridization performed. Chi-Square analysis revealed that segregation of all markers did not match to Mendelian ratio 1:2:1, except for MaSSR 1.1 (x2 = 5,62) and MaSSR 6.1 (x2 = 3,77) markers. The genetic traceability of banana F1 progenies demonstrating the usefulness and feasibility of SSR markers in this study provided information on selection of true progenies which may be valuable for breeders to assist selection process in future banana breeding program in Indonesia.


Keywords


Banana; cross; allele,;F1 population

Full Text:

PDF

References


Balai Penelitian Tanaman Buah Tropika (2009) Teknologi pengendalian penyakit layu pisang dan penerapannya di lapangan. Pertemuan Rehabilitasi Kebun Pisang dan POKJA Penanggulangan Penyakit Layu Pisang. Tanjungkarang, 28 Juli.

Changadeya, W., Kaunda, E. & Ambali, A.J.D. (2012) Molecular characterisation of Musa L. cultivars cultivated in Malawi using microsatellite markers.

African Journal of Biotechnology, 11 (18), 4140–4157.

Christelová, P., Valárik, M., Hřibová, E., Van den Houwe, I., Channelière, S., Roux, N. & Dolezel, J. (2011) A platform for efficient genotyping in Musa using microsatellite markers. AoB Plants, 11 (1), 1–14.

De Langhe, E., Pillay, M., Tenkouano, A. & Swennen, R. (2005) Integrating morphological and molecular taxonomy in Musa: The African plantains (Musa spp. AAB group). Plant Systematics and Evolution, 255, 225–236.

Demore, P.D.S., Uneda-Trevisoli, S.H., Di Mauro, A.O., Morceli, T.G.S., Muniz, F.R.S., Costa, M.M., Sarti, D.G.P. & Mancini, M.C. (2009) Validation of microsatellite markers for assisted selection of soybean

genotypes resistant to powdery mildew. Crop Breeding and Applied Biotechnology, 9, 45–51.

Doloiras-Larano, A., Garcia, R.N., Sandoval, C.M.C., Lalusin, A.G., Gueco, L.S., Huelgas, V.C. & Tecson- Mendoza, E.M. (2018) DNA fingerprinting and genetic diversity analysis using of Philippine saba and other

cultivars of Musa balbisiana Colla using simple sequence repeat markers. Philippine Journal of Crop Science, 43 (2), 1–11.

Dongre, A.B., Raut, M.P., Bhandarkar, M.R. &. Meshram, K.J. (2011) Identification and genetic purity testing of cotton F1 hybrid using molecular markers. Indian Journal of Biotechnology, 10, 301–306.

Doyle, J.J. & Doyle, J.L. (1990) Isolation of plant DNA from fresh tissue. Coccus, 12, 13–15.

Elci, E. & Hancer, T. (2015) Genetic analysis of maize (Zea mays L.) hybrids using microsatellite markers. Tarim Bilimeri Dergisi-Journal of Agricultural Sciences, 21, 192–198.

Handayani, T., Martanti, D., Poerba, Y.S. & Witjaksono (2017) Deteksi awal ketahanan beberapa aksesi pisang lokal dan hasil persilangan terhadap penyakit layu Fusarium (Foc VCG 01213/16 TR4). Jurnal Hortikultura Indonesia, 8 (2), 88–96.

Hermanto, C., Sutanto, A., Jumjunidang, Edison, H.S., Daniells, J.W., O'Neil, W., Sinohin, V.G., Molina, A.B. & Taylor, P. (2011) Incidence and distribution of Fusarium wilt disease of banana in Indonesia. Acta Horticulturae, 897, 313–322.

Hildebrand, C.E., Torney, D. & Wagner, R.P. (1992) Informativeness of polymorphic DNA markers. Los Alamos Science, 17 (3), 233–238.

Lestari, P., Sustiprijatno & Asadi (2017) Konfirmasi penurunan

alel tetua persilangan kedelai pada generasi F1 berdasarkan marka SSR. Prosiding Seminar Nasional III Tahun 2017. Biologi, Pembelajaran, dan Lingkungan Hidup Perspektif Interdisipliner. 29 April 2017: Prodi Pendidikan Biologi-FKIP bekerja sama dengan Pusat Studi Lingkungan dan Kependudukan (PSLK), Malang.

Li, L.F., Häkkinen, M., Yuan, Y.M., Hao, G. & Ge, X.J. (2010) Molecular phylogeny and systematics of the banana family (Musaceae) inferred from multiple nuclear and chloroplast DNA fragments, with a special

reference to the genus Musa. Molecular Phylogenetics and Evolution, 57 (1), 1–10.

Liu, K. & Muse, S.V. (2005) PowerMarker: An integrated analysis environment for genetic marker analysis. Bioinformatics, 21 (9), 2128–2129.

Molina, A.B., Williams, R.C., Hermanto, C., Suwanda, Komolong, B. & Kokoa, P. (2010) Mitigating the threat of banana Fusarium wilt: Understanding of agroecological distribution of pathogenic forms and

developing disease management strategies. ACIAR Publication.

Nyine, M., Uwimana, B., Swennen, R., Batte, M., Brown, A., Christelova, P., Hribova, E., Lorenzen, J. & Dolozel, J. (2017) Trait variation and genetic diversity in a banana genomic selection training population. PLoS ONE, 12 (6), 1–23.

Oriero, C. E., Odunola, O.A., Lokko, Y. & Ingelbrecht I. (2006) Analysis of B-genome derived simple sequence repeat (SSR) markers in Musa spp. Journal of Biotechnology, 5 (2), 126–128.

Pancoro, A., Septiyani, T.A., Indriyani, N.L.P. & Santoso, P.J. (2016) Analisis progeni F1 hasil persilangan intra dan interspesies durian (Durio sp.) menggunakan marka mikrosatelit. Jurnal Hortikultura, 26 (2), 171–180.

Pusat Genom Pertanian Indonesia (2015) Pusat Genom Pertanian Indonesia (PGPI). [Online] Tersedia pada: http://genom.litbang.pertanian.go.id/hortikultura/index/ php/primer/index/4/ssr) [Diakses 27 November 2018].

Ravishankar, K.V., Raghavendra, K.P., Athani, V., Rekha, A., Sudeepa, K., Bhavya, D., Srinivas, V. & Ananad, L. (2013) Development and characterisation of microsatellite markers for wild banana (Musa

balbisiana). Journal of Horticultural Science and Biotechnology, 88 (5), 605–609.

Reflinur, Kim, B., Jang, S.M., Chu, S.H., Bordiya, Y., Akter, Md.B., Lee, J., Chin, J.H. & Koh, H.J. (2014) Analysis of segregation distortion and its relationship to hybrid barriers in rice. Rice, 7 (3), 1–12.

Rotchanapreeda, T., Wongniam, S., Swangpol, S.C., Chareonsap, P.P., Sukkaewmanee, N. & Somana, J. (2016) Development of SSR markers from Musa balbisiana for genetic diversity analysis among Thai

bananas. Plant Systematics and Evolution, 302 (7), 739–761.

Santana, F.A., da Silva, M.F., Guimaraes, J.K.F., Ferreira, M.F.S., Pereira, W.D., Piovesan, N.D. & de Barros, E.G. (2014) Marker-assisted selection strategies for developing resistant soybean plants to cyst nematode. Crop Breeding and Applied Biotechnolog, 14 (3), 180–

Santoso, P.J., Djamas, N., Rebin & Pancoro, A. (2014) Analisis diversitas dan paternitas progeni F1 hasil persilangan Arumanis 143 x Mangga Merah menggunakan marka mikrosatelit. Jurnal Hortikultura, 24 (3), 210–219.

Subashini, V., Shanmugapriya, A. & Yasodha, R. (2013) Hybrid purity assessment in Eucalyptus F1 hybrids using microsatellite markers. Biotechnology, 4 (4), 367–373.

Vincent, K., Robert, K., Morag, F., Tadeo, K., Yona, B. & Peter, V. (2014) Identification of F1 cassava (Manihot esculenta) progeny using microsatellite markers and capillary electrophoresis. American Journal of Plant Science, 5 (1), 119–125.

Wahyuningtyas, W., Retnoningsih, A. & Rahayu, E.S. (2009) Keanekaragaman genetika pisang bergenom B berdasarkan penanda mikrosatelit. Biosaintifika: Journal of Biology & Biology Education, 1 (1), 1–10.

Zuo, C., Deng, G., Li, B., Huo, H., Li, C., Hu, C., Kuang, R., Yang, Q., Dong, T., Sheng, O. & Yi, G. (2018) Germplasm screening of Musa spp. for resistance to Fusarium oxysporum f. sp. cubense tropical race 4 (Foc

TR4). European Journal of Plant Pathology, 151, 723–734.




DOI: http://dx.doi.org/10.21082/jbio.v16n1.2020.p17-24

Refbacks

  • There are currently no refbacks.




Copyright (c) 2020 Jurnal AgroBiogen

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


 

         

P-ISSN : 1907-1094
E-ISSN : 2549-1547


Jurnal AgroBiogen

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Jl. Tentara Pelajar 3A, Bogor 16111
Jawa Barat, Indonesia
Telp.: (0251) 8339793, 8337975
Call Center: 08211181677
Faks.: (0251) 8338820
E-mail: jurnal.agrobiogen@gmail.com
Situs: http://biogen.litbang.pertanian.go.id



View My Stats