The Potency of Endophytic Bacteria Isolated From Red Ginger to Enhance Black Pepper Seedlings Growth

Andriana Kartikawati, NFN Gusmaini

Abstract


Endophytic bacteria have many benefits such as phosphate solubilization, nitrogen  fixation, increasing mineral absorption as well as enhancing plant resistance against diseases. The research aimed to obtain endophytic bacteria potential to stimulate the growth of black pepper seedling. The first step was isolating endophytic bacteria from roots, rhizomes, and leaves of healthy red ginger. Characterization and selection were conducted by evaluating the capability on phosphate solubilization, nitrogen fixation, and indole acetic acid (IAA) hormone production of the isolated endophytic bacteria. There were 36 isolates consisted of 14 isolates from leaves, 9 isolates from roots and 13 isolates from rhizomes. Seventeen isolates were capable to dissolve phosphate, fix nitrogen and produce IAA or possessing those three characters, but only nine isolates were tested on pepper seedlings. The nine isolates were Ajc 1, Ajc 3, Ajc 7, Ajc 9, Rjc 1, Rjc 6, Djc 3, Djc 6, and Djc 11. Further evaluation of the nine isolates which were inoculated to pepper seedlings, indicated that Rjc1 isolate enhanced pepper seedlings height better than other isolates.

Keywords


Piper nigrum; Zingiber officinale var rubrum; endophytic bacteria

Full Text:

PDF

References


Anggara, B.S. & Lisdiana, L. (2014) Isolasi dan Karakterisasi Bakteri Endofit Penghasil Hor-mon Indole Acetic Acid dari Akar Tanaman Ubi Jalar. LenteraBio. 3 (3), 160-167.

Aravind, R., Dinu, A., Eapen, S.J., Kumar, A. & Ramana, K. V (2009a) Isolation and Evaluation of Endophytic Bacteria Against Plant Parasitic Nematodes Infesting Black Pepper (Piper nigrum L.). Indian Journal of Nematology. 39 (2), Nematological Society of India, 211-217.

Aravind, R., Kumar, A., Eapen, S.J. & Ramana, K. V. (2009b) Endophytic Bacterial Flora in Root and stem Tissues of Black Pepper (Piper nigrum L.) Genotype: Isolation, Identification and Evaluation Against Phytophthora capsici. Letters in Applied Microbiology. 48 (1), 58-64. doi:10.1111/j.1472-765X.2008.02486.x.

Arshad, M. & Frankenberger Jr, W.T. (1991) Microbial Production of Plant Hormones. In: The rhizosphere and plant growth. Springer, pp. 327-334.

Benizri, E., Courtade, A., Picard, C. & Guckert, A. (1998) Role of Maize Root Exudates in the Production of Auxins by Pseudomonas fluorescens M. 3.1. Soil Biology and Biochemistry. 30 (10), Pergamon, 1481-1484.

Edward, E.J., King, W.S., Teck, S.L.C., Jiwan, M., Aziz, Z.F.A., Kundat, F.R., Ahmed, O.H. & Majid, N.M.A. (2013) Antagonistic Activities of Endophytic Bacteria Against Fusarium Wilt of Black Pepper (Piper nigrum). International Journal of Agriculture & Biology. 15 (2), 291-296.

Gusmaini, Aziz, S.A., Munif, A., Sopandie, D. & Bermawie, N. (2013) Isolation and Selection of Endophytic Bacteria Consortia from Medicinal Plant (Andrographis paniculata) as Plant Growth Promoting Agents. Journal of Agronomy. 12 (3), 113-121.

Gusmaini & Kartikawati, A. (2015) Isolasi dan Karakterisasi Bakteri Endofit Berasal dari Tanaman Lada Bangka. Prosiding Seminar Teknologi Cengkeh, Lada dan Pala. (3), 159-168.

Hallmann, J., Quadt-Hallmann, A., Mahaffee, W.F. & Kloepper, J.W. (1997) Bacterial Endophytes in Agricultural Crops. Canadian Journal of Microbiology. 43 (10), 895-914. doi:10.1139/m97-131.

Hidayati, U., Chaniago, I.A., Munif, A. & Santosa, D.A. (2014) Potency of Plant Growth Promoting Endophytic Bacteria from Rubber Plants (Hevea brasiliensis Mull. Arg). Journal of Agronomy 13(3):147-152.

Holt, J.G., Krieg, N.R., Sneath, P.H.A. al, Staley, J.T. & Williams, S.T. (1994) Bergey’s Manual of Determinate Bacteriology. Baltimore, MD (USA) Williams & Wilkins. p 1-918.

Hussain, A. & Shakoor, A. (1995) Effect of Soil Applied L-Tryptophan on Growth and Chemical Composition of Cotton. Journal of Plant Nutrition. 18 (2), 317-329. doi:10.1080/01904169509364904.

Jha, P.N., Gupta, G., Jha, P. & Mehrotra, R. (2013) Association of Rhizospheric/Endophytic Bacteria with Plants: A Potential Gateway to Sustainable Agriculture. Greener Journal of Agricultural Sciences. 3 (2), 73-84.

Kaga, H., Mano, H., Tanaka, F., Watanabe, A., Kaneko, S. & Morisaki, H. (2009) Rice Seeds as Sources of Endophytic Bacteria. Microbes and Environments. 24 (2), 154-162. doi:10.1264/jsme2.ME09113.

Krishnamoorthy, N. (1981) Plant Growth Substances. New Delhi, Tata Mc Grawhill Publishing Company Limited. pp. 1-187.

Long, H.H., Schmidt, D.D. & Baldwin, I.T. (2008) Native bacterial endophytes promote host growth in a species-specific manner; phytohormone manipulations do not result in common growth responses. PLoS One. 3 (7), Public Library of Science, e2702.

Mattos, K.A., Pádua, V.L.M., Romeiro, A., Hallack, L.F., Neves, B.C., Ulisses, T.M.U., Barros, C.F., Todeschini, A.R., Previato, J.O. & Mendonça-Previato, L. (2008) Endophytic Colonization of Rice (Oryza sativa L.) by the Diazotrophic Bacterium Burkholderia kururiensis and Its Ability to Enhance Plant Growth. Anais da Academia Brasileira de Ciencias. 80 (3), 477-493. doi:10.1590/S0001-37652008000300009.

Munif, Abdul, Suryo Wiyono, and Suwarno. 2012. “Isolasi Bakteri Endofit Asal Padi Gogo dan Potensinya Sebagai Agens Biokontrol dan Pemacu Pertumbuhan.” Fitopatologi Indonesia 8 (3), 57-64.

Nassar, A.H., El-Tarabily, K.A. & Sivasithamparam, K. (2005) Promotion of Plant Growth by an Auxin-Producing Isolate of the Yeast Williopsis saturnus Endophytic in Maize (Zea mays L.) Roots. Biology and Fertility of soils. 42 (2), Springer, 97-108.

Patten, C.L. & Glick, B.R. (2002) Role of Pseudomonas putida Indoleacetic Acid in Development of the Host Plant Root System. Applied and environmental microbiology. 68 (8), 3795-801. doi:10.1128/AEM.68.8.3795.

Petrini, O. (1991) Fungal Endophytes of Tree Leaves. In: Andrews J.H., Hirano S.S. (eds) Microbial Ecology of Leaves. Brock/Springer Series in Contemporary Bioscience. New York, NY, Springer. pp. 179-197.

Ponmurugan, P. & Gopi, C. (2006) In Vitro Production of Growth Regulators and Phosphatase Activity by Phosphate Solubilizing Bacteria. African Journal of Biotechnology. 5 (4), Academic Journals (Kenya), 348-350.

Prakamhang, J. (2007) Microbial Communities and Their nifh Gene Expression in Rice Endophytic Diazotroph Bacteria. Suranaree University of Technology. Thailand. pp. 1-97.

Premono, M.E., Moawad, A.M. & Vlek, P.L.G. (1996) Effect of Phosphate-Solubilizing Pseudomonas putida on the Growth of Maize and Its Survival in the Rhizosphere. Indonesian J. Crop Sci. 11, 11-23.

Rosenblueth, M. & Martínez-Romero, E. (2006) Bacterial Endophytes and Their Interactions with Hosts. Molecular Plant-Microbe Interactions. 19 (8), 827-837. doi:10.1094/MPMI-19-0827.

Rostiana, O., Abdullah, A. & Taryono, H.E.A. (1991) Jenis-jenis Tanaman Jahe. Edisi Khusus Littro. 7, 7-10.

Spaepen, S., Vanderleyden, J. & Remans, R. (2007) Indole-3-Acetic Acid in Microbial and Microorganism-Plant Signaling. FEMS Microbiology Reviews. 31 (4), 425-448. doi:10.1111/j.1574-6976.2007.00072.x.

Sumiasri, N. & Indarto, N.S. (2001) Tanggap Setek Cabang Bambu Betung (Dendrocalamus asper) pada Penggunaan Berbagai Dosis Hormon IAA dan IBA, J. Nature Indonesia. 3 (2), 121-128.

Susilowati, D., Saraswati, R., Hastuti, R & Yuniarti, E. (2007) Peningkatan Serapan N pada Kedelai yang Diinokulasi Bakteri Diazotrof Endofit di Medium Vermiculit. Jurnal tanah dan Iklim. 26, 41-46.

Taechowisan, T., Lu, C., Shen, Y. & Lumyong, S. (2005) Secondary Metabolites from Endophytic Streptomyces aureofaciens CMUAc130 and Their Antifungal Activity. Microbiology. 151 (5), 1691-1695. doi:10.1099/mic.0.27758-0.

Thakuria, D., Talukdar, N.C., Goswami, C., Hazarika, S., Boro, R.C. & Khan, M.R. (2004) Characterization and Screening of Bacteria from Rhizosphere of Rice Grown in Acidic Soils of Assam. Current Science. JSTOR, 978-985.

Wahyuno, D., Florina, D. & Manohara, D. (2017) Fungal Endophytes of Pepper For Improving Growth and Suppressing. Bulittro. 28 (1), 57-64.

War Nongkhla, F.M. & Joshi, S.R. (2014) Epiphytic and Endophytic Bacteria that Promote Growth of Ethnomedicinal Plants in the Subtropical Forests of Meghalaya, India. Revista de Biología Tropical. 62 (4), 1295. doi:10.15517/rbt.v62i4.12138.




DOI: http://dx.doi.org/10.21082/bullittro.v29n1.2018.37-46

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2018 Buletin Penelitian Tanaman Rempah dan Obat

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


View My Stats

Creative Commons License
Buletin Penelitian Tanaman Rempah dan Obat is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Based on a work at http://ejurnal.litbang.pertanian.go.id/index.php/bultro

Buletin Penelitian Tanaman Rempah dan Obat (BUL LITTRO) Editorial Office :

Indonesian Spices and Medicinal Crops Research Institute (ISMCRI)
Jl. Tentara Pelajar No. 3 Bogor, Indonesia 16111
Telp. (0251) 8321879, Fax. (0251) 8327010
E mail : buletintro@gmail.com
Website : http://balittro.litbang.pertanian.go.id