The Effect of Essential Oil Formulas on Mortality and Oviposition Deterrent of Helopeltis antonii

Tri Lestari Mardiningsih, NFN Ma'mun


Citronella, clove and lemongrass oils are known to be toxic and repel certain pests.  Mixing different essential oils (EOs) in the form of formula is expected to increase the insecticidal properties. The experiments aimed to examine the effect of EOs mixture formulas on mortality and oviposition deterrent of Helopeltis antonii. The study was conducted at the green house of Indonesian Spices and Medicinal Crops Research Institute. A mixture of two EOs citronella + clove (1:1), lemongrass + clove (1:1), individual EO formulas, and their inert solution (mixture of tween 80, turpentine, and teepol) were tested on H. antonii at 5 and 10 ml.l-1 concentrations.  Parameters observed were the number of eggs laid, mortality of adults and nymphs of H. antonii. The citronella + clove and lemongrass + clove formula at 10 ml.l-1 deterred the oviposition of  H. antonii by 60.18 % and 46.56 % respectively. These formulas also caused adults mortality at 79.17 % and 62.50 %, as well as the nymphs mortality at 87.50 % and 82.50 % respectively. The citronella + clove and lemongrass + clove formulas tested had the same effectiveness compared to the commercial citronella oil formula. Therefore, these two formulas were potential to be tested in field to control H. antonii.


Helopeltis antonii; essential oils; mortality; oviposition deterrent

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Akhtar, Y., Yeoung, Y. & Isman, M. (2008) Comparative Bioactivity of Selected Extracts from Meliaceae and Some Commercial Botanical Insecticides against Two Noctuid Caterpillars, Trichoplusia ni and Pseudaletia unipuncta. Phytochem. Rev. 7, 77-88.

Balfas, R. & Mardiningsih, T. (2016) Pengaruh Minyak Atsiri terhadap Mortalitas dan Penghambatan Peletakan) Crocidolomia pavonana F. Bul. Littro. 27 (1), 85-92.

Bhuiyan, M., Begum, J., NC Nandi, N. & Akter, F. (2010) Constituents of the Essential Oil from Leaves and Buds of Clove (Syzigium caryophylatum) (L.) Alston). African Journal of Plant Science. 4 (11), 451-454.

BSN (2006) SNI 06-2387-2006. Minyak Daun Cengkih. Jakarta, Badan Standardisasi Nasional. 8 hlm.

BSN (1995) SNI 06-3953-1995. Minyak Sereh. Jakarta, Badan Standardisasi Nasional. 14 hlm.

Isman, M., Miresmailli, S. & Machial, C. (2011) Commercial Opportunities for Pesticides Based on Plant Essential Oils in Agriculture, Industry, and Consumer Products. Phytochem. Rev. 10, 197-204.

Karmawati, E. (2010) Pengendalian Hama Helopeltis spp. pada Tanaman Jambu Mete Berdasarkan Ekologi; Strategi dan Implementasinya. Pengembangan Inovasi Pertanian. 3 (2), 102-119.

Karmawati, E. & Mardiningsih, T. (2005) Hama Helopeltis spp. pada Jambu Mete dan Pengendaliannya. Perkembangan Teknologi Tanaman Rempah dan Obat XVII (1):1-6. XVII (1), 1-6.

Khater, H. (2012) Prospects of Botanical Biopesticides in Insect Pest Management. Pharmacologia. 3 (12), 641-656.

Koul, O., Walia, S. & Dhaliwal, G. (2008) Essential Oils as Green Pesticides: Potential and Constraints. Biopestic. Int. 4 (1), 63-84.

Kumar, P., Misra, S., Malik, A. & Satya, S. (2011) Repellent, Larvicidal, and Pupicidal Properties of Essential Oils and Their Formulations against the Housefly, Musca domestica. Medical and Veterinary Entomology. 25 (3), 302-310.

Mardiningsih, T. & Balfas, R. (2017) Effect of Essential Oil Combination on Mortalities and Oviposition Deterrents of Crocidolomia pavonana and Helopeltis antonii. Bul. Littro. 28 (1), 75-88.

Olivero-Verbel, J., Nerio, L. & Stashenko, E. (2010) Bioactivity against Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) of Cymbopogon citratus and Eucalyptus citriodora Essential Oils Grown in Colombia. Pest Manag. Sci. 66 (6), 664-668.

Rohimatun & Laba, I. (2013) Efektivitas Insektisida Minyak Serai Wangi dan Cengkeh terhadap Hama Pengisap Buah Lada (Dasynus piperis China). Bul. Littro. 24 (1), 26-34.

Rusli, S. (2002) Dasar-dasar Penyulingan Minyak Atsiri. Balai Penelitian Tanaman Rempah dan Obat. 20 hlm.

Setiawati, W., Murtiningsih, R. & Hasyim, A. (2011) Laboratory and Field Evaluation of Essential Oils from Cymbopogon nardus as Oviposition Deterrent and Ovicidal Activities against Helicoverpa armigera Hubner on Chili Pepper. Indonesia Journal of Agricultural Science. 12 (1), 9-16.

Siswanto, Muhamad, R., Omar, D. & Karmawati, E. (2008) Life Tables and Population Parameters of Helopeltis antonii (Hemiptera: Miridae) Reared on Cashew (Anacardium occidentale L.). Journal of Bioscience. 19 (1), 91-101.

Sulistyowati, E., Ghorir, M., Wardani, S. & Purwoko, S. (2014) Keefektifan Serai, Bawang Putih, dan Bunga Paitan sebagai Insektisida Nabati terhadap Pengisap Buah Kakao, Helopeltis antonii. Pelita Perkebunan. 30 (1), 35-46.

Tajidin, N.E., Ahmad, S.H., Rosenani, A.B., Azimah, H. & Munirah, M. (2012) Chemical Composition and Citral Content in Lemongrass (Cymbopogon citratus) Essential Oil at the Three Maturity Stages. African Journal of Biotechnology. 11 (11), 2685-2693. doi:10.5897/AJB11.2939.

Tripathi, A., Upadhyay, S., Bhuiyan, M. & Bhattacharya, P. (2009) A Review on Prospects of Essential Oils as Biopesticides in Insect-Pest Management. Journal of Pharmacognosy and Phytotherapy. 1 (5), 52-63.

USA EO Association (1970) EOA Specification and Standards Oil of Lemongrass No 7. New York. 67 p.

Willis, M., Rohimatun, Laba, I. & Nurjanani (2013) Control of Cocoa Pod Borer (Conopomorpha cramerella) and Cocoa Pod Sucker (Helopeltis sp.) Using Essential Oil-base Insecticides. In: Rostiana,O. et al. (eds.) Proceedings of the International Seminar on Spices Medicinal and Aromatic Plants. Jakarta, Indonesia Agency for Agricultural Research and Development (IAARD), pp. 115-120.



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