Aklimatisasi Bibit Kapolaga Asal Kultur Jaringan

Ali Husni, NFN Hobir, D. Sukmadjaja

Abstract


Aclimatitation of cardamom seeding from tissue culture

The success of seedling production by tissue culture in influenced by several factors, one of which is acclimatization process. In this study, effect of growing medium and acclimatization period on the growth of cardamom were evaluated. The study was conducted in the laboratory and glass house of the research Institute and Development Centre for Industrial Crops from October 1993 to June 1994. The experiment was carried out in two steps. The first step was to evaluated the effect of sterilization of media on the growth of plantlet. While the second one was to evaluated the effect of acclimatization period on the growth of plants in polybag. The experiments was carried out with a completely randomized design with three replicates. Results showed that sand medium either sterilized or not produced the highest percentage of growth (100%). Furthermore, acclimatization period only affected the percentage of growth, but had no effect on the growth components (number of tillers, leaves, and plant height). Plantlet that were acclimatization for 1 month have lower growth percentage compared with those that were acclimatized for 2 or 3 months.


Keywords


Kapolaga; Pemuliaan; Kultur jaringan; Rempah

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References


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DOI: http://dx.doi.org/10.21082/bullittro.v9n2.1994.72-75

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