Analisis Minyak Nilam Secara Kromatografi Lapis Tipis

NFN Hernani

Abstract


the thin-layer chromatography analysis of patchouli oil

Patchouly oil consist of various components, such as benzaldehyde, eugenol, cinamicaldehyde, azulene and sesquiterpene groups i.e: β patchoullene, α gauienene, α bulnesene and patchouli alcohol. In thin-layer chromatography analysis, its important to select the proper eluent, absorbant and detection solution, because they will affect the migration of components being analysed. To determine the components of patchouli oil by thin-layer chromatography, two kind of eluents with different polarities were tested. The system of solvent are ethyl acetate + hexane = 1 + 9 and the mixture of 5% ethyl acetate  + chloroform in hexane. Sulfuric acid 50% was used as adetection solution. From the analysis using two solvent systems, six group of sesquiterpenne can be identified from each solvent. To produce better separation of components, two dimensional technique should be developed.

Keywords


nilam; minyak atsiri; KLT; Pasca panen

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References


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DOI: http://dx.doi.org/10.21082/bullittro.v3n2.1988.89-92

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