Tri Lestari Mardiningsih, Rodiah Balfas


Essential oil, such as citronella, lemongrass, ageratum, and clove are known to be toxic and repel certain pests. Mixing two or more essential oils are expected increasing their insecticidal properties.  The experiments aimed to examine the effect of essential oils mixtures (EOs) on mortalities and oviposition deterrents of Crocidolomia pavonana and Helopeltis antonii. The experiments were conducted at the laboratory and green house of the Indonesian Spices and Medicinal Crops Research Institute. The tested mixtures were citronella and clove (CiC); lemongrass and clove (LC); ageratum and clove (AC); individual EO; control 1 (water + emulsifier); control 2 (water) at 0.5% concentration for all treatments. The ratio used were 1:1, 1:2, 1:4, 2:1 and 4:1. Parameters observed were the mortalities of C. pavonana larvae and H. antonii nymphs; and the ovipositions deterrents of both insects. The CiC and LC mixtures gave low mortality (<50%) to C. pavonana larvae, while AC at ratios 1:1, 1:2,1:4 less than 20%, AC 2:1 and 4:1 >60% and the individual ageratum oil caused 77.5% mortalities. The CiC 1:1 and LC (1:1, 1:2, and 4:1) showed >40% mortalities of     H. antonii, whereas the AC in all ratios enhanced the nymph mortalities. The number of eggs laid by C. pavonana at CiC and LC in all ratios were around 50-60% lower than individual EO. The mixture of AC 1:4 and 4:1 produced eggs nearly 50% lower than clove treatment. The CiC and LC at 1:1 produced the lowest numbers of egg laid by H. antonii, while AC 1:4 reduced 70% eggs laid compared to individual EO.


Crocidolomia pavonana; Helopeltis antonii; mixtures ratio

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Abo-El-Saad, M.M., Al Ajlan, A.M., Al-Eid, M.A. & Bou-Khowh, I.A. (2011) Repellent and Fumigant Effects of Essential Oil From Clove Buds Syzygium aromaticum L. against Tribolium castaneum (Herbest) (Coleoptera: Tenebrionidae). Journal of Agricultural Science and Technology A. 1, 613–620.

Autran, E.S., Neves, I.A., Da Silva, C.S.B., Santos, G.K.N., Da Câmara, C.A.G. & Navarro, D. (2009) Chemical Composition, Oviposition Deterrent and Larvicidal Activities Against Aedes aegypti of Essential Oils from Piper marginatum Jacq. (Piperaceae). Bioresource Technology. 100 (7), 2284–2288. doi:10.1016/j.biortech.2008.10.055.

Balfas, R. & Mardiningsih, T.L. (2016) Pengaruh Minyak Atsiri terhadap Mortalitas dan Penghambatan Peneluran Crocidolomia pavonana F. Bul Littro. 27 (1), 85–92.

Balfas, R. & Willis, M. (2009) Pengaruh Ekstrak Tanaman Obat terhadap Mortalitas dan Kelangsungan Hidup Spodoptera litura F. (Lepidoptera, Noctuidae). Bul Littro. 20 (2), 148–156.

Balfas, R., Willis, M. & Sugandi, E. (2013) Potential of Botanical Insecticides for Controlling Rhizome Fly (Mimegralla coeruleifrons Macquart) on Ginger.In: Rostiana,O. et al. (eds.) Proceedings of the International Seminar on Spices, Medicinal, and Aromatic Plants (SMAPs). Bogor, Indonesian Agency for Agricultural Research and Development (IAARD), pp.153–157.

Balittro (2008) Penyulingan Minyak Atsiri dengan SBCS-1000. Bogor, Balai Penelitian Tanaman Rempah dan Obat, pp.24–25.

Bhuiyan, M.N.I., Begum, J. & Akter, F. (2010) Constituents of The Essential Oil from Leaves and Buds of Clove (Syzigium caryophylatum (L.) Alston). African Journal of Plant Science. 4 (11), 451–454.

Cloyd, R.A., Galle, C.L., Keith, S.R., Kalscheur, N.A. & Kemp, K.E. (2009) Effect of Commercially Available Plant-Derived Essential Oil Products On Arthropod Pests. Journal of Economic Entomology. 102 (4), 1567–1579. doi:

Dadang, Fitriasari, E.D. & Prijono, D. (2009) Effectiveness of Two Botanical Insecticide Formulations to Two Major Cabbage Insect Pests on Field Application. J. ISSAAS. 15 (1), 42–51.

Dadang & Prijono, D. (2010) Insektisida Nabati: Prinsip, Pemanfaatan, dan Pengembangan. Bogor, Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor.

Isman, M.B. (2000) Plant Essential Oils for Pest and Disease Management. Crop Protection. 19 (8), 603–608.

Jaya, Singh, P., Prakash, B. & Dubey, N.K. (2014) Insecticidal Activity of Ageratum conyzoides L. Coleus aromaticus Benth. and Hyptis suaveolens (L.) Poit Essential Oils as Fumigant against Storage Grain Insect Tribolium castaneum Herbst. Journal of Food Science and Technology. 51 (9), 2210–2215. doi:10.1007/s13197-012-0698-8.

Karmawati, E. (2010) Pengendalian Hama Helopeltis spp. pada Tanaman Jambu Mete Berdasarkan Ekologi, Strategi dan Implementasinya. Pengembangan Inovasi Pertanian. 3 (2), 102–119.

Karmawati, E. & Mardiningsih, T.L. (2005) Hama Helopeltis spp. pada Jambu Mete dan Pengendaliannya. Perkembangan Teknologi Tanaman Rempah dan Obat. 17 (1), 1–6.

Khater, H.F. (2012) Prospects of Botanical Biopesticides in Insect Pest Management. Pharmacologia. 3 (12), 641–656. doi:10.5567/pharmacologia.2012.641.656.

Koul, O., Walia, S. & Dhaliwal, G.S. (2008) Essential Oils as Green Pesticides: Potential and Constraint. Biopestic. Int. 4 (1), 63–84.

Lima, R.K., Cardoso, M. das G., Moraes, J.C., Andrade, M.A., Melo, B.A. & Rodrigues, V.G. (2010) Chemical Characterization and Insecticidal Activity of the Essential Oil Leaves of Ageratum conyzoides L. on Fall Armyworm Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae). Bioscience Journal. 26 (1), 1–5.

Liu, X.C. & Liu, Z.L. (2014) Evaluation of Larvicidal Activity of the Essential Oil of Ageratum conyzoides L. Aerial Parts and Its Major Constituents against Aedes albopictus. Journal of Entomology and Zoology Studies. 2, 345–350.

Rattan, R.S. (2010) Mechanism of Action of Insecticidal Secondary Metabolites of Plant Origin. Crop Protection. 29 (9), 913–920.

Rohimatun & Laba, I.W. (2013) Efektivitas Insektisida Minyak Serai Wangi dan Cengkeh terhadap Hama Pengisap Buah Lada (Dasynus piperis China). Bul Littro. 24 (1), 26–34.

Setiawati, W., Murtiningsih, R. & Hasyim, A. (2011) Laboratory and Field Evaluation of Esential Oil from Cymbopogon nardus as Oviposition Deterrent and Ovicidal Activities against Helicoverpa armigera Hubner on Chili Pepper. Indonesian Journal of Agricultural Science. 12 (1), 9–16.

Siswanto, M.R., Omar, D. & Karmawati, E. (2008) Life Tables and Population Parameters of Helopeltis antonii (Hemiptera : Miridae) Reared on Cashew (Anacardium occidentale L.). Journal of Bioscience. 19 (1), 91–101.

Tajidin, N.E., Ahmad, S.H., Rosenani, A.B., Azimah, H. & Munirah, M. (2012) Chemical Composition and Citral Content in Lemongrass (Cymbopogon citratus) Essential Oil at the Three Maturity Stages. African Journal of Biotechnology. [Online] 11 (11), 2685–2693. Available from: doi:10.5897/AJB11.2939.

Tripathi, A.K., Upadhyay, S., Bhuiyan, M. & Bhattacharya, P.R. (2009) A Review on Prospects of Essential Oils as Biopesticides In Insect-Pest Management. Journal of Pharmacognosy and Phytotherapy. 1 (5), 52–63.

Willis, M., Laba, I.W. & Rohimatun (2013a) Efektivitas Insektisida Sitronellal, Eugenol dan Azadirachtin terhadap Hama Penggerek Buah Kakao (Conophomorpa cramerella) (Snell.). Bul Littro. 24 (1), 19–25.

Willis, M., Rohimatun, Laba, I.W. & Nurjanani (2013b) Control of Cocoa Pod Borer (Conopomorpha cramerella) and Cocoa Pod Sucker (Helopeltis sp.) using Essential Oil-Base Insecticides. In: Rostiana,O. et al. (eds.) Proceedings of the International Seminar on Spices Medicinal and Aromatic Plants (SMAPs). Bogor, Indonesia Agency for Agricultural Research and Development (IAARD), pp.115–120.

Wiratno (2008) Effectiveness and Safety of Botanical Pesticides Applied in Black Pepper (Piper Nigrum) Plantations. Wageningen University, Wageningen, the Netherlands.



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