EFEKTIVITAS ANTIFUNGI EKSTRAK ETANOL DAN MINYAK ATSIRI DAUN SIRIH (Piper betle) TERHADAP Trichophyton verrucosum SECARA IN VITRO

Djaenudin Gholib

Abstract


Daun sirih (Piper betle L.) diketahui mempunyai efek antifungi. Penelitian ini bertujuan untuk mengetahui efek daun sirih sebagai antifungi secara in vitro. Penelitian dilakukan di Laboratorium Mikologi, Balai Besar Penelitian Veteriner (BB Litvet) tahun 2012. Bahan penelitian adalah ekstrak etanol dan minyak atsiri. Fungi (cendawan) yang digunakan dalam pengujian ini adalah Trichophyton verrucosum, yaitu cendawan jenis kapang, penyebab penyakit kurap pada kulit (dermatofitosis) hewan dan manusia. Media yang digunakan, Sabouraud Dextrose Agar (SDA). Hasil penelitian ditentukan dengan perolehaan nilai konsentrasi hambat minimal (KHM). Bahan uji diencerkan dengan pengenceran ganda 6,250; 3,125; 1,56; 0,78; dan 0,39% untuk ekstrak etanol, dan 12,50; 6,250; 3,125; 1,56; 0,78; dan 0,39% untuk minyak atsiri. Koloni cendawan yang diuji ditumbuhkan pada media SDA dalam cawan petri selama 7 hari, masing-masing disuspensikan dengan aquades steril. Suspensi terdiri dari hifa dan sel spora. Kedua bahan yang diuji dituangkan ke dalam cawan petri steril dengan perbandingan 1:1, masing-masing 1 ml. Suspensi cendawan tanpa ekstrak atau minyak atsiri dituangkan ke dalam cawan petri sebagai kontrol negatif (0%). Media SDA yang masih cair dituangkan sebanyak 20 ml ke masing-masing petri. Inkubasi pada suhu 37oC selama 7 hari. Pengujian dilakukan dua kali ulangan. Hasil penelitian menunjukkan bahwa konsentrasi hambat minimal (KHM) ekstrak etanol adalah 1,56%, dan minyak atsiri 12,5%. Ekstrak etanol mempunyai efek antifungi yang lebih kuat dibandingkan dengan minyak atsiri.

Keywords


Piper betle; minyak atsiri; ekstrak etanol; Trichophyton verrucosum; KHM

Full Text:

PDF

References


Ali I, FG Khan, KA Suri, BD Gupta, NK Satti, P Dutt, F Afrin, GN Qazi, and IA Khan. 2010. In Vitro Antifungal Activity of Hydroxychavicol Isolated from Piper betle L. Annals of Clinical Microbiology and Antimicrobials 9(7): 1-9.

Arambewela LS, LD Arawwawala, and WD Ratnasooriya. 2005. Antidiabetic Activities of Aqueous and Ethanolic Extracts of Piper betle Leaves in Rats. Journal of Ethnopharmacology 102: 239-245. Bhalerao SA, DR Verma, RV Gavankar, NC Teli, YY Rane, VS Didwana, and A Trikannad. 2013.

Phytochemistry, Pharmacological Profile and Therapeutic Uses of Piper betle Linn. An Overview. Research and Reviews: Journal of Pharmacognosy and Phytochemistry 1(2): 10-19.

Bhattacharya S, D Banergee, AK Bauri, S Chattopadhyay, and SK Bandyopadhyay. 2007. Healing Properties of the Piper betle Phenol, Allypyrocatechol Against Indomethacin-Induced Stomach Ulceration and Mechanism of Action. World J. Gastroenterol 21: 3705-3713.

Chacraborty D and B Shah. 2011. Antimicrobial, Antioxidative and Antihemolytic Activity of Piper betle Leaf Extracts. Int. J. Pharm. Sci 3(3): 192-199.

Chahal J, O Renu, K Ajit, W Anu, and P Sidharth. 2011. Introduction, Phytochemistry, Traditional uses and Biological Activity of Genus Piper : A Review. International Journal of Current Pharmaceutical Review and Research (2)2: 130-144.

Cox SD, CM Mann, JL Markham, JE Gustafson, JR Warmington, and SG Wyllie. 2001. Determining the Antimicrobial Action of Tea Tree Oil. Molecules. 6: 87-91.

Gholib D dan S Rachmawati. 2010. Kapang Dermatofit Trichopphyton verrucosum Penyebab Penyakit Ringworm Pada Sapi. Wartazoa, Buletin Ilmu Peternakan dan Kesehatan Hewan Indonesia 1(20): 43-53.

Joung YH, HR Kim, MK Lee, and AJ Park. 2007. Fluconazole Susceptibility Testing of Candida Species by Flow Cytometry. J. Infect. 54: 504-508.

Nalina T and ZHA Rahim. 2006. Effect of Piper betle L. Leaf Extract on the Virulence Activity of Streptococcus mutans- An In Vitro Study. Pak. J. Biol. Sci 9: 1470-1475.

Nalina T and ZHA Rahim. 2007. The Crude Aqueous Extract of Piper betle L. and Its Antibacterial Effect Towards Streptococcus Mutans. Am. J. Biotechnol. Biochem 3: 10-15.

Ramani R, A Ramani, and SJ Wong. 1997. Rapid Flow Cytometry Susceptibility Testing of Candida albicans. J. Clinical Microbiol 35: 2320-2324.

Ramji N, R Iyer, and S Chandrasekaran. 2002. Phenolic Antibacterials from Piper betle in the Prevention of Halitosis. J. Ethnopharmacol 83: 149-152.

Sharma S, IA Khan, I Ali, F Ali, M Kumar, A Kumar, RK Johri, ST Abdullah, S Bani, A Pandey, KA Suri, BD Gupta, NK Satti, P Dutt, and GN Qazi. 2009. Evaluation of The Antimicrobial, Antioxidant and Anti-Inflammatory Activities of Hydroxychavicol for Its Potential Use as An Oral Care Agent. Antimicrobe Agents Chemoter 53: 216-222.

Shuster, S. 1999. Hydroxy-Pyridones as Antifungal Agents with Special Emphasis on Onychomycosis. Springer-Verlag Berlin Heidelberg. 109 p.

Singh M, S Shakya, VK Soni, A Dangi, N Kumar, and SM Battacharya. 2009. The N-Hexane and Chloroform Fractions of Piper betle L., Trigger Different Arms of Immune Responses in BALB/C Mice and Exhibit Antifilarial Activity Against Human Lymphatic Filarid Brugia Malay. Int. Immunopharmacol 2009. 9: 716-728.

Wirotesangthong M, I Naoki, dan T Hiroyuki. 2008. Inhibitory Effects of Piper betle on Production of Allergic Mediators by Bone Marrow-Derived Mast Cells and Lung Epithelial Cells. International Immunopharmacology 8: 453-457.




DOI: http://dx.doi.org/10.21082/bullittro.v25n2.2014.119-126

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Buletin Penelitian Tanaman Rempah dan Obat

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.



View My Stats

Creative Commons License
Buletin Penelitian Tanaman Rempah dan Obat is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Based on a work at http://ejurnal.litbang.pertanian.go.id/index.php/bultro

Buletin Penelitian Tanaman Rempah dan Obat (BUL LITTRO) Editorial Office :

Indonesian Spices and Medicinal Crops Research Institute (ISMCRI)
Jl. Tentara Pelajar No. 3 Bogor, Indonesia 16111
Telp. (0251) 8321879, Fax. (0251) 8327010
E mail : buletintro@gmail.com
Website : http://balittro.litbang.pertanian.go.id