TANGGAP PERTUMBUHAN DAN BIOMASA PEGAGAN (Centella asiatica (L.) Urban) PADA APLIKASI FUNGI MIKORIZA ARBUSKULA DAN PEMUPUKAN DI TANAH ANDOSOL

Budi Hartoyo, Octivia Trisilawati, Munif Ghulamahdi

Abstract


Penggunaan fungi mikoriza arbuskula (FMA) sebagai agensia hayati pada beberapa jenis tanaman saat ini mulai banyak mendapat perhatian. FMA diyakini berperan penting dalam meningkatkan pertumbuhan, perkembangan, produksi, dan mutu tanaman serta menghasilkan tanaman yang lebih tahan terhadap cekaman biotik dan abiotik.  Pupuk anorganik N, P, K digunakan sebagai sumber hara esensial pada budidaya pegagan (Centella asiatica). Penelitian ini bertujuan untuk mempelajari peran dan efektivitas FMA dan pemupukan terhadap pertumbuhan dan produksi biomasa tanaman pegagan pada tanah Andosol. Percobaan dilaksanakan dengan rancangan acak lengkap yang disusun secara faktorial, terdiri dari 2 faktor, diulang tiga kali. Faktor pertama ialah perlakuan FMA (inokulasi FMA dan tanpa FMA). Faktor kedua ialah dosis pupuk (5 taraf: tanpa pupuk sebagai kontrol, 25% dosis pupuk optimal, 50% dosis pupuk optimal, 75% dosis pupuk optimal, dan dosis optimal). Hasil penelitian menunjukkan bahwa perlakuan FMA nyata meningkatkan pertumbuhan dan produksi pegagan yaitu jumlah stolon primer 26,46%, jumlah buku 12,20% dan jumlah daun pada stolon primer terpanjang 17,94%, serta bobot kering akar 25,76%, terna 17,53% dan total 18,85%. Selain itu, pegagan yang bersimbiosis dengan FMA pada perlakuan tanpa pupuk menunjukkan serapan hara N, P, K lebih tinggi (N 24,4%; P 20,3% dan K 4,2%) dibandingkan tanpa FMA.

Keywords


Centella asiatica; fungi mikoriza arbuskula; pemupukan; pertumbuhan, biomas

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References


Abbott LK and AD Robson. 1984. The effect of mycorhizae on plant growth, Didalam : Powel CL. Dan Bagyraj DJ (Eds). Vesicular arbuscular mycorhizae. Florida:CRC Press. Inc. Boca Raton. pp. 113-130.

Akiyama K, K Matsuzaki and H Hayashi. 2005. Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi. Nature 435:824-827.

Anissa RF. 2006. Pengaruh pemberian ekstrak air daun pegagan (Centella asiatica) terhadap kemampuan kognitif dan kadar neuorotransmiter monoamine pada hipokampus tikus (Rattus novergicus L) galur Wistar jantan dewasa. Skripsi Sarjana Biologi. Program Studi Biologi. Sekolah Ilmu dan Teknologi Hayati. ITB. Bandung. 55 hlm.

Balai Penelitian Tanah. 2009. Analisis Kimia Tanah, Tanaman, Air dan Pupuk. Petunjuk Teknis. Edisi 2. BBSDL, Badan Litbang Pertanian, Deptan. 234 hlm.

Bermawie N, B Martono and S Purwiyanti. 2006. Description development and information documentation, Collaboration between Indonesian Medicinal Crops Research Institute, Indonesia Center Estate Crops Research and Development and International Plant Genetic Resources Institute. Unpublished. 40 hlm.

Berta G, A Trotta, A Fusconi, JE Hooker, M Munro, D Atkinson, M Giovanetti, S Morini, P Fortuna, B Tisserant, V Gianinazzi-Pearson and S Gianinazzi. 1995. Arbuscular mycorrhizal induced changes to plant growth and root system morphology in Prunus cerasifera. Tree Physiol 15: 281-293.

Bunpo P, K Kataoka, H Arimochi, H Natakayana, T Kuwahara, Y Bando, K Izumi, U Vinitketkumnuen and Y Ohnishi. 2004. Inhibitory effects of Centella asiatica on azoxymethane-induced aberrant crypt focus formation and carcinogenesis in the intestines of F344 rats. Food and Chemical Toxicology 42: 1987-1997.

Brundrett MC, N Melville and L Peterson. 1994. Practical methods in mycorrhiza research. Mycologue Publications. Ontario, Canada. 161 p.

Cardoso IM, TW Kuyper. 2006. Mycorrhizas and tropical soil fertility. Agriculture, Ecosystems and Environment 116: 72-84.

Chaurasia B, A Pandey and LMS Palni. 2005. Distribution, colonization and diversity of arbuscular mcorrhizal fungi associated with central Himalayan rhododendrons. Forest Ecol Manage 207: 315-324.

Cuenca G, DZ Andrade and E Meneses. 2001. The presence of aluminium in arbuscular mycorrhizas of Clusia multifora exposed to increased acidity. Plant and Soil 231: 233-241.

De La Cruz RE, MQ Manalo, NS Aggangan and JD Tambalo. 1988. Growth of three legume trees inoculated with VA mycorrhizal fungi and rhizobium. Plant Soil. 108: 111-115.

Delvian. 2003. Keanekaragaman cendawan mikoriza arbuskula (CMA) di hutan pantai dan potensi pemanfaatannya. [Disertasi]. Program Pascasarjana. IPB. Bogor. 159 hlm.

Douds DD Jr and NC Johnson. 2007. Contributions of arbuscular mycorrhizas to soil biological fertility. Di dalam: Abbott LK, Murphy DV. (editor). Soil Biological Fertility – A Key to sustainable land use in agriculture. New York: Springer Science Business Media. pp. 129-162.

Ghulamahdi M, SA Aziz, N Bermawie dan O Trisilawati, 2008. Studi penyiapan standar operasional prosedur budidaya untuk produksi bioaktif mendukung standarisasi mutu pegagan. Laporan Hasil Penelitian KKP3T. 71 hlm.

Ghulamahdi M, SA Aziz, N Bermawie dan O Trisilawati, 2009. Studi Penyiapan Standar Operasional Prosedur Budidaya untuk Produksi Bioaktif Mendukung Standarisasi Mutu Pegagan. Laporan Hasil Penelitian KKP3T. 98 hlm.

Hanum C. 2004. Penapisan beberapa galur kedelai (Glycine max. Merr.) toleran cekaman aluminium dan kekeringan serta tanggap mikoriza vesikular arbuskular [Disertasi]. Sekolah Pascasarjana IPB, Bogor. 162 hlm.

Hartoyo B, M Ghulamahdi, LK Darusman, ZA Aziz dan I Mansur. 2011. Keanekaragaman fungi mikoriza arbuskula (FMA) pada rizosfer tanaman pegagan (Centella asiatica L. Urban). Jurnal Penelitian Tanaman Industri 17(1): 32-40.

Haryantini BA dan HM Santoso. 2006. Pertumbuhan dan hasil cabai merah (Capsicum annum) pada andisol yang diberi mikoriza, pupuk fosfor dan zat pengatur tumbuh. http://72.14.235.104/ search?q =cache:Gut_ m4d67ZgJ :images.soemarno. multiply.com/. 11 p.

Heyne K. 1987. Tumbuhan Berguna Indonesia Jilid III, Badan Penelitian dan Pengembangan Kehutanan, Departemen Kehutanan, Jakarta: 1.884 hlm.

Hong SS, JH Kim, H Li and CK Shim. 2005. Advanced Formulation and Pharmacological Activity of Hydrogel of the Titrated Extract of C. asiatica. Arch Pharm Res. 28(4): 502-508.

Hsu YL, PL Kuo, LT Lin and CC Lin. 2005. Asiatic acid, a triterpene, Induces apoptosis and cell cycle arrest through activation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways in human breast cancer cells. The Journal of Pharmacology and Experimental Therapeutics 313(1): 333-344.

IPB (Institut Pertanian Bogor). 2005. Pasar domestik dan ekspor produk tanaman obat. Pusat Studi Biofarmaka Institut Pertanian Bogor. 3 hlm.

Januwati M dan M Yusron. 2005. Budidaya tanaman pegagan. Sirkuler No. 11. Balai Penelitian Tanaman Obat dan Aromatika, Bogor. 4 hlm.

Jeong BS. 2006. Structure-activity relationship study of asiatic acid derivatives for new wound healing agent. Arch Pharm Res 29(7): 556-562.

Kabirun S. 2002. Tanggap padi gogo terhadap inokulasi jamur mikoriza arbuskula dan pemupukan fosfat di entisol. Jurusan Tanah, Fakultas Pertanian, Universitas Gadjah Mada Yogyakarta. Jurnal Ilmu Tanah dan Lingkungan 3(2): 49-56.

Kartika E. 2006. Tanggap pertumbuhan, serapan hara dan karakter morfofisiologi terhadap cekaman kekeringan pada bibit kelapa sawit yang bersimbiosis dengan CMA. [Disertasi]. Sekolah Pascasarjana IPB. Bogor. 188 hlm.

Kumar VMH and YK Gupta. 2002. Effect of different extracts of Centella asiatica on cognition and markers of oxidative stress in rats. J Ethnopharmacol 79(2): 253-60.

_____________________. 2003. Effect of Centella asiatica on cognition and oxidative stress in an intracerebroventricular streptozotocin model of Alzheimer's disease in rats: Clin Exp Pharmacol Physiol 30(5-6): 336-42.

Li T and ZW Zhao. 2005. Arbuscular mycorrhizas in a hot and ecosystem in Southwest China. Appl Soil Ecol 29: 135-141.

Lynch JP and KM Brown. 2001. Topsoil foraging: an architectural adaptation of plants to low phosphorus availability. Plant Soil 237: 225-237.

Mathew G, L Joseph and Ramaswamy. 2009. Anti-allergic, anti-pruritic, and anti-inflammatory activities of Centella asiatica ekstracts. Afr. J. Trad. CAM 6(4): 554-559.

Mathur A, AK Mathur, SYadav and P Verma. 2007. Centella asiatica (L.) Urban-status and scope for commercial cultivation. J. Med. Arom. Plant Sci 129: 151-162.

Miransari M, HA Bahrami, F Rejali and MJ Malakouti. 2009. Effects of arbuscular mycorrhiza, soil sterilization, and soil compaction on wheat (Triticum aestivum L.) nutrients uptake. Soil Till. Res. 104: 48-55.

Musyarofah N, S Susanto, SA Aziz dan S Kartosoewarno. 2007. Respon tanaman pegagan (Centella asiatica L. Urban) terhadap pemberian pupuk alami di bawah naungan. Bul Agron 35: 217-224.

Nusantara AD. 2011. Pengembangan produksi inokulan fungi mikoriza arbuskula berbasis bahan alami dan pemanfaatannya untuk produksi bibit jati (Tectona grandis L.f). [Disertasi]. Sekolah Pascasarjana, Institut Pertanian Bogor. 186 hlm.

Ortas I. 2010. Effect of mycorrhiza application on plant growth and nutrient uptake in cucumber production under field conditions. Spanish Journal of Agricultural Research 8: S116-S122.

Ortas I, N Sari, C Akpinar and H Yetisir. 2011. Screening mycorrhiza species for plant growth, P and Zn uptake in pepper seedling grown under greenhouse conditions Scientia Horticulturae 128: 92-98.

Ouahmane L, J Thioulouse, M Hafidi, Y Prin, M Ducousso, A Galiana, C Plenchette, M Kisa and R Duponnois. 2007. Soil functional diversity and P solubilization from rock phosphate after inoculation with native or allochtonous arbuscular mycorrhizal fungi Forest Ecology and Management 241: 200-208.

Porras-Soriano A, ML Soriano-Martín, AA Porras-Piedra and R Azcón. 2009. Arbuscular mycorrhizal fungi increased growth, nutrient uptake and tolerance to salinity in olive trees under nursery conditions. J. Plant Physiol 166: 1350-1359.

Read DJ, J Perez-Moreno. 2003. Mycorrhizas and nutrient cycling in ecosystems–a journey towards relevance. New Phytologist 157: 475-492.

Rhodes LH, JW Gerdemann. 1978. Translocation of calcium and phosphate by external hyphae of vesicular arbuscular mycorrhizae. Soil Sci 126: 125-126.

Sasli I, S Yahya, Sudradjat, Y Setiadi dan Sudarsono. 2008. Perbaikan pertumbuhan dan kualitas tanaman lidah buaya di tanah gambut dengan aplikasi mikoriza arbuskula dan pemupukan. Bul. Agron 36(3): 248-254.

Setiadi Y. 1996. The practical application of arbuscular mycorrhizal fungi for enhancing tree establishment in degraded nickel mine sites at PT INCO Soroako. Paper presented on IUFRO International Symposium on Accelerating Natural Succession of Degraded Tropical Lands. 11-13 June 1996. Washington DC.

Setiadi Y. 2001. Arbuscular Mycorrhizal Inoculum Production for Reforestation in Indonesia. Proceedings of International Workshop BIOREFOR. Tokyo, Japan. 7-11 October 2001. pp. 102-107

Sieverding E. 1991. Vesicular-arbuscular mycorrhiza management in tropical agroecosystems. Bremen, Germany: Deutsche Gesellschaft fur Technische Zusammenarbeit.

Smith SE dan DJ Read. 1997. Mycorrhizal symbiosis. Second edition. Harcourt Brace and Company Publisher. pp. 34-160.

__________________. 2008. Mycorrhizal symbiosis. Third edition: Academic Press. Elsevier Ltd. New York, London, Burlington, San Diego. 768 p.

Subramanian KS, V Tenshia, K Jayalakshmi and V Ramachandran. 2009. Biochemical changes and zinc fractions in arbuscular mycorrhizal fungus (Glomus intraradices) inoculated and uninoculated soils under differential zinc fertilization. Appl. Soil Eco. 43: 32–39.

Sutardi. 2008. Kajian waktu panen dan pemupukan fosfor terhadap pertumbuhan dan produksi asiatikosida tanaman pegagan (Centella asiatica L. Urban) di dataran tinggi. [Tesis]. Sekolah Pascasarjana IPB. Bogor. 82 hlm.

Swasono DH. 2006. Peranan mikoriza arbuskula dalam mekanisme adaptasi beberapa varietas bawang merah terhadap cekaman kekeringan di tanah pasir pantai. [Disertasi]. Sekolah Pascasarjana, Institut Pertanian Bogor. 160 hlm

Syarif AA. 2007. Adaptasi dan Ketenggangan genotipe padi terhadap defisiensi fosfor di tanah sawah. [Disertasi]. Sekolah Pascasarjana IPB. Bogor.131 hlm.

Taiwo LB and AA Adegbite. 2001. Effect of arbuscular mycorrhizal and Braadyrhizzobium inoculums on growth, N2 fixation and yield of promiscuously modulating soybean (Glycine max). Moor J. Agri. Res 2: 110-118.

Trisilawati O. 2005. Respon Tiga Klon Kumis Kucing (Orthosipon aristatus) terhadap Mikoriza Arbuskula. Bul. Littro 16(1): 18-26.

__________. 2007. Efektivitas mikoriza arbuskula dan pupuk NPK terhadap pertumbuhan dan produksi nilam (Pogostemon cablin Benth.). Prosiding Konggres Nasional Mikoriza Indonesia II. Bogor, 7-9 Juli 2007. Hlm. 45-51.

__________. 2010. Pengaruh pupuk organic dan anorganik terhadap pertumbuhan dan produksi nilam varietas Tapaktuan. Prosiding Konferensi Nasional Minyak Atsiri. Bandung 20-21 Oktober 2010. 75-82.

Wang XS and JN Liu L. 2005. Immunological activities and structure of pectin from Centella asiatica. Carbohydrate Polymers 60: 95-101.

Wang B and YL Qui. 2006. Phylogenetic distribution and evolution of mycorrhizas in land plants. Mycorrhiza 16: 299-363.

Winarto WP dan M Surbakti. 2003. Khasiat dan manfaat pegagan. Tanaman penambah daya ingat. Agromedia Pustaka, Jakarta. 64 hlm.

Xiao TJ, QS Yan, W Ran, GH Xu, QR Shen. 2010. Effect of inoculation with arbuscular mycorrhizal fungus on nitrogen and phosphorus utilization in upland rice-mungbean intercropping system. Agric. Sci. China 9: 528-535.

Zainol MK, A Abd-Hamid, S Yusof and R Muse. 2003. Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L.) Urban. Food Chemistry 81: 575-581.

Zhang LN, JJ Zheng, L Zhang, X Gong, H Huang, CD Wang, B Wang, MJ Wu, XH Li, WJ Sun, YJ Liu and JY Wan. 2011. Protective effect of asiaticoside on septic lung injury in mice. Experiment and Toxicologic Pathology 63: 519-525.

Zhu YG, TR Cavagnaro, SE Smith and S Dickson. 2001. Backseat driving. Accessing phosphate beyond the rhizosphere-depletion zone. Trends Plant Sci 6: 194-195.




DOI: http://dx.doi.org/10.21082/bullittro.v26n2.2015.87-98

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