Rita Noveriza, Mesak Tombe, H. Rialdy, Dyah Manohara


Application of Fusarium oxysporum Non Pathogenic (FoNP) in Inducing Resistance of Black Pepper Seedlings to Phytophthora capsici

Phytophthora capsici Leon is a soil borne pathogen which is known as the causal agent of Foot rot disease of black pepper (Piper nigrum L.). Induced plant resistance against pathogens is a widespread phenomenon that has been intensively investigated with respect to the underlying signaling pathway as well as to its potential use in plant protection. This study used non pathogenic Fusarium oxysporum (FoNP) For inducing resistance on black pepper cuttings against Foot rot disease at laboratory and glass house of Phytopatology Laboratory of Indonesian Spice and Medicinal Crop Research Institute-Bogor from July until December 2004. It was observed that FoNP had ability to reduce disease severity. The level of  effectiveness was 84,99 percent (at Four months seedlings). The level of effectiveness of fungicide treatment was 14,49 percent. FoNP was able to colonize black pepper seedlings up to two and a half months. The lowest viabilities of P. capsici was observed on black pepper seedling treated with Organo-TRIBA. This study suggest that FoNP has potential to be used in inducing resistace of black pepper seedlings to foot rot disease, eventually will reduce severity of the disease.



Induce resistance; black pepper; Phytophthora capsici; Non pathogenic Fusarium oxysporum

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Anith, K.N., N.V. Radhakrishnan and T.P. Manomohandas, 2002. Mana- gement of nursery wilt of black pepper (Piper nigrum L.) with antagonistic bacteria. Current Science 83(5):561-562.

Benhamou, N. and C. Garand, 2001. Cytological analysis of defense related mechanisms induced in pea root tissues in response to colonization by the nonpathogenic Fusarium oxysporum strain Fo47. Phytopathology 91:730-740.

Benhamou, N., C. Garand and A. Goulet, 2002. Ability of nonpatho- genic Fusarium oxysporum Starin Fo47 to induce resistance against Pythium ultimum infection in cucumber. Applied and Environ-mental Microbiology 68 (8) :4044-4060.

Duijff, B.J., D. Poulain, C. Olivain, C.Alabouvette and P. Lemanceau,

Implication of systemic induced resistance in the sup- pression of fusarium wilt of tomato by Pseudomonas fluorescens WCS417r and by nonpathogenic Fusarium oxysporum Fo47. Eur. J. Plant Pathol. 104:903-910.

Eparvier, A. and C. Alabouvette, 1994. Use of ELISA and GUS- transformed strains to study competition between pathogenic and non-pathogenic Fusarium oxysporum for root colonization. Biocontrol Sci. Technol. 4:35-47.

Fuchs, J.G., Y. Moenne-loccoz and G. Defago, 1997. Non pathogenic Fusarium oxysporum Fo.47 induces resistance to Fusarium wilts in tomato. Plant Disease 81:492-496.

Hoitink, H.A.J. and M.J. Boehm, 1999. Control within the content of soil microbial communities: A Substrate- dependent phenomenon. Ann. Rev. of Phytopathol. 37:427-446.

anthracnose in cucumber due to plant growth-promoting fungi and studies on mechanism. See Ref. 72a, pp.164-169.

Kasim, R., 1981. Ketahanan tujuh species lada terhadap

Phytophthora. Pembr. Littri 8 (39):34-38.

Kuc, J., 1982. Induced immunity to plant disease. Bioscience 32:854-860.

Kuc, J., 1995. Induced systemic resistance- an overview. See Ref.

, pp 169-175.

Krause, M.S., T.J.J. De Ceuster, S.M. Tiquia, F.C. Michel Jr., L.V. Madden and H.A.J. Hoitink, 2003. Isolation and characterization of rhizobacteria from composts that suppress the severity of bacterial leaf spot of radish. Phytopathology 93:1292-1300.

Labrie,C., P. Leclerc, N. Cote, S. Roy;R. Brzezinski, R. Hogue and C. Beaulieu, 2001. Effect of chitin waste-based composts produced by two-phase composting on two oomycete plant pathogens. Plant Soil 235:27-34.

Manohara, D., R. Kasim and D. Sitepu,1992. Current research status of foot rot disease in Indonesia. Proceedings of the International Workshop on Black Pepper Diseases. Bandar Lampung- Indonesia, 3-5 December 1991. pp.144-154.International Agricultural Research. Pp:132-135.

Manohara, D., K. Mulya and D. Wahyuno, 2004b. Phytophthora disease on black pepper and the control measures. Journal of the Pepper Industry 1(1):37-49.

Ownley, B.H., D.M. Benson and T.E. Bilderback, .1990. Physical properties of container media and relation to severity of Phytophthora root rot of Rhododendron. J.Am.Soc.Hortic.Sci.115:564-570.

Pharand, B., O. Carisse and N. Benhamou, 2002. Cytological aspects of compost-mediated induced resistance against Fusarium crown and root rot in tomato. Phytopathology 92:424-

Ristaino, J.B. and S.A. Johnston, 1999. Ecologically based approaches to management of Phytophthora blight on bell pepper. Plant Dis.


Tsao, P.H. and A. Alizadeh, 1988. Recent advances the taxonomy and nomenclature of the so-called “Phytophthora palmivora”MF4 occuring on cocoa and other tropical crops. Pages 441-445


Manohara, D., K. Mulya, A.in:10 Int. Cocoa Res. Conf. Proc.

Purwantara and D. Wahyuno, 2004a. Phytophthora capsici on black pepper in Indonesia. In:Diversity and Management of Phytophthora in South East Asia (Eds.)Andre Drenth and David I. Guest. Australian Center for Santo Domingo.17-23 May, 1987.

Tombe, M, D. Sitepu dan S. Mogi,1997. Present status of biological control research of vanilla stem rot disease in Indonesia. Proceeding of the Fourth International Workshop on Plant Growth Promotion

Rhizobacteria. Japan-OECD. Workshop. p13-17.

Tombe, M., Sukamto, E. Taufik dan Zulhisnain, 1999. Penggunaan Fusarium oxysporum non patogenik (FoNP) untuk memperoleh bibit panili yang bebas patogen BBV. Prosidung Simposium Hasil Penelitian dan Pengembangan Tanaman Perke- bunan. p152-159.

Tombe, M., Zulhisnain dan E. Taufik, 2001. Penggunaan Bio-FOB strain

-AM untuk pengendalian penyakit BBP panili secara hayati. Prosiding Simposium Rempah Indonesia. Jakarta, 13-14 September 2001. p.209-216.

Tombe, M., D. Wahyuno dan Purnayasa, 2003. Paket teknologi pengendalian penyakit JAP jambu mente. Materi dibawakan pada sosialisasi hasil penelitian Proyek PHT Perkebunan Rakyat di tingkat Pemandu SLPHT, Mataram 17-18 Desember 2003.

Whipps, J.M., 2001. Microbial interactions and biocontrol in the rhizosphere. Journal of Experimental Botany


Yamaguchi, K., M. Kida, M. Arita and M. Takahashi, 1992. Induction of systemic resistance by Fusarium oxysporum MT0062 in solanaceous crops. Ann. Phytopathology Soc. Japan 58:16-22.

You, M.P. and K. Sivasithamparam,1995. Changes in microbial population of an avocado plantation mulch suppressive of Phytophthora cinnamomi. Appl. Soil Ecol. 2:33-43.

Zhang,W., W.A. Dick and H.A.J.Hoitink, 1996. Compost-induced systemic acquired resistance in cucumber to Pythium root rot and anthracnose. Phytopathology 86 : 1066 - 1070.

Zhang, W., D.Y. Han;W.A. Dick;K.R.

Davis and H.A.J. Hoitink, 1998. Compost and compost water extract-induced systemic acquired resistance in cucumber and arabidopsis. Phytopathology 88 : 450 - 455.

DOI: http://dx.doi.org/10.21082/bullittro.v16n1.2005.%25p


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