PENGARUH PENURUNAN UNSUR MAKRO DAN PEMBERIAN ABSISIC ACID TERHADAP MULTIPLIKASI TUNAS TAPAK DARA (Vinca rosea) SECARA IN VITRO

Natalini Natalini, Kristina Kristina

Abstract


Effects of reduced–macro nutrients, and ABA to shoots multiplication of periwinkle (Vinca rosea) in vitro

Research regarding effect of reduced-macro nutrients and ABA to shoot multi-plication of periwinkle (Vinca rosea) in vitro   has been performed in the Laboratory of Germplasm and Breeding, Indonesian Spice and Medicinal Crops Research Institute, from April 2003 to March 2004. The culture media applied were  MS, ¾  and ½  MS +  (20 and 30) g/l sucrose + BA 0,1  mg/l and maintained growth inhibitor  ABA (1 and 2) mg/l. The experiment was design as a completely randomized with 10 replicated. Results showed that there was no significant different on the growth of shoots from all of MS medium until  culture 9 months with grow up to 90%. Addition ABA 1 and 2 mg/l in media,  made browning shoots until 7 months culture periods, and growing ability of 40 - 60%. Result from test of  shoots regeneration showed,  that MS + BA 0,1 mg/l + sucrose 30 g/l  (control) were the best medium for  conservation  with the highest number of shoots 8,4.


Keywords


Vinca rosea; in vitro; conservation; macro nutrient; ABA

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References


Babu, K.N.; S.P. Geetha, D. Minoo, P.N. Ravindran and K. V. Peter,

In vitro conservation of cardamon (Elletaria cardamomum Maton) germplasm. Plant Genetic Resources Newsletter. No. 119 :41-45. IPGRI.

Bermawie, N.; S.F. Syahid; O.Rostiana; L. Udarno; E. Hadipoentyanti; D. Seswita, S. Wahyuni, Hobir dan Hadad, 2003. Konservasi, Evaluasi dan Doku- mentasi Plasma Nutfah Tanaman Rempah dan Obat. Laporan Akhir Tahun Balittro. Badan Litbang Departemen Pertanian. 79 h.

Bonnier, F.J.M. and J.M. van Tuyl,1997. Long term in vitro storage of lily: Effect of temperature and conservation of nutrient and sucrose. Plant Cell, Tissue and Organ Culture. 49: 81-87.

Dalimartha, S., 1997. Atlas Tumbuhan Obat Indonesia. Jilid 1. Trubus Agriwidya. h. 10.

Gadner, F.P., R.B. Pearce and R.L.Mitchell, 1991. Fisiologi Tumbuhan Budidaya. Univ. Indonesia. Jakarta. 428 p.

Gati, E. dan I. Mariska, 1997. Kultur in vitro sebagai metode pelestarian tumbuhan obat langka. Buletin Plasma Nutfah II (1): 1-8.

Herwinia, M., 1993. Pengaruh media padat dan cair serta penambahan kombinasi benzil adenin (BA) dengan adenis sulfat, air kelapa dan arang aktif terhadap organogenesis jaringan tanaman jahe (Zingiber officinale Rosc.). Skripsi FMIPA- UNPAD, Bandung. 64 h.

Hoesen, D.S.H., 2000. Penyimpanan plasma nutfah Musa spp kultivar

Ambon, Raja dan Tanduk secara in vitro dalam medium pertumbuham minimal. Prosiding Simposium Nasional Pengelolaan Pemuliaan dan Plasma Nutfah Ilmu Pengetahuan dan Teknologi. Buku2. Bogor 22-23 Agustus. Kerjasama PERIPI-Badan Litbang Pertanian-Dirjen Perkebunan dan KNPN. hal.333-341.

Kristina, N.N., D. Seswita dan A.Husni, 1995. Penyimpanan dan regenerasi tanaman obat inggu melalui kultur in vitro. Prosiding Evaluasi Hasil Penelitian Tanaman Industri. Puslitbangtri. Bogor. II:25-33.

Kristina, N.N dan N. Bermawie, 1999.Pengaruh subkultur dan lama periode kultur pada daya multiplikasi tunas lada (Piper nigrum L.) asal biji varietas Petaling 1. Jurnal Litantri 5(3):98-102.

Nyman, L., C.J. Gonzales and J.Arditti, 1984. Salt tolerance tissue of potato :selection and constituents. In symposium of International Potato Centre (CIP), p. 132-142.

Salisbury, F. B. and C. W. Ross, 1992.Fisiologi Tumbuhan Jilid 3. Hak Cipta Edisi Bahasa Indonesia. Penerbit ITB Bandung. 343 hal.

Sumarsi dan T.F. Hutajulu, 2003.Isolasi dan analisis vinblastin dan vincristine dari tanaman tapak dara (Catharanthus L.) berdasarkan jarak potong tanam. Ed. Agus

Purwanggana dkk. Prosiding Seminar Nasional Tumbuhan Obat Indonesia XXIII, Jakarta, 25-26 Maret. II. 403-407.

Syahid, S.F. dan N. Bermawie, 2000.Pengaruh pengenceran media dasar terhadap pertumbuhan kultur jahe dalam penyimpanan secara in vitro. Jurnal Litantri V (4):115-118.

Seswita, D., Amalia dan E.Hadipoentyanti, 2003. Konservasi in vitro panili (Vanilla planifolia Andrews) melalui pertumbuhan minimal. Buletin Littro. Vol XIV (1): 1-7.

van deHouwe, I. D. De Smet., H.Tezenas du Monteel and R. Swennen, 1995. Variability in storage potential of banana shoot culture under medium term storage condition. Plant Cell, Tissue and Organ Culture. 42:269-274.

Withers, L.A., 1983. Germplasm storage. In. S.H. Manthell and H. Smith (Ed.). Plant Biotechnology Cambridge University. Press London. 187-218




DOI: http://dx.doi.org/10.21082/bullittro.v16n1.2005.%25p

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