Antioxidant Activity of Kecibeling, Red Mangrove, and Star Gooseberry at Different Extraction Methods and Extract Ratios

Lilik Sulastri, Ika Oktavia, Partomuan Simanjuntak

Abstract


Indonesian medicinal plants, such as Strobilanthes crispa (L.) Blume (locally known as kecibeling), red mangrove (Rhizophora stylosa Griff.) and star gooseberry {Sauropus androgynus (L.) Merr.} contain active compounds that act as antioxidants. The study aimed to determine the effect of extraction methods (maceration and infusion) on the antioxidant activity of both single extract and  several combination ratios of the extracts mixture of kecibeling leaves, red mangrove leaves and star gooseberry stems. Dried powdered of the samples of a 40 mesh size were extracted using 96 % ethanol solvent (maceration method) and water solvent (infusion method). A single extract or a combination of the three-single extracts (1: 1: 1; 1: 1: 2; 1: 2: 1; and 2: 1: 1) were then evaluated for their antioxidant activity based on the free radical method 2,2-diphenyl-1-picrylhydrazyl (DPPH). In general, extraction method using ethanol (maceration) was better than water (infusion). Antioxidants activities from ethanol extracts of the kecibeling leaves showed the strongest activity with an inhibitory concentration (IC50) value of 37.65 ppm than the infusion extracts. The combination of a single ethanol extract from the three plants at a ratio of 2: 1: 1 was synergistic, which indicated by its strongest antioxidant activity (IC50 = 18.78 ppm).  However, it still below the antioxidant activity of vitamin C (IC50 = 4.24 ppm). Ethanol extract of kecibeling leaves singly or combined with the ethanol extracts of red mangrove leaves and star gooseberry stems can be developed as a potential antioxidant.

Keywords


Rhizophora stylosa; Sauropus androgynus; Strobilanthes crispa; infusion; maceration

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References


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DOI: http://dx.doi.org/10.21082/bullittro.v31n1.2020.1-7

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