The Compatibility of Five Superior Cacao Clones As Scions with Rootstock Of Half-Sib Family of Sulawesi 01 Clone

Dibyo Pranowo, Edi Wardiana


Grafting is the most common cocoa propagation technique applied by farmers. One of the factors that determine the grafting success in cacao is the compatibility level between the scion and rootstock. The objectives of this research was to evaluate the grafting compatibility of half-sib family of Sulawesi 01 clone as rootstock with five superior cacao clones  i.e. Sulawesi 01, Sulawesi 02, Sca 6, MCC 01, and MCC 02 as scions. The research was conducted at Pakuwon Experimental Station, West Java, at the altitude of 450 m above sea level with Latosol type of soil and B type of climate (Schmidt & Fergusson), from April to September 2015. This research used the randomized completely block design with five treatments of grafting combinations and five replications. Observation was taken at 14th, 21st, and 28th days after grafting on the total percentage of surviving graftings, percentage of sprouting grafting, percentage of grafting that have not sprouted, and bud sprouting rates.  Data were analyzed by variance, correlation, and regression analysis. The results showed that the surviving grafting, the sprouting grafting and the rate of sprouting up to the 28th  days after grafting varied among the scions. Based on the parameters observed, Sulawesi 01, Sulawesi 2, and Sca 6 demonstrated higher compatibility rate compared to MCC 01 and MCC 02. The results is applicable in seedling provision through grafting techniques in order to support cacao rejuvenation and or rehabilitation.


Cacao; grafting; compatibility

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Andrews, P.K., & Marquez, C.S. (1993). Graft incompatibility. Horticultural Reviews, 15, 183–232.

Anita-Sari, I., & Susilo, A.W. (2012). Keberhasilan sambungan pada beberapa jenis batang atas dan famil batang bawah kakao (Theobroma cocoa L.). Pelita Perkebunan, 28(2), 72–81.

Baon, J.B. (2011). 100 Tahun Pusat Penelitian Kopi dan Kakao Indonesia 1911-2011 (p. 373). Jember: Pusat Penelitian Kopi dan Kakao Indonesia.

Bhuiyan, M.F.A., Rahim, M.A., & Alam, M.S. (2010). Study on the growth of plants produced by epicotyl (stone) grafting with different rootstock-scion combinations in mango. J. Agrofor. Environ., 3(2), 163–166.

Chipojola, F.M., Mwase, W.F., Kwapata, M.B., Njoloma, J.P., Bokosi, J.M., & Maliro, M.F. (2013). Effect of tree age, scion source and grafting period on the grafting success of cashew nut (Anacardium occidentale L.). Afric. J. of Agric. Res., 8(46), 5785–5790.

Darikova, J.A., Savva, Y.V., Vaganov, E.A., Grachev, A.M., & Kuznetsova, G.V. (2011). Graft of woody plants and the problem of incompatibility between scion and rootstock (a review). Journal of Siberian Federal University, Biology, 1(4), 54–63.

Direktorat Jenderal Perkebunan. (2013). Klon kakao tahan organisme pengganggu tumbuhan (OPT). Jakarta: Direktorat Jenderal Perkebunan, Kementerian Pertanian RI.

Errera, P. (1998). Implications of phenolic compounds in graft incompatibility in fruit tree species. Scientia Horticulturae, 74(3), 195–205.

Flaishman, M.A., Loginovsky, K., Golobowich, S., & Lev-Yadun, S. (2008). Arabidopsis thaliana as model system for graft union development in homografts and heterografts. J. Plant Growth Regul., 27, 231–239.

Gainza, F., Opazo, I., & Muńoz, C. (2015). Graft incompatibility in plants: Metabolic changes during formation and establishment of the rootstock/scion union with emphasis on Prunus species. Chilean J. of Agric. Res., 75(suppl. 1), 28–34.

Goldschmidt, E.E. (2014). Plant grafting: New mechanisms, evolutionary implications. Plant Science, 5(727), 1–9.

Gȕçlȕ, S.F., & Koyuncu, F. (2012). A method for prediction of graft incompatibility in Sweet Cherry. Not. Bot. Horti .Agrobo., 40(1), 243–246.

Hamdan, A-J.S., & Basheer-Salimia, R. (2010). Preliminary compatibility between some Table-Gravepine scion and Phylloxera-Resistant rootstock cultivars. Jordan J. of Agric. Sci., 6(1), 1–10.

Hartmann, H.T., Kester, D.E., Davies, F.T., & Geneve, R.L. (2010). Plant propagation: Principles and practices. In Chapter 11, Principles of grafting and budding (pp. 415–463). 7th edition. Pearson Education, Prentice Hall, Upper Saddle River, NJ.

Hossein, D.G., Farajollah, S., & Hassanpour, H. (2008). Identification of graft incompatibility of Pear cultivar on Quince rootstock by using isozymes banding pattern and starch. Asian J. of Plant Sci., 7(1), 109–112.

Kementerian Pertanian. (2008a). Pelepasan kakao klon Sulawesi 01 sebagai varietas unggul. SK Mentan No. 1694/Kpts/ SR.120/12/2008. Jakarta: Kementerian Pertanian RI.

Kementerian Pertanian. (2008b). Pelepasan kakao klon Sulawesi 02 sebagai varietas unggul. SK Mentan No. 1695/Kpts/ SR.120/12/2008. Jakarta: Kementerian Pertanian RI.

Kementerian Pertanian. (2009). Pelepasan kakao klon Sca 6 sebagai varietas unggul. SK Menteri Pertanian No. 1984/Kpts/SR.120/4/2009. Jakarta: Kementerian Pertanian RI.

Kementerian Pertanian. (2014a). Pelepasan kakao klon M01 sebagai varietas unggul dengan nama MCC 01. SK Menteri Pertanian No. 1083/Kpts/SR.120/ 10/2014. Jakarta: Kementerian Pertanian RI.

Kementerian Pertanian. (2014b). Pelepasan kakao klon M45 sebagai varietas unggul dengan nama MCC 02. SK Menteri Pertanian No. 1082/Kpts/SR.120/ 10/2014. Jakarta: Kementerian Pertanian RI.

Kohatsu, D.S., Zucareli, V., Brambilla, W.P., Ono, E.O., da Silva, T.R.B., & Rodrigues, J.D. (2013). Peroxidase and polyphenol oxidase activity on the yield of grafted and ungrafted cucumber plants. Afric. J. of Agric. Res., 8(3), 279–283.

Kumar, G.N.M. (2011). Propagation of plants by grafting and budding (p. 16). Revised Edition. Washington: Horticultural and Landscape Architectur, Washington State University.

Mahunu, G.K., Adjei, P.Y., & Asante, A.K. (2012). Anatomical studies on graft formation in cashew (Anacardium occidentale L.). Agric. and Biol. J. of North Amer., 3(4), 150–153.

Mahunu, G.K., Osei-Kwarteng, M., & Quainoo, A.K. (2013). Dynamics of graft formation in fruit tress: A review. Albanian J. Agric. Sci., 12(2), 177–180.

Mng’omba, S.A., du Toit, E.S., & Akinnifesi, F.K. (2008). The relationship between graft incompatibility and phenol in Uapaca kirkirana Müell Arg. Scientia Horticulturae, 117, 212–218.

Pereira, I.D.S., Messias, R.D.S., Campos, A.D., Errera, P., Antunes. L.E.C., Fachinello, J.C., & Pina, A. (2014). Growth characteristics and phenylalanine ammonia-lyase activity in peach grafted on different Prunus spp. Biologia Plantarum, 58(1), 114–120.

Pina, A., & Errera, P. (2005). A review of new advances in mechanism of graft compatibility-incompatibility. Scientia Horticulturae, 106, 1–11.

Regazzoni, O., Sugito, Y., Suryanto, A., & Prawoto, A.A. (2015). Efisiensi penggunaan energi matahari klon-klon tanaman kakao (Theobroma cacao L.) yang diusahakan di bawah tiga spesies tanaman penaung. Pelita Perkebunan, 31(1), 21–29.

Stino, R.G., Ghoneim, E.I., Marwad, I.D., & Fadl, T.R. (2011). Performance of summer grafted superior seedless grapes grafts on different rootstock. J. of Hort. Sci. and Ornament. Plants, 3(1), 86–90.

Susilo, W.A., Anita-Sari, I., Sobandi, Suwitra, & Nurlia. (2012). Stabilitas daya hasil klon-klon harapan kakao (Theobroma cacao L.) tahan hama penggerek buah kakao. Pelita Perkebunan, 28(3), 123–135.

Susilo, A.W. (2013). Peran petani dalam pengembangan klon-klon lokal Sulawesi. Warta Pusat Penelitian Kopi dan Kakao, 25, 1–6.

Susilo, A.W., Anita-Sari, I., & Imran (2015). Yield performance of locally selected cocoa clones in North Luwu. Pelita Perkebunan, 31(3), 152–162.

Towaha, J., & Wardiana, E. (2015). Evaluasi tingkat toleransi 35 genotipe kakao terhadap periode kering. Jurnal Tanaman Industri dan Penyegar, 2(3), 133–142.

Zarrouk, O., Testillano, P.S., Risueňo, M.C., Moreno, M.A., & Gogorcena, Y. (2010). Changes in cell/tissue organization and peroxidase activity as markers for early detection of graft incompatibility in Peach/Plum combinations. J. Amer. Soc. Hort. Sci., 135(1), 9–17.



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