Mikropropagasi Pada Tanaman Stevia rebaudiana (Bertoni)

Parnidi Parnidi, Aprilia Ridhawati

Abstract


Stevia merupakan salah satu tanaman penghasil pemanis alami. Mikropropagasi stevia melalui kultur jaringan dapat menyediakan bahan tanaman secara massal dan cepat yang diperlukan untuk pengembangan stevia. Pada mikropopagasi melalui kultur jaringan diperlukan komposisi media yang tepat. Penelitian ini bertujuan untuk menguji komposisi media yang sesuai untuk mikropropagasi  tanaman stevia. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Pemanis dan Serat pada Februari - Juni 2016. Penelitian dilakukan menggunakan Rancangan Acak Lengkap (RAL) setiap perlakuan diulang tiga kali. Induksi tunas stevia menggunakan media dasar Murashige and Skoog (MS) dengan penambahan Benzil Amino Purin (BAP) dengan konsentrasi 0; 0,25; 0,5; 0,75 dan 1 mg/L. Induksi perakaran stevia menggunakan media dasar MS dengan dengan penambahan 1; 1,5; 2 dan 2,5 mg/L IAA, IBA dan NAA dan media MS tanpa penambahan ZPT sebagai kontrol. Hasil penelitian menunjukkan bahwa media MS + BAP 0,5 mg/L menunjukkan pertumbuhan terbaik dengan rerata jumlah tunas 17,80 dan rerata panjang tunas 3,25 cm. Media perakaran terbaik terdapat pada perlakuan media MS + IAA 1mg/L yang menghasilkan jumlah akar dengan rerata 4,60 dan panjang akar 2,27 cm.

ABSTRACT

Micropropagation of Stevia rebaudiana (Bertoni)

Stevia is one of the plants that produces natural sweeteners. Stevia micropropagation through tissue culture can provide a large of amount and fast plant material needed for stevia plantation. Micropropagation through tissue culture requires a proper media composition. This study aims to examine the composition of media suitable for stevia micropropagation. The study was conducted at the Tissue Culture Laboratory, Indonesian Sweeteners and Fiber Crops Research Institute in February - June 2016; using a completely randomized design (CRD) with three replicates. The treatment for shoot induction using Murashige and Skoog (MS) basic medium plus Benzyl Amino Purin (BAP) with concentrations: 0; 0.25; 0.5; 0.75 and 1 mg/L.  The treatment for root induction using MS basic medium with the addition of 1; 1,5; 2 and 2,5 mg/L IAA, IBA and NAA and for control using MS basic medium without the addition of plant growth regulators. The results showed that the best growth of stevia shoots with mean of shoots number 17.80 and mean of shoots length 3.25 cm was found in MS basic medium + BAP 0.5 mg/L. The best stevia root growth with mean of root number 4.60 and mean of root length 2.27 cm was found in MS basic medium + IAA 1 mg/L.


Keywords


stevia, induksi tunas, induksi perakaran; shoots induction; roots induction; in vitro

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References


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DOI: http://dx.doi.org/10.21082/btsm.v12n1.2020.45-53

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