PROSPEK PENGEMBANGAN BIOINSEKTISIDA NUCLEOPOLYHEDROVIRUS (NPV) UNTUK PENGENDALIAN / Prospect of Development of Nucleopolyhedrovirus (NPV) Bioinsecticide Against

Samsudin Samsudin

Abstract


Chemical insecticides for estate crop pests control are ineffective, impractical, expensive, and causing environmental pollutions. The entomopathogenic virus (EPV) mainly the nucleopolyhedrovirus (NPV) can be developed as an effective, efficient, and environmentally friendly biopesticide. NPV can be survived in the field in the form of polyhedra and spread naturally through the vertical and horizontal transmission process. The infected larvae usually hang by pseudolegs to the leaves or entrees. Research to increase virulence, host spectrum and its persistence has been done to overcome some weaknesses of NPV if developed as biopesticide. Some NPV isolates that infect the estate crop pests and potential to be developed in Indonesia, among others: Spodoptera litura NPV (SlNPV), S. exigua NPV (SeNPV), Helicoverpa armigera NPV (HaNPV), Sethotosea asigna NPV (SaNPV), Hyposidra talaca NPV (HtNPV) and Maenas maculifascia NPV (MmNPV). Based on several advantages of NPV compared with the chemical insecticides, the development of NPV biopesticide for controlling estate crop pests in Indonesia has very good prospects.


Keywords


Nucleopolyhedrovirus;bioinsecticide;pests;estate crops

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References


Adams, J.R. and J.R. Bonami. 1991. Atlas of Invertebrata Viruses. CRC Press: Boca Raton, Florida. 684 p.

Adams, J.R. and T.J. McClintock. 1991. Baculoviridae. Nuclear polyhedrosis virus. Part I. Nuclear polyhedrosis viruses of insects. In Adam, J.R. and J.R. Bonami. (Eds.) Atlas of Invertebrate Viruses. CRC Press: Boca Raton, Florida. 87-204.

Arifin, M. 1988. Pengaruh konsentrasi dan volume nuclear polyhedrosis virus terhadap kematian ulat grayak kedelai (Spodoptera litura F.). Penelitian Pertanian. 8(1): 12-14.

Arifin, M. 2006. Kompatibilitas SlNPV dengan HaNPV dalam Pengendalian Ulat Grayak dan Ulat Pemakan Polong Kedelai. Penelitian Pertanian Tanaman Pangan. 25 (1): 65-70.

Arifin, M. dan Bedjo. 2007. Keefektifan beberapa isolat SlNPV dan kombinasinya dalam pengendalian ulat grayak pada kedelai. Prosiding Seminar Nasional Komunikasi Hasil-hasil Penelitian Pertanian dan Peternakan dalam Sistem Usahatani Lahan Kering. Kupang, 7-8 Desember 2007. Buku 1: 222-228.

Arifin, M. 2010. Bioinsektisida SlNPV untuk mengendalikan ulat grayak mendukung swasembada kedelai. Orasi Pengukuhan Profesor Riset Bidang Entomologi (Hama dan Penyakit Tanaman) pada Badan Penelitian dan Pengembangan Pertanian, Kementerian Pertanian. Bogor, 6 September 2010. 54 p.

Armenta, R. dan A.M. Mertinez, J.W. Chapman, R. Magallanes, D. Goulson, P. Caballero, R.D. Cave, J. Cisneros, J. Valle, V. Castillejos, D.I. Penagos, L.F. Garcia, and T. William. 2003. Impact of a nucleopolyhedrovirus bioinsecticide and selected synthetic insecticides on the abundance of insect natural enemies on maize in Southern Mexico. J. Econ. Entomol. 96(3): 649-661.

Asano, S. 2005. Ultraviolet protection of a granulovirus product using iron oxide. Appl Entomol Zool, 40(2): 359-364.

Asri, M.T., Isnawati, dan M.T. Hidayat. 2003. Konsentrasi virus HaNPV isolat Yogyakarta yang efektif untuk mengendalikan ulat Helicoverpa armigera. Jurnal Pendidikan Matematika dan Sains, 3(8): 150-154.

Atmadja, W.R., T.E. Wahyono, dan N. Tarigan. 2009. Efektivitas patogen serangga sebagai agensia hayati untuk mengendalikan Maenas maculifascia pada tanaman ylang-ylang (Canangium odoratum). Bull. Littro, 2(1): 68-76.

Azis, A. 2009. Kajian potensi virus β-nudaurelia untuk pengendalian hama ulat api (Thosea asigna) pada tanaman kelapa. Jurnal Penelitian Pertanian Terapan, 9(2): 90-94.

Beard, C.B., J.F. Butler, and J.E. Muriniak. 1989. A Baculovirus in the flea, Pulex simulans. J. Invert. Pathol., 54:128-131.

Beas-Catena, A., A. Sanchez-Miron, F. Garcia- Camaacho, A. Contreras-Gomez, and E. Molina-Grima. 2014. Baculovirus biopesticides: an overview. The Journal of Animal & Plant Sciences, 24(2): 362-373.

Bedford, G.O. 1981. Control of Rhinoceros beetle by baculovirus In HD Burges (Eds.) Microbial Control of Pest and Plant Diseases 1970-1980. Academic Press, New York. 409-426.

Bedjo. 2011. Pemanfaatan biopestisida SlNPV dan HaNPV untuk pengendalian Spodoptera litura dan Helicoverpa armigera pada tanaman kedelai. Suara Perlindungan Tanaman, 1(3): 7-12.

Bedjo. 2012. Evaluasi beberapa isolat Helicoverpa amigera nuclear polyhedrosis virus (HaNPV) untuk pengendalian hama pemakan polong kacang hijau. Suara Perlindungan Tanaman, 2(1): 1-5.

Bonning, B.C. and B.D. Hammock. 1996. Development of recombinant baculoviruses for insect control. Annu. Rev. Entomol., 41: 191-210.

Bull, D.L., V.S. House, J.R. Ables, and R.K. Morrison. 1979. Selective methods for managing insect pests of cotton. J. Econ. Entomol., 72:841-846.

CAB INTERNATIONAL. 2000. Crop Protection Compendium. Wallingford, UK.

Castillejos, V., J. Trujillo, L.D. Ortega, J.A. Santizo, J. Cisneros, D.I. Penagos, J. Valle, and T. Williams. 2002. Granular phagostimulant nucleopolyhedro-virus formulations for control of Spodoptera frugiperda in maize. Biol. Contr., 24: 300- 310.

Chandler, D., A. Bailey, G.M. Tatchell, G. Davidson, J. Greaves, and W.P. Grant. 2011. The Development, Regulation and Use of Biopesticides for Integrated Pest Management. Philosophical Transaction of The Royal Society B, 386: 2-13.

Cunningham, J.C. and P.F. Entwistle. 1981. Control of Sawfly by Baculovirus, In Burges, H.D. (Eds.) Microbial Control of Pest and Plant Diseases 1970 - 1980. Academic Press, New York. P. 379-407.

Dodin, K., M. Arifin, dan Harnoto. 2001. Kompatibiltas SlNPV dengan ekstrak biji mimba untuk mengendalikan ulat grayak pada kedelai. Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman. 343-347.

Dougherty, E.M., N. Narang, M. Loeb, D.E. Lynn, and M. Shapiro. 2006. Fluorescent brightener inhibits apoptosis in baculovirus-infected gypsy moth larval midgut cells. Biocon. Sci. Tech. 16(1/2): 157-168.

Duraimurugan, P. and A. Regupathy. 2005. Push- pull strategy with trap crops, neem and nuclear polyhedrosis virus for insecticide resistance management in Helicoverpa armigera (Hubner) in cotton. American Journal of Applied Sciences. 2(6): 1042- 1048.

Dushoff, J. and G. Dwyer. 2001. Evaluating the risks of engineered viruses: modeling pathogen competition. Ecol. Appl. 11(6): 1602 - 1609.

Elvira, S., N. Gorria, D. Munoz, T. Williams, and P. Caballero. 2010. A simplified low-cost diet for rearing Spodoptera exigua (Lepidoptera: Noctuidae) and its effect on S. exigua nucleopolyhedrovirus production. J. Econ. Entomol. 103(1): 17- 24.

El Salamouny, S., D. Ranwala, M. Shapiro, B.M. Shepard, and B.R. Farrar. 2009. Tea, coffee, and cocoa as ultraviolet radiation protectants for the beet armyworm nucleopolyhedrovirus. J. Econ. Entomol., 102(5):1767-1773.

Erayya, J. Jagdish, P.K. Sajeesh, and V. Upadhyay. 2013. Nuclear Polyhedrosis Virus (NPV), A Potential Biopesticide: A Review. Research Journal of Agriculture and Forestry Sciences, 1(8): 30-33.

Etebari, K., L. Matindoost, S.Z. Mirhoseini, and M.W. Turnbull. 2007. The effect of BmNPV infection on protein metabolism in silkworm (Bombyx mori) larva. ISJ, 4: 13-17.

Farrar Jr.R.R., M. Shapiro, and I. Javaid. 2004. Photostabilized titanium dioxide and a fluorescent brightener as adjuvants for a nucleopolyhedrovirus. BioControl 48(4): 543-560.

Gasic, S. and B. Tanovic. 2013. Biopesticide Formulations, Possibility of Application and Future Trends. Pestic. Phytomed. Belgrade, 28(2): 97-102.

Glare, T.R., P.J. Walsh, M. Kay, and N.D. Barlow. 2003. Strategies for the eradication or control of gypsy moth in New Zealand. Agresearch. 165 p.

Gothama, A.A.A., G.W. Lawrence, and P.P. Sikorowski. 1996. Activity and persistence of Steinernema carpocapsae and Spodoptera exigua nuclear polyhedrosis virus against S. exigua larvae on soybean. J. Nematol. 28(1): 68-74.

Granados, R.R. and B.K. William. 1986. In Vivo Infection and Replication of Baculviruses in The Biology of Baculoviruses. CRC Press. Boca Raton, Florida. 90-104.

Gupta, R.K., M. Gani, P. Jasrotia, K. Srivastava, and V. Kaul. 2014. A comparison af infectivity between polyhedra of the Spodoptera litura multiple nucleopolyhedro- virus before and after passage through the gut of the stink bug Eocanthecona furcellata. Journal of Insect Science 14(96).

Hamm, J.J., L.D. Chandler, and H.R. Sumner. 1994. Field tests with fluorescent brightener to enhance infectivity of fall armyworm (Lepidoptera: Noctuidae) nuclear polyhedrosis virus. Florida Entomologist 77(4): 425-434.

Hammock, B.D., B.C. Bonning, R.D. Possee, T.N. Hanzlik, and S. Maeda. 1990. Expression and effects of juvenile hormone esterase in a baculovirus vector. Nature 344: 458 - 461.

Hoffmann, M.P. and A.C. Frodsham. 1993. Natural Enemies of Vegetable Insect Pest. Cooperative Extention. Cornell University. Ithaca. New York. 63 p.

Huger, A.M. 1966. A virus disease of the Indian rhinoceros beetle, Oryctes rhinoceros (L.) caused by a new type of insect virus, Rhabdionvirus oryctes gen. J. Invert. Pathol. 8: 38-51.

Ignoffo, C.M. and T.L. Cough. 1981. The Nucleopolyhedrosis virus of Heliothis spp. as a microbial insecticide In Burges HP (Ed.) Microbial Control of Pest dan Plant Diseases 1970-1980. Academic Press London dan New York, NY. 29-362.

Ignoffo, C.M., B.S. Shasha, and Shapiro. 1991. Sunlight ultraviolet protection of Heliothis nuclear polyhedrosis virus through starch-encapsulation technology. J. Invert. Pathol. 57: 134-136.

Inceoglu, A.B., S.G. Kamita, and B.D. Hammock. 2006. Genetically modified baculoviruses: a historical overview and future outlook. Adven. Vir. Res. 68: 323- 326.

Ishikawa, H., M. Ikeda, C.A.F. Alves, S.M. Theim, and M. Kobayashi. 2004. Host range factor 1 from Lymantria dispar nucleopolyhedrovirus (NPV) is an essential viral factor required for productive infection of NPVs in IPLB- Ld652Y cells derived from L. dispar. J. Virol. 78(22):12703 - 12708.

Jeyarani, S. N. Sathiah, and P. Karuppuchamy. 2005. Influence of Pseudomonas fluorescens and a Nucleopolyhedrovirus on Cotton Bollworm Helicoverpa armigera (Hubner). Tropical Agricultural Research, 17: 221- 230.

Kalmakoff and Ward. 2003. Baculoviruses. Univerdity of Otago, Dunedin, New Zealand. http://www.microbiology- bytes.com/virology. [Desember 2011].

Kao, S.S., W.T. Hsia, and L.H. Huang. 1991. Effectiveness of adjuvants for nuclear polyhedrosis virus against the beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae). Chinese J. Entomol. 11: 330-334.

Kumar, N. S., K. Murugan, and W. Zhang. 2007. Additive interaction of Helicoverpa armigera Nucleopolyhedrovirus and Azadirachtin. Biocontrol, 53: 869-880.

Lacey, L.A., R. Frutos, H.K. Kaya, and P. Vail. 2001. Insect pathogens as biological control agents: do they have a future?. Biol. Cont. 21: 230-248.

Lasa, R., C. Ruiz-Portero, M.D. Alcazar, J.E. Belda, P. Caballero, and T. William. 2007. Efficacy of optical brightener formulations of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) as a biological in greenhouse of Southern Spain. Biol. Cont. 40: 89-96.

Lasa, R, T. William, and P. Caballero. 2008. Insecticidal properties and microbial contaminants in Spodoptera exigua multiple nucleopolyhedrovirus (Baculoviridae) formulation stored at different temperatures. J. Econ. Entomol. 101(1):42-49.

Li, Z. and G.W. Blissard. 2009. The Autographa californica multicapsid nucleopolyhedrovirus GP64 protein: Analysis of transmembrane domain length and sequence requirements. J. Virol. 89(9):4447-4461.

Maeda, S. 1989. Increased insecticidal effect of a recombinant baculovirus carrying a synthetic diuretic hormone. Biochem. Biophys. Res. Comm. 165: 1177 - 1183.

Martinez, A.M., O. Simon, T. Williams, and P. Caballero. 2003. Effect of optical brighteners on the insecticidal activity of a nucleopolyhedrovirus in three instars of Spodoptera frugiperda. Ent. Exp. App. 109: 139-146.

McCutchen, B.F., P.V. Choudary, R. Crenshaw, D.W. Maddox, K. Majima, B.D. Hammock, and E. Fowler. 1991. Insecticidal effects of an insect-specific neurotoxin expressed by recombinant baculovirus. Virol. 184: 777 - 780.

McIntosh, A.H., J.J. Grasela, L. Lua, and S.C. Braunagel. 2004. Demonstration of the effects of fluorescent proteins in baculoviruses exposed to ultraviolet light inactivation. J. Insect. Sci. 4:31-39.

Mehrvar, A., R.J. Rabindra, K. Veenakumari, and G.B. Narabenchi. 2008. Evaluation of adjuvants for increased of HearNPV against Helicoverpa armigera (Hubner) using suntest machine. J. Biol. Sci. 1-8.

Mondragon, G., S. Pineda, A. Martinez, and A.M. Martinez. 2007. Optical brightener Tinopal C1101 as an ultraviolet protectant for a nucleopolyhedro-virus. Commun. Agric. Appl. Biol. Sci. 72(3): 543-547.

Monobrullah, Md. 2003. Optical brighteners- pathogenicity enhancers of entomo- pathogenic viruses. Current Sci., 84(5): 640 - 645.

Murillo, R., R. Lasa, D. Goulson, T. Williams, D. Munoz, and P. Caballero. 2003. Effect of tinopal LPW on the insecticidal properties and genetic stability of the nucleopolyhedrovirus of Spodoptera exigua (Lepidoptera: Noctuidae). J. Econ. Entomol., 96(6): 841-846.

Narayanan, K. 2004. Insect defence: its impact on microbial control of insect pests. Current Sci. 86(6): 800-814.

Okada, M. 1977. Studies on the utilization and mass production of SlNPV for control of the tobacco cutworm, Spodoptera litura F. Rev. Pl. Protec. Res. 10: 102-128.

O’Reilly, D.R. and L.K. Miller. 1991. Improvement of a baculovirus pesticide by deletion of the egt gene. Bio/Tech. 9: 1086-1089.

Pionar, O.G. Jr. and G.M. Thomas. 1984. Laboratory Guide to Insect Pathogens and Parasites. Plenum Press, New York. 392 p.

Ramle, M., M.B. Wahid, K. Norman, T.R. Glare, and T.A. Jackson. 2005. The incidence and use of Oryctes virus for control of rhinoceros beetle in oil palm Plantation in Malaysia. Journal of Invertebrate Pathology. 89: 85-90.

Samsudin. 1999. Karakterisasi virus patogen dari ulat bawang Spodoptera exigua (Lepidoptera: Noctuidae) isolat indonesia. Tesis Program Magister Sekolah Pascasarjana Institut Pertanian Bogor. 56 h.

Samsudin. 2001. Keefektifan kombinasi Anagrapha falcifera NPV (AfNPV) dengan Spodoptera litura NPV (SlNPV) untuk mengendalikan ulat grayak pada kedelai. Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman, Bogor, 26-27 Desember 2001. 348-351.

Samsudin. 2011. Uji patologi dan perbaikan kinerja Spodoptera exigua nucleopolyhedrovirus (SeNPV). Disertasi Program Doktor Sekolah Pascasarjana Institut Pertanian Bogor. 109 h.

Samsudin, T. Santoso, A. Rauf, dan Y.M. Kusumah. 2011. Keefektifan bahan pelindung alami dalam mempertahankan infektifitas Spodoptera exigua nucleopolyhedrovirus (SeNPV). Berita Biologi. 10(6): 689-697.

Samsudin. 2012. Virus patogen serangga sebagai agens hayati pengendali hama tanaman perkebunan yang efektif. Info Tek. 4(4): 15.

Samsudin dan T. Santoso. 2012. Infektifitas Spodoptera exigua nucleopolyhedrovirus (SeNPV) yang diperkaya dengan bahan pengaktif terhadap larva Spodoptera exigua Huebner. Berita Biologi. 11(3): 297- 304.

Samsudin dan T. Santoso. 2014. Uji Patologi Spodoptera exigua Nucleopolyhedrovirus (SeNPV) pada Larva S. exigua Huebner (Lepidoptera: Noctuidae). Jurnal Biologi Indonesia. 10(2): 169-178.

Shapiro, M., and B.M. Shepard. 2008. Relative efficacies of congo red and tinopal LPW on the activity of the gypsy moth (Lepidoptera: Lymantriidae) nucleopoly- hedrovirus and cypovirus. J. Agric. Urban. Entomol. 25(4): 233-243.

Shapiro, M., S.E. Salamouny, and B.M. Shepard. 2008. Green tea extracts as ultraviolet protectants for the beet armyworm, Spodoptera exigua, nucleopolyhedrovirus. Biocon Sci. Tech. 18: 591-603.

Shepard, E.F., B.M. Shepard, and A. Rauf. 1996. Virus of Spodoptera exigua. Palawija/ Vegetable IPM Newsletter. 1(1) : 2-3.

Spenger, A.R., Grabherr, L. Tollner, H. Katinger, and W. Ernst. 2002. Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of envelope protein gp64. Eur. J. Biochem. 269: 4458 - 4467.

Stairs, G.R. and T. Fraser. 1981. Changes in growth dan virulence of nuclear polyhedrosis virus. J. Inver. Pathol. 35:230-235.

Sudharto, A. Susanto, R.Y. Purba, dan B. Drajat. 2011. Teknologi Pengendalian Hama dan Penyakit pada Kelapa Sawit: Siap Pakai dan Ramah Lingkungan. Pusat Penelitian Kelapa Sawit. 3 h.

Suhas, Y., J.B. Gopali, B.V. Patil, and S. Lingappa. 2009. Effect of different adjuvants in enhancing the efficacy of HaNPV against Helicoperva armigera (Hubner) in pigeonpea. Karnataka J. Agric. Sci. 22(3): 502-503.

Takatsuka, J. and Y. Kunimi. 2002. Lethal effects of Spodoptera exigua nucleopoly- hedrovirus isolated in Shiga Prefecture, Japan, on larvae of the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae). Appl. Entomol. Zool. 37(1): 93-101.

Tanada, Y. and R.T. Hess. 1991. Baculoviridae. Granulosis viruses. In Adam, J.R. and J.R. Bonami (Eds.). Atlas of Invertebrate Viruses. CRC Press: Boca Raton, Florida. 227 - 257.

Tanada, Y. and H.K. Kaya. 1993. Insect Pathology. Academic Press. San Diego. California. p. 78-98.

Tinsley, T.W. and D.C. Kelly. 1985. Taxonomy and Nomenclatures of Insect Pathogenic Viruses. p. 3-26. In Maramorosch, K. and Sherman, K.E. (Eds.). Viral Insectisides for Biological Control. Academic Press. London.

Treacy, M.F. 1999. Recombinant baculoviruses. In Hall, F.R. and J.M. Julius. 1999. Biopesticides Use and Delivery. Humana Press. Totowa, New Jersey. 321 - 340.

Vilaplana, L., K. Wilson, E.M. Redman, and J.S. Cory. 2010. Pathogen persitence in migratory insects: high levels of vertically-transmitted virus infection in field populations of the African armyworm. Evol. Ecol. 24: 147-160.

Washburn, J.O., D. Trudeau, J.F. Wong, and L.E. Volkman. 2003. Early pathogenesis of Autographa californica multiple nucleopolyhedrovirus and Helicoverpa zea single nucleopolyhedrovirus in Heliothis virescensDZ a comparison of the ‘M’ and ‘S’ strategies for establishing fatal infection. J. Gen Virol., 84: 343-351.

Wijarnako, A. 1998. Mengendalikan hama dengan musuh alami. Institute For Science and Technology Studies Chapter Japan. htt://istecs.org/Publication/ Dimensi].

Woo, S.D., J.Y. Roh, J.Y. Choi, and B.R. Jin. 2007. Propagation of Bombyx mori nucleopolyhedrovirus in nonpermissive insect cell lines. J. Microbiol., 45(2): 133- 138.

Young, S.Y. 1989. Problems associated with the production and use of viral pesticides. Mem. Inst. Oswaldo Cruz. 84(3): 67-73.




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