PEMANFAATAN BAKTERI ENDOFIT DAN FOSFAT UNTUK PERTUMBUHAN DAN PRODUKSI TANAMAN SAMBILOTO / Utilizing Endophytic Bacteria and Phosphate for Growth and Yieald of Andrographis paniculata

Gusmaini Gusmaini, Didy Sopandie, Sandra Arifin Aziz, Abdul Munif, Nurliani Bermawie

Abstract


Andrographis paniculta produce andrographolide, which have functions as raw material for medicine. One can stimulate andrographolide yield by utilizing endophytic bacteria and phosphate. This research objectives were to obtain effect of endophytic bacteria and phosphate on growth, yield and andrographolide content, N, P, and K uptake of A. paniculta. The research was conducted at the experimental garden of ISMCRI, at Cimanggu, Bogor from June 2012-February 2013. The trial was arranged in Random Block Design, factorial, 9 treatments and 3 replications. The first factor were endophytic bacteria; 1) without endophytic bacteria, 2) 20CD, and 3) 20BB. The second factor were the levels of P; 1) without P, 2) 27 kg/ha P, and 3) 54 kg/ha P. The results showed that Endophytic bacteria application and P fertilizer significantly increased plant growth, dry matter yield, andrographolide content and yield, and N, P, K uptake, but there were no interaction between endophytic bacteria and P treatments. Giving of endophytic bacteria improved content and yield of andrographolide ranging 16,9-29,9% and 37,6-45,7% respectively. A dossage of 27 kg/ha P was recommended to produce dry matter and andrographolide yield, and andrographolide content obtained 2.56%. The best andrographolide yield should be harvested at the beginning of generative phase.

Keywords: Andrographis paniculata, andrographolide content, endophytic bacteria, phosphate, yield.

 

Abstrak

Tanaman sambiloto merupakan tanaman penghasil andrografolid, yang berfungsi sebagai bahan baku obat. Salah satu yang dapat memacu produksi andrographolid tersebut dengan memanfaatkan bakteri endofit dan fosfat. Penelitian bertujuan untuk mengetahui pengaruh bakteri endofit dan fosfat terhadap pertumbuhan, produksi dan kadar andrografolid serta serapan hara N, P, dan K pada tanaman sambiloto. Penelitian dilaksanakan di Kebun Percobaan Cimanggu Balai Penelitian Tanaman Rempah dan Obat (Balittro), Bogor dari bulan Juni 2012-Februari 2013. Penelitian ini menggunakan rancangan acak kelompok, faktorial, 9 perlakuan dan 3 ulangan. Faktor pertama yaitu bakteri endofit; 1) tanpa bakteri, 2) 20CD, dan 3) 20BB. Faktor kedua adalah dosis pupuk P; 1) tanpa pupuk, 2) 27 kg/ha P, dan 3) 54 kg/ha P. Hasil penelitian menunjukkan bahwa bakteri endofit dan P nyata meningkatkan pertumbuhan, produksi biomas, kadar dan produksi andrografolid, serta serapan hara N, P, dan K pada umur tanaman 14 MST, namun tidak terdapat interaksi antara kedua faktor tersebut. Pemberian bakteri endofit dapat meningkatkan kadar dan produksi andrografolid, masing-masing berkisar 16,9-29,9% dan 37,6- 45,7%. Dosis 27 kg/ha P direkomendasikan untuk menghasilkan produksi bahan kering dan produksi andrografolid, dengan kadar andrografolid yang diperoleh 2,56%. Produksi andrografolid terbaik, tanaman dipanen pada fase awal generatif.

Kata kunci: Andrographis paniculata, andrografolid, bakteri endofit, pertumbuhan, fosfat, produksi.


Keywords


Andrographis paniculata;andrographolide content;endophytic bacteria;phosphate;yield

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References


ANURADHA, V.E., C.A.JALEEL, M.A. SALEM, M. GOMATHINAYAGAM , and R. PANNEERSELVAM . 2010. Plant growth regulators induced changes in antioxidant potential and andrographolide content in Andrographis paniculata Wall.ex Nees. Pest. Biochem. and Physi. 98: 312-316.

ARPANA, J., and D.J. BAGYARAJ. 2007. Response of kalmegh to an arbuscular mycorrizhal fungus and a plant growth promoting rhizomicroorganism at two levels of phosphorus fertilizer. Am-Euras J. Agric. and Environ. Sci. 2(1): 33-38.

ASIS, C.A.J.R., K. ADACHI and S. AKAO. 2004. N2 fixation in sugarcane and population of N2 fixing endophytes in stem apoplast solution. Philip. J. Crop. Sci. 29 (2): 45- 58.

BERTHAM, R.Y.H., dan A.D. NUSANTARA. 2011. Mekanisme adaptasi genotipe baru kedelai dalam mendapatkan hara fosfor dari tanah mineral masam. J. Agron. Indones. 39(1): 24-30.

BOIERO et al. Phytohormone production by three strains of Bradyrhizobium japonicum and possible physiological and technological implications. Appl. Microbiol. Biotech. 74: 874-880.

BRIELMANN, H.L., W.N. SETZER, P.B. KAUFMAN, A . KIRAKOSYAN, and L.J. CSEKE. 2 006. Phytochemicals: the chemical components of plants in natural products from plants. 2nd Edition,’ T aylor & Francis. Boca Raton, London, New York.

CHENG, A.X., Y.G. LOU, Y. BO MAO, S. LU, L. JIAN WANG, and X. YA CHEN. 2007. Plant terpenoids: biosynthesis and ecological functions. Invited Review. J. Integ. Plant Biol. 49 (2): 179-186.

COMPANT, S., C. CLEMENT, and A. SESSITSCH. 2005. Plant growth-promoting bacteria in the rhizo- and endosphere of plants: their role, colonization, mechanisms involved and prospects for utilization. Soil Biol. and Biochem. 42: 669-678.

FENG, Y., D. SHEN, and W. SONG. 2006. Rice endophyte Pantoea agglomerans YS19 promotes host plant growth and affects allocations of host photosynthate. J. of Appl. Microb. 100: 938-945.

GUDHATE, P.P., D.P. LOKHANDE, and K.N DHUMA. 20 09. Role of plant growth regulators for improving andrographolide in Andrographis paniculata . Res. Article. 5(19): 249-253.

GUO, B., Y. WANG, X. SUN, and K. TANG. 2008. Bioactive natural products from endophytes: A Review. Appl. Biochem. and Microbiol. 44(2): 136-142.

GUSMAINI, D. SOPANDIE, S.A. AZIZ, A. MUNIF, dan N. BERMAWIE. 2013. Potensi bakteri endofit dalam meningkatkan pertumbuhan, produksi dan kadar andrografolid pada tanaman sambiloto. Jurnal Littri, 19(4): 167-177.

HARTOYO, B. 2012. Efektivitas fungi mikoriza arbuskula pada penggunaan pupuk fosfor alami dan pengaruhnya terhadap pertumbuhan, biomassa dan produksi asiatikosida pegagan (Centella asiatica L. Urban) di andosol. [Disertasi]. Sekolah Pasca Sarjana IPB, Bogor.

HENDAWAY, S. F., and K.A. KHALID. 2011. Effect of chemical and organic fertilizers on yield and essential oil chamomile flower head. Med. And Arom. Plant Sci. Biotech. 5(1): 43-49.

LUGTENBER B., and F. KAMILOVA. 2009. Plant-growth- promoting rhizobacteria. Ann. Rev.of Microb.. 63: 541-556.

MATSUOKA, H., M. AKIYAMA, K. KOBAYASHI, and K. YAMAJI. 2012. Fe and P solubilization under limiting condition by bacteria isolated from Carex kobomugi roots at the hasaki coast. Curr. Microb. 66(3): 314-321.

MILIŪTĖ, I., O. BUZAITĖ. 2011. IAA production and other plant growth promoting traits of endophytic bacteria from apple tree. Biologija. 57(2): 98-102.

NURAINI, L. 2011. Pengaruh abu volkan dari berbagai ketinggian tempat, pupuk fosfat, dan zeolit terhadap pertumbuhan dan kualitas hasil sambiloto (Andrographis paniculata Ness.)’, [Thesis], Sekolah Pasca Sarjana UGM, Yogyakarta.

PANHWAR , Q.A., O. RADZIAH, M. SARIAH, and M.R. ISMAIL. 2012. Solubilization of different phosphate forms by phosphate solubilizing bacteria isolated from aerobic rice. Int. J. Agric. Biol. 11(6): 667-673.

PRABHU, M., A.R. KUMAR, K. RAJAMANI. 2009. Influence of bio-stimulants on growth, yield and economics of kalmegh Andrographis paniculata. Madras Agric. J. 96(1-6): 150-155.

QUECINE, M.C., W.L. ARAÚJO, P.B. ROSSETTO, A. FERREIRA, S. TSUI, P.T. LACAVA, M. MONDIN, J.L. AZEVEDO, A.A.P. KLEINER. 2012. Sugarcane growth promotion by the endophytic bacterium Pantoea agglomerans 33.1. App. Environ. Microb. 78(21): 7511-7518.

RAO, Y.K., VIMALAMMA G, RAO CV, TZENG Y. 2004. Flavonoids and andrographolides from Andrographis paniculata. Phytochem. 65: 2317-2321.

REGHUVARAN, A., K. KALA, JACOB, and A.D. RAVINDRANATH. 2012. Isolation and characterization of nitrogen fixing bacteria from raw coir pith. African. J. Biotech. 11 (27): 7063-7071.

RODRIGUEZ, H., and R. FRAGA. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotech. Adv. 17: 319-339.

SABER, et al. 2005. Effect of P on nodule formation and N fixation in bean Agron. Sustain. Dev. 389-393.

SHI, Y., K. LOU, and C. LI. 2009. Promotion of plant growth by phytohormone-producing endophytic mirrobs of sugar bit. Biol. Fertil. Soils. 45: 645-653.

SRIVASTAVA , N., and A. AKHILA. 2010. Biosynthesis of andrographolide in Andrographis paniculata’, Phytochem. 71: 1298-1304.

SUTARDI. 2011. Kajian waktu panen dan pemupukan fosfor terhadap pertumbuhan dan produksi asiatikosida tanaman pegagan (Centella asiatica L. Urban) di dataran tinggi’, [Tesis], Sekolah Pasca Sarjana IPB, Bogor.

van LOON, L.C., and P.A.H.M. BAKKER. 2005. Induced systemic resistance as a mechanism of disease suppression by rhizobacteria. In: Siddiqui, Z.A. (Ed.), PGPR: Biocontrol and Biofertilization. Springer, Dordrecht, The Netherlands, pp. 39-66.

VANCE, C.P., C.U. STONE, and D.L. ALLAN. 2003. Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. Tansley Review, New Patologist. 157: 423-447.

VIDYALAKSHMI, A., and S. ANANTHI. 2013. Induction of andrographolide, a biologically active ingredient in callus of Andrographis paniculata (Burm.F) Wallich ex. Nees’, Bioengin. and Biosci. 1(1): 1-4.

WAHID, A., S. GELANI, M. ASHRAF, and M.R. FOOLAD. 2007. Heat tolerance in plants: an overview. Environmental and Experimental Botany. 61: 199 -223.

XU, Y., R.I. MARSHALL, and T.K.S. MUKKUR. 2006. An investigation on the antimicrobial activity of Andrographis paniculata extract and andrographolide in vitro. Asian J. Plant Sci. 5: 527-530.

YUSRON, M., M. JANUWATI , dan E.R. PRIBADI. 2005. Standar prosedur operasional budidaya sambiloto dalam Kencur, Temulawak, Kunyit, Sambiloto dan Pegagan. Balai Penelitian Tanaman Rempah dan Obat. Sirkuler, 11: 37-42.

ZHANG, Z., J. JIANG, P. YU, X. ZENG, J.W. LARRICK, and J. WANG. 2009. Hypoglycemic and beta cell protective effects of andrographolide analogue for diabetes treatment. J. Trans. Med. 7: 62-64.




DOI: http://dx.doi.org/10.21082/littri.v22n3.2016.153-159

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