SINERGISME ANTARA NEMATODA PATOGEN SERANGGA Steinernema sp. DAN MINYAK BIJI JARAK PAGAR (Jatropha curcas L.) TERHADAP MORTALITAS DAN EFEK LANJUTAN LARVA Spodoptera litura F.

. Tukimin, Elna Karmawati, Heri Prabowo

Abstract


ABSTRAK

Penggunaan   insektisida   kimia   untuk   pengendalian   serangga menimbulkan dampak negatif. Oleh karena itu, diperlukan pengendalian hama yang ramah lingkungan. Nematoda patogen serangga dan pestisida nabati   minyak   biji   jarak   pagar (MBJP)  memiliki  peluang  untuk dikembangkan sebagai agensia pengendali Spodoptera litura F. Tujuan penelitian  ini  untuk  mengevaluasi  efek  kombinasi  antara  nematoda patogen serangga Steinernema sp. dan pestisida nabati MBJP terhadap mortalitas dan efek lanjutan larva S. litura. Penelitian dilaksanakan di Laboratorium Entomologi, Balai Penelitian Tanaman Pemanis dan Serat pada  bulan  Maret  sampai  Juni 2012,  menggunakan  rancangan  acak kelompok (RAK) pola faktorial dengan 4 ulangan. Perlakuan konsentrasi MBJP yang digunakan adalah 2,5 ; 5 ; 10 dan 20 ml/l, kontrol air, serta kontrol air + 1 g deterjen. Konsentrasi Steinernema sp. adalah 0, 200, 400, dan 800 JI (juvenil infektif)/ml. Nematoda Steinernema sp. dimasukkan ke dalam larutan pada saat aplikasi larva S. litura sebanyak 25 larva instar dua tiap perlakuan. Jumlah larva yang mati diamati pada 24, 48, 72, 96, dan 120 jam setelah penyemprotan. Hasil penelitian menunjukkan bahwa kombinasi   konsentrasi   antara   MBJP   dan   Steinernema   sp.   yang menghasilkan mortalitas larva S. litura tertinggi adalah 10 ml/l dan 400 JI/l atau 2,5 ml/l dan 800 JI/l. Efek lanjut MBJP mengakibatkan kerusakan pada fase prapupa dan pupa, yaitu menjadi cacat serta penurunan jumlah telur dan kemampuan telur menetas. Kenaikan konsentrasi MBJP dari 2,5 ke 20 ml/l mengakibatkan penurunan kemampuan penetasan telur dari 76,29 menjadi 32,37%.

Kata kunci: Steinernema sp., Jatropha curcas, Spodoptera litura


Sinergism between Entomopathogenic Nematodes Steinernema sp. and Physic Nut Oil (Jatropha curcas L.) against Mortality and Continuous Effect ofSpodoptera litura F. Larvae  

 

ABSTRACT

The use of chemical insecticides to control pests causes various negative impacts. Thus, environmentally friendly methods of control are much in need recently. The combination of entomopathogenic nematodes and botanical insecticides based on physic nut oil (PNO) has opened up new possibilities of promising control methods against Spodoptera litura F. The objective of the study was to evaluate the combination effect between botanical insecticides PNO and the entomopathogenic nematode Steinernema sp. on S. litura. The research was conducted at the laboratory of Entomology Indonesian Sweetener and Fiber Crops Research Institute from March to June 2012. The treatments were arranged in a completely randomized block design (CRBD) with factorial pattern, replicated four times. The concentrations of PNO were 2,5; 5; 10, and 20 ml/l, control water and control water + 1 g detergent. Concentration of Steinernema sp. were 0,200, 400, and 800 IJ (infective juvenile)/ml. Nematodes were added simultaneously after application of PNO. Insect larvae were exposed to various concentrations of Steinernema sp. and PNO. Numbers of dead larvae were observed at 24, 48, 72, 96, 120 hours after spraying. The result showed that the concentration combination between PNO and nematode which resulted maximum larvae mortality is 10 ml/l and 400 IJ/ml or 2,5 ml/l and 800 IJ/ml. The impacts of PNO were disabled prepupa and pupa, decrease  of  eggs  number  and  hatching  ability.  The  increase  of concentration used from 2.5 to 20 ml/l resulted the decreased of hatching from 76.29 to 36.37%.

Key words: Steinernema sp., Jatropha curcas, Spodoptera litura



Keywords


Steinernema sp., Jatropha curcas, Spodoptera litura

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DOI: http://dx.doi.org/10.21082/littri.v20n2.2014.93%20-%20100

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