PENGUJIAN BAKTERI ENDOFIT TERHADAP EFISIENSI HARA PADA PERTUMBUHAN TANAMAN LADA DI LAMPUNG / The Evaluation of Endophytic Bacteria Application on Nutrient Efficiency of Black Pepper Growth in Lampung

Gusmaini Gusmaini, Andriana Kartikawati, Hera Nurhayati

Abstract


Black pepper (Piper nigrum L.) requires high nutrients to support its growth. The research was carried out to find an alternative way to reduce high nutrient requirements with the application of growth-promoting bacteria. The research was conducted at Natar Experimental Station, Lampung Assessment Institute for Agricultural Technology (AIAT), from January to December 2018. The study was undertaken in a randomized block design, arranged factorially, with 16 treatments and 3 replications. The first factor was endophytic bacteria application consisted of: without endophytic bacteria (B0), Ca2 endophytic bacterial isolates (B1), isolates combination A (Dj9 + Sa4 + Labt8 + Ldbp4) (B2), and isolates combination B (Sa8 + Sd10 + Labt1 + Ldbp9 (B3). The second factor was fertilizer application comprised of: without fertilizer (P0), 25% (P1), 50% (P2), and 75% (P3) of recommendation dosage (NPKMg: 12: 12: 17: 2). The recommended fertilizer dose was 200 kg/ha/year. The results showed that there was an interaction between endophytic bacteria and fertilizer in enhancing plant growth and NPK nutrient uptake of pepper at 11 months after planting. The combination of Ca2 endophytic bacteria and 75% NPK fertilizer recommendations enhanced plant growth by 19.5% for plant height, 34.3% for the number of nodes, and 16.8% for the number of branches, compared to that without endophytic bacteria application. However, the combination of B2 endophytic bacteria and 75% of NPK fertilizer recommendations showed the best result for biomass weight and nutrient uptake. Endophytic bacteria can improve the effectiveness of organic fertilizer usage to support sustainable agriculture.

Keywords: Piper nigrum, nutrition, improvement, PGPB.

 

Abstrak

Lada (Piper nigrum L.) merupakan tanaman yang memerlukan hara cukup tinggi untuk pertumbuhannya. Penelitian ini dilakukan sebagai upaya untuk mengurangi pemberian hara yang cukup tinggi yaitu dengan memberikan bakteri pemicu pertumbuhan. Penelitian telah dilakukan di Kebun Percobaan Natar BPTP Lampung, dimulai dari bulan Januari-Desember 2018. Penelitian menggunakan rancangan acak kelompok, faktorial, 16 perlakuan dan 3 ulangan. Perlakuan terdiri dari faktor pertama penggunaan bakteri endofit yaitu; tanpa bakteri endofit (B0), isolat bakteri endofit Ca2 (B1), isolat kombinasi A (Dj9+Sa4+Labt8+Ldbp4) (B2), dan isolat kombinasi B (Sa8+Sd10+Labt1+Ldbp9) (B3). Faktor kedua pemupukan anorganik yaitu; tanpa pupuk (P0), 25% (P1), 50% (P2), dan 75% (P3) dosis rekomendasi (NPKMg: 12:12:17:2). Dosis rekomendasi adalah 200 kg/ha/tahun. Hasil penelitian menunjukkan adanya interaksi antara bakteri endofit dan pupuk terhadap peningkatan pertumbuhan, dan serapan hara NPK pada tanaman lada berumur 11 bulan setelah tanam. Pemberian bakteri endofit dapat meningkatkan tinggi tanaman 19,5%, jumlah ruas 34,3%, dan jumlah cabang 16,8% pada kombinasi bakteri endofit B1 dibandingkan tanaman tanpa pemberian bakteri endofit dan 75% rekomendasi pupuk NPK. Namun kombinasi perlakuan terbaik untuk bobot biomas dan serapan hara adalah pada kombinasi perlakuan bakteri endofit B2 dan 75% rekomendasi pupuk NPK. Bakteri endofit dapat berfungsi sebagai pupuk hayati dan dapat mengefisienkan penggunaan pupuk buatan untuk pertanian berkelanjutan.

Kata kunci: Piper nigrum, nutrisi, peningkatan,PGPB.


Keywords


Piper nigrum; nutrition; improvement; PGPB.

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References


Baca, B.E. & Elmerich, C. (2007) Microbial Production of Plant Hormones.In: Elmerich, C. & Newton, W. (eds.) Associative and Endophytic Nitrogen-Fixing Bacteria and Cyanobacterial Associations. Springer, pp.113–143.

Banik, A., Mukhopadhaya, S.K. & Dangar, T.K. (2016) Characterization of N2-fixing plant growth promoting endophytic and epiphytic bacterial community of Indian cultivated and wild rice (Oryza Spp.) genotypes. Planta. [Online] 243 (3), Springer Berlin Heidelberg, 799–812. Available from: doi:10.1007/s00425-015-2444-8.

Ditjenbun (2017) Statistik Perkebunan Indonesia 2015-2017. Lada. Hendaryati, D.D. & Arianto, Y. (eds.) Jakarta, Direktorat Jenderal Perkebunan.

Fulchieri, M., Lucangeli, C. & Bottini, R. (1993) Inoculation with Azospirillum lipoferum Affects Growth and Gibberellin Status of Corn Seedling Roots. Plant and Cell Physiology. 34 (8), Oxford University Press, 1305–1309.

Gusmaini, Aziz, S.A., Munif, A., Sopandie, D. & Bermawie, N. (2013) Isolation and selection of endophytic bacteria consortia from medicinal plant (Andrographis paniculata) as plant growth promoting agents. Journal of Agronomy. 12 (3), 113–121.

Gusmaini & Kartikawati, A. (2015) Isolasi dan Karakterisasi Bakteri Endofit Berasal dari Tanaman Lada Bangka. In: Gusmaini, Bermawie, N., Rizal, M., Wahyuno, D., Pribadi, E.R. & Nurhayati, H. (eds.) Prosiding Seminar Teknologi Cengkeh, Lada dan Pala. Bogor, IAARD Press, pp.159–168.

Gusmaini, Sopandie, D., Azis, S.A., Abdul Munif, D. & Bermawie, N. (2016) Utilizing endophytic bacteria and phosphate for growth and yield of Andrographis paniculata. Jurnal Littri. 22 (3), 151–157.

Hardjowigeno, S. (2007) Ilmu Tanah. Jakarta, Akademika Pressindo.

Harjoso, T. & Purwantono, A.S.D. (2002) Pemanfaatan Tanah Podzolik Merah Kuning Melalui Pemberian Pupuk Kandang dan EM4 bagi Program Pengembangan Baby Corn. Pembangunan Pedesaan. 2 (2), 27–33.

Hayman, D.S. (1975) Occurrence of Mycorrhiza in Crops as Affected by Soil Fertility.In: Sanders, F.E., Mosse, B. & Tinker, P.B. (eds.) Endomycorrhizas. London, Academic Press, pp.495–509.

Hidayati, U., Chaniago, I.A. & Munif, A. (2014) The Potency of mixed culture of endophytic bacteria as plant growth promoting for rubber rootstock growth. Indonesian J. Nat. Rubb. Res. 32 (2), 129–138.

Jipelos, J.M. (1989) Uptake of Nitrogen from Urea Fertiliser for Rice and Oil Palm-the Malaysian Experience. Nutrient Management for Food Crop Production in Tropical Farming Systems. Institute for Soil Fertility, 187–203.

Kartikawati, A. & Gusmaini (2018) Potensi bakteri endofit yang diisolasi dari tanaman jahe merah untuk memacu pertumbuhan benih lada. Bul Littro. 29 (1), 37–46.

Liu, T.Z., Zhang, J.M., Mao, Z.W. & Li, R.J. (2016) Influence of endophytic diazotroph and nitrogen fertilization on the growth and turf quality of ‘TifEagle’ Bermudagrass. European Journal of Horticultural Science. 81 (4), 227–233.

Mardad, I., Serrano, A. & Soukri, A. (2013) Solubilization of inorganic phosphate and production of organic acids by bacteria isolated from a moroccan mineral phosphate deposit. African Journal of Microbiology Research. [Online] 7 (8), 626–635. Available from: doi:10.5897/AJMR12.1431.

Nath, R., Sharma, G.D. & Barooah, M. (2012) Efficiency of tricalcium phosphate solubilization by two different endophytic Penicillium sp . Isolated from Tea (Camellia sinensis L.). European Journal of Experimental Biology. 2 (4), 1354–1358.

Ngamau, C.N., Matiru, V.N., Tani, A. & Wangiri, C. (2012) Isolation and identification of endophytic bacteria of bananas (Musa spp.) in Kenya and their potential as biofertilizers for sustainable banana production. African Journal of Microbiology Research. [Online] 6 (34), 6414–6422. Available from: doi:10.5897/AJMR12.1170.

Nosrati, R., Owlia, P., Saderi, H., Rasooli, I. & Malboobi, M.A. (2014) Phosphate solubilization characteristics of efficient nitrogen fixing soil azotobacter strains. Iranian Journal of Mirobiology. 6 (4), 285–295.

Nursyamsi, D., Soepandi, D., Erfandi, S. & Widjaja, I.P.G. (1995) Penggunaan Bahan Organik, Pupuk P dan K untuk Peningkatan Produktivitas Tanah Podsolik. Risalah Seminar.

Puri, A., Padda, K.P. & Chanway, C.P. (2018) Nitrogen-fixation by endophytic bacteria in agricultural crops: recent advances. In: Amanullah, K. (ed.) Nitrogen in Agriculture - Updates. IntechOpen, pp.73–94.

Saito, A. & Minamisawa, K. (2006) Evaluation of the nitrogen-fixing ability of endophytic clostridia based on acetylene reduction and reverse transcription-PCR targeting the nifH transcript and ribosomal RNA. Microbes and Environments. [Online] 21 (1), 23–35. Available from: doi:10.1264/jsme2.21.23.

Santi, C., Bogusz, D. & Franche, C. (2013) Biological nitrogen fixation in non-legume plants. Annals of Botany. 111 (5), Oxford University Press, 743–767.

Setiawati, M.R., Arief, D.H., Suryatmana, P. & Hudaya, R. (2008) Aplikasi Bakteri Endofitik Penambat N2 untuk Meningkatkan Populasi Bakteri Endofitik dan Hasil Tanaman Padi Sawah. Jurnal Agrikultura. 19 (3), 13–19.

Silveira, A.P.D. da, Sala, V.M.R., Cardoso, E.J.B.N., Labanca, E.G. & Cipriano, M.A.P. (2016) Nitrogen metabolism and growth of wheat plant under diazotrophic endophytic bacteria inoculation. Applied Soil Ecology. [Online] 107, Elsevier B.V., 313–319. Available from: doi:10.1016/j.apsoil.2016.07.005.

Singh, R.K., Malik, N. & Singh, S. (2013) Improved nutrient use efficiency increases plant growth of rice with the use of IAA-overproducing strains of endophytic burkholderia cepacia Strain RRE25. Microbial Ecology. 66 (2), Springer, 375–384.

Uchida, R. (2000) Essential Nutrients for Plant Growth : Nutrient Functions and Deficiency Symptoms.In: Silva, J.A. & Uchida, R. (eds.) Plant Nutrient Management in Hawaii’s Soils, Approaches for Tropical and Subtropical Agriculture. College of Tropical Agriculture and Human Resources, Hawaii University, pp.31–55.

Waard, D.P.W.F. (1969) Foliar Diagnosis, Nutrition and Yield Stability of Black Pepper (Piper nigrum L.) in Sarawak. Wageningen University.

White, P.J. & Brown, P.H. (2010) Plant nutrition for sustainable development and global health. Annals of Botany. [Online] 102, 1073–1080. Available from: doi:10.1093/aob/mcq085.

Zaubin, R., Hidayat, A. & Sesda, M. (1995) Effect of NPK composition growth and health of black pepper. Jour. Spice and Med. Crop. 3, 51–55.




DOI: http://dx.doi.org/10.21082/jlittri.v25n2.2019.100-107

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