Responses of Selected Indonesian Rice Varieties under Excess Iron Condition in Media Culture at Seedling Stage

Yudhistira Nugraha, Sintho Wahyuning Ardie, Sintho Wahyuning Ardie, Indrastuti A. Rumanti, Indrastuti A. Rumanti, Suwarno Suwarno, Suwarno Suwarno, Munif Ghulammahdi, Munif Ghulammahdi, Hajrial Aswidinnoor, Hajrial Aswidinnoor

Abstract


Iron toxicity could limit rice productivity on irrigated lowland acid and swampy soil. The use of iron toxicity tolerant rice is an alternative strategy to improve rice productivity in these areas. We studied the phenotypic variation of twenty-four rice genotypes and characterized the fate of Fe2+ along its path between the roots and shoot of rice plant. Twenty-four rice genotypes form different agro-ecosystem were grown under agar nutrient solution conditions with 400 mg. L-1 iron stress and under normal condition. We found variation in the biomass accumulation of rice seedling during stress of iron namely, high accumulated biomass tolerant type and low accumulate biomass tolerant type. The relative biomass weight was highly correlated with the leaf bronzing scores (LBS) under excess iron. Based on these categorizations, we chose six genotypes to observe the present of Fe in root and shoot using invivo-staining 2,2 bypiridine. The result indicated that some genotypes were able to develop root and shoot aerenchym during iron stress. This was related to the development on root iron plaque and the iron content of the shoot of the rice seedling. In this present study, rice genotypes could be classified as the includer tolerant type (Inpara 2) and some others were the excluder tolerant type (Mahsuri, Pokkali and Siam Saba). This information on tolerance strategies is important for rice breeder to develop physiological-based breeding program of iron-toxicity tolerant in rice.

Keywords


excluder-type; includer-type; ferrous iron; aerenchym; biomass

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References


Asch, F., M. Becker, and D.S. Kpongor. 2005. A quick and efficient screen for resistance to iron toxicity in lowland rice. Journal of Plant Nutrition and Soil Science (168):764–773. doi:10.1002/jpln.200520540

Audebert, A. and K.L. Sahrawat. 2000 Mechanisms for iron toxicity tolerance in lowland rice. Journal of Plant Nutrition 23:1877–1885. doi: 10.1080/01904160009382150

Becana, M., J.F. Moran, and I Iturbe-Ormaetxe. 1998. Iron-dependent oxygen free radical generation in plants subjected to environmental stress: toxicity and antioxidant protection. Plant Soil. 201: 137–147. doi: 10.1023/a:1004375732137

Becker, M. and F. Asch. 2005. Iron toxicity in rice-conditions and management concepts. Journal of Plant Nutrition and Soil Science.168:558–573.doi: 10.1002/jpln.200520504

Briat, J.F., K Ravet, N. Arnaud, C. Duc, J. Boucherez, B. Touraine, F. Cellier, and F. Gaymard. 2010. New insights into ferritin synthesis and function highlight a link between iron homeostasis and oxidative stress in plants. Annals Botany. 105: 811–822. doi:10.1093/aob/mcp128

Chen, R.F., R.F. Shen, P. Gu, X.Y. Dong, C.W. Du, and J.F. Ma. 2006. Response of rice (Oryza sativa) with root surface iron plaque under aluminum stress. Annals Botany 98, 389–395

Colmer, T.D., 2003 Aerenchym and an inducible barrier to radial oxygen loss facilitate root aeration in upland, paddy and deep-water rice (Oryza sativa L.). Plant, Cell and Environment. 26: 301–309. doi: 10.1093/aob/mcf114

Dobermann, A. and T.H. Fairhurst. 2000. Nutrient Disorders and Nutrient Management. Manila. The International Rice Research Institute. p. 191.

Dufey, I, S. Gheysens, A Ingabire, and P. Bertin. 2014. Silicon application in cultivated rice (Oryza Sativa L and Oryza Glaberrima Steud) alleviates iron toxicity symptoms through the reduction in iron concentration in the leaf tissue. J Agron Crop Sci 200: 132–42.

Dufey, I., P. Hakizimana, X. Draye, S. Lutts, and P. Bertin. 2009. QTL mapping for biomass and physiological parameters linked to resistance mechanisms to ferrous iron toxicity in rice. Euphytica. 167: 143-160. doi:10.1007/s10681-008-9870-7

Engel, K., F. Asch, and M. Becker. 2012a. In vivo staining of reduced iron by 2,2′ bipyridine in rice exposed to iron toxicity. J Plant Nutr Soil Sci. 175:548-552. doi:10.1002/jpln.201200096

Engel, K., F. Asch, and M. Becker. 2012b. Classification of rice genotypes based on their tolerance and the mechanisms of adaptation to conditions of iron toxicity. J Plant Nutr Soil Sci. 175:871–881. doi:10.1002/jpln.201100421

Elec, V., C.A. Quimio, R. Mendoza, A.G. Sajise, S.E.J Beebout, G.B. Gregorio, and R.K. Singh. 2013. Maintaining elevated Fe2+ concentration in solution culture for the development of a rapid and repeatable screening technique for iron toxicity tolerance in rice (Oryza sativa L). Plant Soil doi: 10.1007/s11104-013-1739-4

Fageria, N.K., A.B. Santos, M.P.B Filho, and C.M. Guimarães. 2008. Iron toxicity in lowland rice. J Plant Nutri 31(9):1676-1697 doi: 10.1080/01904160802244902

Harahap, S.M., M. Ghulamahdi M, S.A. Aziz, and A. Sutandi. 2014. Relationship of ethylene production and aerenchym formation on oxidation ability and root surfaced-iron (Fe2+) accumulation under different iron concentrations and rice genotypes. Int. J. Appl. Sci. 4(1):186-194

Hollingsworth, R. G., Collins, T. P., Smith, V. E., and Nelson, S. C. 2011. Simple statistics for correlating survey responses. Journal of Extension 49 (5):14-21

Kotula, L., K. Ranathunge, L. Schreiber, and E. Steudle. 2009. Functional and chemical comparison of apoplastic barriers to radial oxygen loss in roots of rice (Oryza sativa L.) grown in aerated or deoxygenated solution. Journal Experimental Botany 60: 2155–2167.

Kopp, K. L., and Guillard, K. 2002. Relationship of turfgrass growth and quality to soil nitrate desorbed from anion exchange membranes. Crop Science (42):1232–1240.

Liu, J., C. Cao, M. Wong, Z. Zhang, and Y. Chai., 2010. Variations between rice cultivars in iron and manganese plaque on roots and the relation with plant cadmium uptake. Journal Environmental Science China 22: 1067–1072

Liu, W.J., Y.G. Zhu, F.A. Smith, and S.E. Smith. 2004. Do iron plaque and genotypes affect arsenate uptake and translocation by rice seedlings (Oryza sativa L.) grown in solution culture? Jornal Experimental Botany 55: 1707–1713

Mangiafico, S. S., Newman, J. P., Mochizuki, M. J., and Zurawski, D. 2008. Adoption of sustainable practices to protect and conserve water resources in container nurseries with greenhouse facilities. Acta Horticulturae (797):367–372.

Majerus, V., P. Bertin, and S. Lutts. 2009. Abscisic acid and oxidative stress implications in overall ferritin synthesis by African rice (Oryza glaberrima Steud.) seedlings exposed to short term iron toxicity. Plant Soil 324: 253–265. doi: 10.1007/s11104-009-9952-x

Majerus, V., Bertin P and Lutts S. 2007b. Effects of iron toxicity on osmotic potential, osmolytes and polyamines concentrations in the African rice (Oryza glaberrima Steud.). Plant Sci 173: 96–105. doi: 10.1016/j.plantsci.2007.04.003

Majerus V., P. Bertin, and V Swenden. 2007a. Organ-dependent responses of the African rice to short-term iron toxicity: Ferritin regulation and antioxidative responses. Biol Plant 51:303–312. doi: 10.1007/s10535-007-0060-6.

Masuda, H., Y. Ishimaru, M.S. Aung, T. Kobayashi, Y. Kakei, M. Takahashi, K. Higuchi, H. Nakanishi, and N.K Nishizawa. 2012. Iron biofortification in rice by the introduction of multiple genes involved in iron nutrition. Nature 2:543 DOI: 10.1038/srep00543

Nozoe, T., R. Agbisit, Y. Fukuta, R. Rodriguez, and S. Yanagihara. 2008: Characteristics of iron tolerant rice lines developed at IRRI under field conditions. JARQ: 42(3), 187–192.

Nugraha, Y., S.W. Ardie SW, Suwarno S, and H. Aswidinnoor H. 2016. Nutrient culture media with agar is effective for early- and rapid- screening of iron toxicity tolerant in rice. J Crop Sci Biotech. Doi: 10.1007/s12892-015-0075-z (Inpress)

Onaga, G., R. Edema, and G Asea. 2013. Tolerance of rice germplasm to iron toxicity stress and the relationship between tolerance, Fe2+, P and K content in the leaves and roots. Archives Agronomy Soil Science. 59(2):213-229. doi:10.1080/03650340.2011.622751

Prasetyo, T.B., F. Ahmad, and A. Saidi. 2013. Humic acid and water management to decrease Ferro (Fe2+) solution and increase productivity of established new rice field. J Trop Soil 17(1): 9-17. doi: 10.5400/jts.2012.17.1.9

Ramirez, L.M., N. Claassen, H. Werner, and A.M. Moawad. 2002 Effect of phosphorus, potassium and zinc fertilizers on iron toxicity in wetland rice (Oryza sativa L.). Plant Soil 239:197–206. doi: 10.1023/A:1015099422778

Ruskandar, A., T Rustiati and P. Wardana . 2011. Adopsi varietas unggul baru dan keuntungan usahatani padi di lahan rawa lebak. In: Seminar Nasional Hasil Penelitian Padi. Sukamandi (ID): Balai Besar Penelitian Padi.

Shimizu A., C.Q. Guerta, G.B. Gregorio, and H. Ikehashi. 2005. Improved mass screening of tolerance to iron toxicity in rice by lowering temperature of culture solution. Journal of Plant Nutrient. 28, 1481-1493. doi:10.1080/01904160500201352

Stephan, U.W. 2002. Intra- and intercellular iron trafficking and subcellular compartmentation within roots. Plant Soil, 241, 19–25.

Suhartini, T. 2004. Improvement of rice variety for Fe-toxicity soil. Bulletin Plasma Nutfah 10, 1-11 (Indonesian)

Thomine, S. and G. Vert. 2013. Iron transport in plants: better be safe than sorry. Current Opinion in Plant Biology 6:1–6

Thongbai, P., and B.A. Goodman. 2000. Oxidative free radicals generation and post-anoxic injury of rices (Oryza sativa L.) in an iron-toxic soil. Journal Plant Nutrition. 23, 1887–1900

Utami, D.W. and I.H. Somantri. 2014. Field evaluation and molecular identification of rice germplasms for Fe toxicity. J Agro Biogen 10: 9–17 (Indonesian).

Wu, L., M.Y. Shhadi, G. Gregorio, E. Matthus, M. Becker, and M. Frei. 2014. Genetic and physiological analysis of tolerance to acute iron toxicity in rice. Rice: 1–12. available at http://www.thericejournal.com/content/7/1/

Yoshida, S., D.A. Forno, J.H. Cock, and K.A. Gomez. 1976. Laboratory Manual for Physiological Studies of Rice. Manila.The International Rice Research Institute.p 1–9




DOI: http://dx.doi.org/10.21082/jpptp.v35n3.2016.p181-190

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