Penyisipan Gen OsDREB1A pada Tanaman Padi untuk Regenerasi Sifat Toleran Kekeringan

Budi Santosa, Sobir Sobir, Sriani Sujiprihati, Kurniawan Rudi Trijatmiko

Abstract


Genetic engineering is an alternative technology that can be used to assist breeders for improving the genetic potential of crop variety. Drought tolerant of rice was attempted by applying genetic engineering techniques by introducing gene OsDREB1A using Agrobacterium tumefaciens. The experiment was conducted in 2008-2010 at the laboratory and greenhouse of the Indonesian Center for Agriculture Biotechnology and Genetic Resources and Development, Bogor, using the transformation method. Callus derived from seeds of rice varieties Nipponbare was transformed by inserting the gene OsDREB1A, then regenerated and acclimatized to generate putative transgenic rice plants. The transformation of calli derived from 469 rice seeds of variety Nipponbare produced 103 putative transgenic rice plants. Plant heights of the putative transgenic plants obtained were generally lower than the control plants (88 cm). The numbers of rice panicles of the putative transgenic plants were generally less than the control plants (9 panicle). Production of filled grain of the putative transgenic plants were less than the control plants; three plants did not even produce seeds, namely B14(2)4.1a, D5.1, and D18. The results of the PCR analysis showed that all the putative transgenic rice plants were confirmed to carry the gene OsDREB1A.


Keywords


Transformation; regeneration; rice; gene OsDREB1A; drought tolerance

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DOI: http://dx.doi.org/10.21082/jpptp.v30n2.2011.p%25p

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


P-ISSN: 2541-5166
E-ISSN: 2541-5174
Terakreditasi No.646/AU3/P2MI-LIPI/2015 oleh Lembaga Ilmu Pengetahuan Indonesia


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