Metode Pengakaran Batang Bawah Mawar Bebas Prunus Necrotic Ringspot Virus Secara In Vitro

Erniawati Diningsih, Fitri Rahmawati, Yoyo Sulyo, - Darliah

Abstract


Metode untuk memproduksi batang bawah mawar bebas virus sudah diperoleh pada penelitian
sebelumnya. Untuk mendapatkan bibit bebas virus diperlukan metode pengakaran yang tepat secara in vitro. Pengakaran
merupakan salah satu tahap penting dalam teknik kultur jaringan untuk perbanyakan bibit tanaman secara cepat.
Penelitian bertujuan mendapatkan jenis media terbaik untuk pengakaran batang bawah mawar bebas virus. Penelitian
dilaksanakan di Laboratorium Virologi dan Kebun Percobaan Balai Penelitian Tanaman Hias, Segunung, Cianjur, Jawa
Barat (1.100 m dpl), dari bulan Januari sampai Desember 2003. Percobaan menggunakan rancangan acak kelompok
pola faktorial dengan 3 ulangan. Faktor pertama adalah 3 kultivar batang bawah mawar bebas virus (Rosa multiflora,
Rosa sp. cv. Multic, dan cv. Natal Brior). Faktor kedua adalah 7 komposisi media pengakaran (MS+IBA 0,5 mg/l,
MS+IBA 1,0 mg/l, MS+NAA 0,5 mg/l, MS+NAA 1,0 mg/l, MS+IAA 0,5 mg/l, MS+IAA 1,0 mg/l, dan MSO (tanpa
ZPT)). Hasil penelitian menunjukkan bahwa media pengakaran yang paling baik untuk memproduksi batang bawah
mawar bebas virus yaitu dengan komposisi MS+IBA 0,5 mg/ml. Implikasi hasil penelitian ini adalah tersedianya
teknologi pengakaran in vitro untuk batang bawah mawar

ABSTRACT. Diningsih, E., F. Rahmawati, Y. Sulyo, and Darliah. 2009. In Vitro Rooting Method for Producing
Free-Virus Rose Rootstock. The method for producing virus free plantlet of rose rootstock had been obtained in
previous study. In obtaining virus-free seed, availability of appropriate in vitro rooting method is needed. Rooting
is one of the important step in tissue culture technique for plant rapid multiplication. The purpose of this experiment
was to obtain the best rooting medium for producing virus-free rose rootstock. The experiment was conducted in the
Virology Laboratory of the Indonesian Ornamental Crop Research Institute, Segunung, Cianjur, West Java (1,100 m
asl.) from January to December 2003. The research was laid in a factorial randomized block design with 3 replications.
The first factor was 3 virus-free rose rootstock cultivars (Rosa multiflora, Rosa sp. cv. Multic, and cv. Natal Brior)
resulted from the previous experiment. The second factor was 7 compositions of medium (MS+IBA 0.5 mg/l, MS+IBA
1.0 mg/l, MS+NAA 0.5 mg/l, MS+NAA 1.0 mg/l, MS+IAA 0.5 mg/l, MS+IAA 1.0 mg/l, and MSO (without PGR)).
The results showed that the best rooting medium for producing virus-free rose rootstock was MS+IBA 0.5 mg/l.


Keywords


Rosa sp.; In vitro; Rootstock; Free virus PNRSV; IAA; IBA; NAA

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DOI: http://dx.doi.org/10.21082/jhort.v19n4.2009.p%25p

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