Studi Kultur Anther pada Aksesi Anthurium Lokal

Budi Winarto, N A Mattji

Abstract


Kultur anther merupakan salah satu terobosan teknologi dalam kultur jaringan untuk menyediakan
tanaman homozigot dalam waktu yang singkat. Aplikasi kultur anther pada aksesi Anthurium lokal dapat dilakukan
untuk menyediakan tetua homozigot yang tahan terhadap penyakit hawar daun. Penelitian bertujuan menguji respons
beberapa aksesi Anthurium lokal terseleksi dalam kultur anther dan kemampuan regenerasi kalus pada berbagai media
regenerasi. Penelitian dilakukan di Laboratorium dan Rumah Kaca Balai Penelitian Tanaman Hias dari bulan Januari
hingga Desember 2006. Anther diisolasi dari aksesi Anthurium lokal Cipanas Merah, Cipanas Putih, Cihideung-
Lembang Merah, Sukabumi Putih, dan Salatiga Putih. Hasil penelitian menunjukkan bahwa beberapa aksesi Anthurium
lokal memberikan respons yang berbeda terhadap kemampuan induksi kalus. Aksesi Cipanas Putih merupakan aksesi
yang paling responsif dibandingkan aksesi lainnya dengan persentase pembentukan kalus tertinggi (5,2%). Kalus yang
terbentuk dalam kultur anther memiliki kemampuan adaptasi yang baik terhadap inkubasi terang dan tumbuh lebih
cepat dibandingkan dengan aksesi yang lain. Kalus hasil kultur anther aksesi Cipanas Putih memiliki kemampuan
regenerasi yang tercepat (1,5-2,5 bulan) dan jumlah bakal tunas terbanyak (2,2-5,7 tunas). Media MMS-IIIR dan
MMS-TBN merupakan media yang sesuai untuk regenerasi tunas dari kalus hasil kultur anther aksesi Cipanas Putih.
Media tersebut juga sesuai untuk inisiasi kalus

ABSTRACT. Winarto, B. and N.A. Mattjik. 2009. Studies on Anther Culture of Local Accessions of Anthurium.
Anther culture is a technological breakthrough in tissue culture to provide homozygot plants in a shorter period
compared to conventional method. Anther culture applied for local Anthurium accession for getting desistant mother
plant against light blight. Anther culture application aimed to evaluate responses of anther culture of local accession
of Anthurium and capacity of callus regeneration derived from it, in different regeneration media. The study was
conducted at Tissue Culture Laboratory and Glasshouse of Indonesian Ornamental Crops Research Institute from
January to December 2006. Anthers of local accession of Cipanas Merah, Cipanas Putih, Cihideung-Lembang Merah,
Sukabumi Putih, and Salatiga Putih were used in this research. The results indicated that among local accessions of
Anthurium tested had different responses in their capacity to produce callus. Cipanas Putih accession was the most
responsive accession in callus formation (5.2%). Callus derived from this accession grew better and faster than other
accessions and easily adapted under light incubation. Callus resulted from anther culture of Cipanas Putih accession
exhibited faster regeneration (1.5-2.5 months) and produced highest number of shoots per callus (2.2-5.7 shoots).
The MMS-IIIR and MMS-TBN media were the appropriate media for shoot regeneration of callus produced from
anther culture of Cipanas Putih accession. The media was also suitable for callus initiation.


Keywords


Anthurium spp.; Local accession; Anther culture; In vitro media; Regeneration

Full Text:

PDF


DOI: http://dx.doi.org/10.21082/jhort.v19n4.2009.p%25p

Refbacks

  • There are currently no refbacks.
';



Copyright (c) 2013 Indonesian Center for Horticulture Research and Development

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 


Jurnal Hortikultura (J.Hort) has been indexed :

         

===================================================================================================================

 Creative Commons License
Junal Hortikultura is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Based on a work at http://ejurnal.litbang.pertanian.go.id/index.php/jhort
Permissions beyond the scope of this license may be available at www.litbang.pertanian.go.id.

Indonesian Center for Horticulture Research and Development

Jl. Tentara Pelajar No. 3C Kampus Penelitian Pertanian Cimanggu Bogor 16124, Indonesia
Telp.  +62 251-8372096, 7565366, (Ext. 324) (Hunting System)
Faks.  +62 251-8387651, 8575664, 8372096
E-mail: redaksi.jhorti@gmail.com

ISSN: 0853-7097
E-ISSN: 2502-5120

 

free web stats

View My Stats