Seleksi Tanaman Tomat Berdasarkan Ketahanan Pasif dan Aktif terhadap CMV

R Gaswanto, Nani Gunaeni, Ati Srie Duriat

Abstract


Tujuan penelitian ialah menentukan cara seleksi yang efektif ketahanan tanaman secara pasif maupun
aktif terhadap CMV untuk mendukung program pemuliaan tanaman tomat. Penelitian terdiri atas 2 tahap. Tahap
pertama dilakukan pada Juni-Desember 2001 dan tahap kedua dilakukan pada September 2002- Agustus 2003.
Penelitian dilakukan di Balai Penelitian Tanaman Sayuran Lembang (1.200 m dpl.). Hasil penelitian menunjukkan
bahwa seleksi ketahanan pasif tanaman tomat terhadap CMV efektif apabila diarahkan pada karakter morfologi daun.
Tanaman yang tahan CMV memiliki karakteristik jumlah daun yang sedikit, tetapi berbulu lebat dengan jumlah
stomata banyak. Seleksi ketahanan aktif tanaman tomat terhadap CMV efektif bila didasarkan pada indeks penyakit
secara visual yang ditunjang dengan teknik serologi melalui uji ELISA, karena tingginya peran gen aditif dalam
mengendalikan karakter konsentrasi virus

ABSTRACT. Gaswanto, R., N. Gunaeni, and A.S. Duriat. 2009. Tomato Selection Based on Passive and Active
Resistancy to CMV. The aim of this experiment was to determine the effective selection method either using active
or passive resistancy to CMV on tomato, for supporting tomato breeding program. The experiment was conducted
at Indonesian Vegetable Research Institute, Lembang consisted of 2 steps. The first step was conducted on June to
December 2001, while the second step was held on September 2002 to August 2003. The results showed that passive
resistance selection to CMV on tomato would be effective if it was focused on the characters of leaf morphology.
The plants resistant to CMV had fewer leaf number, dense leaf pubescence, and abundance of stomata numbers.
Meanwhile, active resistance selection would be effective if it was based on index disease symptom supported by
ELISA test, because of high role of additive gene in controlling virus concentration


Keywords


Lycopersicon esculentum; Cucumber mosaic virus; Heritability; Leaf morph type

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DOI: http://dx.doi.org/10.21082/jhort.v19n4.2009.p%25p

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