Regenerasi Kultur Lengkeng Dataran Rendah cv. Diamond River melalui Embriogenesis Somatik

Ika Roostika, V N Arief, N Sunarlim

Abstract


ABSTRAK. Beberapa kultivar lengkeng toleran dataran rendah telah diintroduksi ke Indonesia termasuk lengkeng
cv. Diamond River. Kultivar tersebut telah dibudidayakan secara komersial di daerah Kalimantan Barat. Namun,
pengembangannya menghadapi kendala dalam hal penyediaan bibit. Dalam rangka memperoleh bibit lengkeng
dalam jumlah yang berlimpah, perlu penerapan teknik kultur in vitro. Penelitian ini bertujuan untuk menginduksi
dan meregenerasikan kalus embriogenik lengkeng cv. Diamond River. Induksi kalus dilakukan menggunakan daun
muda sebagai eksplan. Regenerasi kalus embriogenik dilakukan dalam 4 tahap. Pada tahap pertama digunakan air
kelapa pada konsentrasi 5 dan 10%. Pada tahap kedua, diuji pengaruh auksin (IBA dan NAA) serta sitokinin (BA
dan kinetin) masing-masing pada taraf 0,5 ppm. Pada tahap ketiga, diuji pengaruh auksin IBA dan NAA pada taraf
0,1; 0,5; dan 1 ppm. Pada tahap keempat diuji perlakuan sukrosa pada taraf 2 dan 3% dengan atau tanpa auksin
(IBA dan NAA) masing-masing pada taraf 0,5 dan 1 ppm. Hasil penelitian menunjukkan bahwa regenerasi melalui
embriogenesis somatik berpeluang diterapkan pada tanaman lengkeng cv. Diamond River. Respons kalus embriogenik
lebih dominan ke arah pembentukan akar daripada tunas. Penggunaan media yang mengandung NAA 1 ppm mampu
meningkatkan pembentukan tunas hingga mencapai lebih dari 30%, sedangkan penggunaan sukrosa 3% tanpa auksin
mampu meningkatkan pembentukan planlet hingga mencapai 12%. Persentase keberhasilan aklimatisasi adalah
sebesar 14%.

ABSTRACT. Roostika, I., V.N. Arief, and N. Sunarlim. 2009. Regeneration of Lowland Longan cv. Diamond
River through Somatic Embryogenesis. Several low-land longan cultivars have been introduced to Indonesia,
including cultivar of Diamond River. This cultivar has been planted commercially and produced well in West
Kalimantan. Unfortunately, the development of this cultivar was facing a problem on the availability of planting
materials. In order to provide large number of Diamond River seedlings, tissue culture technique was used. The
aim of the study was to induce and regenerate embryogenic calli of longan cv. Diamond River. A research on callus
induction was conducted using young leaves as explants source. Regeneration of embryogenic calli was conducted
in 4 steps. The first, coconut water at the rate of 5 and 10% were used. The second, the auxin (IBA and NAA) and
cytokinin (BA and kinetin) at the level of 0.5 ppm, respectively were tested. The third, the IBA and NAA at the
level of 0.1, 0.5, and 1 ppm were used. The fourth, the sucrose at the level of 2 and 3% with or without addition of
IBA and NAA at the level of 0.5 and 1 ppm were used respectively. The results showed that somatic embryogenesis
regeneration was potentially applied to longan cv. Diamond River. The root formation was more dominant than the
shoot formation. The use of 1 ppm NAA could increase the shoot formation up to more than 30% whereas the use
of 3% sucrose without auxin could increase the plantlet formation up to 12%. The 14% of plantlet produced by this
technique grew well during acclimatization period.


Keywords


Euphoria longan Lam; Lowland; Regeneration; Somatic embryogenesis

Full Text:

PDF


DOI: http://dx.doi.org/10.21082/jhort.v20n1.2010.p%25p

Refbacks

  • There are currently no refbacks.
';



Copyright (c) 2013 Indonesian Center for Horticulture Research and Development

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 


Jurnal Hortikultura (J.Hort) has been indexed :

         

===================================================================================================================

 Creative Commons License
Junal Hortikultura is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Based on a work at http://ejurnal.litbang.pertanian.go.id/index.php/jhort
Permissions beyond the scope of this license may be available at www.litbang.pertanian.go.id.

Indonesian Center for Horticulture Research and Development

Jl. Tentara Pelajar No. 3C Kampus Penelitian Pertanian Cimanggu Bogor 16124, Indonesia
Telp.  +62 251-8372096, 7565366, (Ext. 324) (Hunting System)
Faks.  +62 251-8387651, 8575664, 8372096
E-mail: redaksi.jhorti@gmail.com

ISSN: 0853-7097
E-ISSN: 2502-5120

 

free web stats

View My Stats