Mapping of Resistance Genes to Brown Planthopper in Untup Rajab, an Indonesian Local Rice Variety

Muhamad Yunus, Diani Damayanti, Ahmad Dadang, Ahmad Warsun, Dani Satyawan, Kusumawaty Kusumanegara, Iswari Saraswati Dewi, Sutrisno Sutrisno, Bahagiawati Amir Husin

Abstract


Brown planthopper (BPH) is a major rice pest in Indonesia. The most economical and effective approach to control the insect pest is by using resistant varieties. Exploring for resistance genes is, therefore, a prerequisite for effective breeding program for BPH resistance. This study aimed to map BPH resistance genes in Untup Rajab, an Indonesian local rice variety. Genetic map was constructed using an F2 population from a cross between TN-1 and Untup Rajab, and SNP markers from RiceLD SNP Chip. Phenotyping was performed using bulk seedling test on F2:3 seedlings against two BPH populations, i.e. X1 and S1. Four QTLs
were identified on chromosomes 5, 6, 8, and 11 with PVE values of 7.63%, 9.40%, 17.66%, and 3.05%, respectively. Relatively normal distribution of resistance phenotype and the relatively low PVE values indicate that Untup Rajab has a quantitative resistance to BPH with two different resistance loci identified for each BPH test population. The QTL on chromosome 8 overlaps with OsHI-LOX gene, which is associated with resistance to BPH, and adjacent to another QTL for resistance to green leafhopper. The QTL on chromosome 6 was found near OsPLDα4 and OsPLDα5 genes which are related to BPH resistance. Meanwhile, the QTL intervals on chromosome 5 and 11 did not overlap with any known BPH QTLs or genes, which make them attractive candidates for novel BPH resistance gene discovery.


Keywords


Brown planthopper; rice; resistance gene; local rice variety.

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References


Baehaki, S.E. (2010) Perubahan biotipe wereng cokelat

pada beberapa sentra produksi padi di Indonesia.

Dalam: Prosiding Seminar Nasional V, Pemberdayaan

Keanekaragaman Serangga untuk Peningkatan

Kesejahteraan Masyarakat. Perhimpunan Entomologi

Indonesia. hlm. 53–62.

Baehaki, S.E. (2012) Standar operasional prosedur

pengujian galur dan varietas padi terhadap wereng

batang cokelat (Nilaparvata lugens). Jakarta, Badan

Penelitian dan Pengembangan .

Baehaki, S.E. & Iswanto, E.H. (2017) The filtering of rice

resistance and population buildup to determine

antibiosis and tolerance as characteristics of rice

resistance to brown planthopper biotype 3. American

Journal of Engineering Research. [Online] 6 (3), 188–

Available from: http://www.ajer.org/papers/

v6(03)/ZE0603188196.pdf [Accessed 28 June 2018].

Bhanu, K.V., Lakshmi, V.J., Katti, G. & Reddy, A.V. (2014)

Antibiosis and tolerance mechanisms of resistance in

rice varieties carrying brown planthopper resistance

genes. Asian Journal of Biological and Life Sciences, 3

(2), 108–113.

Brar, D.S., Virk, P.S., Jena, K.K. & Khush, G.S. (2009)

Breeding for resistance to planthoppers in rice. In:

Heong, K.L. & Hardy, B. (eds.) Planthoppers: New

threats to the sustainability of intensive rice production

systems in Asia. International Rice Research Institute

Los Baños, Philippines, 401–409.

Chaerani, Damayanti, D., Trisnaningsih, Yuriyah, S.,

Kusumanegara, K., Dadang, A., Sutrisno &

Bahagiawati (2016) Virulence of brown planthopper

and development of core collection of the pest. Jurnal

Penelitian Tanaman Pangan, 35 (2), 109–118.

Dellaporta, S., Wood, J. & Hicks, J. (1983) A plant DNA

minipreparation: Version II. Plant Molecular Biology

Reporter, 1, 19–21.

Du, B., Zhang, W., Liu, B., Hu, J., Wei, Z., Shi, Z., He, R.,

Zhu, L., Chen, R., Han, B. & He, G. (2009)

Identification and characterization of Bph14, a gene

conferring resistance to brown planthopper in rice.

Proceedings of the National Academy of Sciences of

the United States of America. [Online] 106 (52),

–22168. Available from: doi:10.1073/

pnas.0912139106 [Accessed 28 June 2018].

Guo, J., Xu, C.,Wu, D., Zhao, Y., Qiu, Y., Wang, X.,

Ouyang, Y., Cai, B., Liu, X., Jing, S., Shangguan, X.,

Wang, H., Ma, Y., Hu, L., Wu, Y., Shi, S., Wang, W.,

Zhu, L., Xu, X., Chen, R., Feng, Y., Du, B. & He, G.

(2018) Bph6 encodes an exocyst-localized protein and

confers broad resistance to planthoppers in rice.

Nature Genetics. [Online] 50 (2), 297–306. Available

from: doi:10.1038/s41588-018-0039-6 [Accessed 10

June 2018].

Habibuddin, H. (1989) Variation of brown planthopper

population from major rice regions of Peninsular

Malaysia. MARDI Research Journal, 17 (2), 218–224.

Heinrichs, E.A. (1986) Perspectives and directions for the

continued development of insect-resistant rice

varieties. Agriculture, Ecosystems & Environment.

[Online] 18 (1), 9–36. Available from: doi:10.1016/

-8809(86)90172-6 [Accessed 22 June 2018].

Hu, J., Chang, X., Zou, L., Tang, W. & Wu, W. (2018)

Identification and fine mapping of Bph33, a new brown

planthopper resistance gene in rice (Oryza sativa L.).

Rice. [Online] 11 (1), 55. Available from: doi:10.1186/

s12284-018-0249-7 [Accessed 6 November 2018].

Jairin, J., Phengrat, K., Teangdeerith, S., Vanavichit, A. &

Toojinda, T. (2006) Mapping of a broad-spectrum

brown planthopper resistance gene, Bph3, on rice

chromosome 6. Molecular Breeding. [Online] 19 (1),

–44. Available from: doi:10.1007/s11032-006-9040-

[Accessed 26 June 2018].

Jena, K.K., Jeung, J.U., Lee, J.H., Choi, H.C. & Brar, D.S.

(2006) High-resolution mapping of a new brown

planthopper (BPH) resistance gene, Bph18(t), and

marker-assisted selection for BPH resistance in rice

(Oryza sativa L.). Theoretical and Applied Genetics.

[Online] 112, 288–297. Available from: doi:10.1007/

s00122-005-0127-8 [Accessed 26 June 2018].

Jena, K.S., Hechanova, S.L., Verdeprado, H., Prahalada,

G.D. & Kim, S.R. (2017) Development of 25 nearisogenic

lines (NILs) with ten BPH resistance genes in

rice (Oryza sativa L.): Production, resistance spectrum,

and molecular analysis. Theoretical and Applied

Genetics. [Online] 130 (11), 2345–2360. Available

from: doi:10.1007/s00122-017-2963-8 [Accessed 26

June 2018].

Kawahara, Y., de la Bastide, M., Hamilton, J.P., Kanamori,

H., McCombie, W.R., Ouyang, S., Schwartz, D.C.,

Tanaka, T., Wu, J., Zhou, S. & Childs, K.L. (2013)

Improvement of the Oryza sativa Nipponbare reference

genome using next generation sequence and optical

map data. Rice. [Online] 6 (1), 4. Available from:

doi:10.1186/1939-8433-6-4 [Accessed 22 October

.

Kumari, S., Sheba, J.M., Marappan, M., Ponnuswamy, S.,

Seetharaman, S., Pothi, N., Subbarayalu, M.,

Muthurajan, R. & Natesa, S. (2010) Screening of IR50

× Rathu Heenati F7 RILs and identification of SSR

markers linked to brown planthopper (Nilaparvata

lugens Stål) resistance in rice (Oryza sativa L.).

Molecular Biotechnology, 46, 63–71.

Liu, Y., Su, C., Jiang, L., He, J., Wu, H., Peng, C. & Wan, J.

(2009) The distribution and identification of brown

planthopper resistance genes in rice. Hereditas.

[Online] 146 (2), 67–73. Available from:

doi:10.1111/j.1601-5223.2009.02088.x [Accessed 22

October 2018].

Liu, Y., Wu, H., Chen, H., Liu, Y., He, J., Kang, H., Sun, Z.,

Pan, G., Wang, Q., Hu, J., Zhou, F., Zhou, K., Zheng,

X., Ren, Y., Chen, L., Wang, Y., Zhao, Z., Lin, Q., Wu,

F., Zhang, X., Guo, X., Cheng, X., Jiang, L., Wu, C.,

Wang, H. & Wan, J. (2015) A gene cluster encoding

lectin receptor kinases confers broad-spectrum and

durable insect resistance in rice. Nature

Biotechnology. [Online] 33 (3), 301–307. Available

from: doi:10.1038/nbt.3069 [Accessed 26 June 2018].

Madden, L.V., Hughes, G. & van den Bosch, F. (2017)

Chapter 4: Temporal analysis I: Quantifying and

comparing epidemics. In: Madden, L.V., Hughes, G. &

van den Bosch, F. (eds.) The Study of Plant Disease

Epidemics. [Online] 63–116. Available from:

doi:10.1094/9780890545058.004 [Accessed 21 June

.

Meng, L., Li, H., Zhang, L. & Wang, J. (2015) QTL

IciMapping: Integrated software for genetic linkage

map construction and quantitative trait locus mapping

in biparental populations. The Crop Journal. [Online] 3

(3), 269–283. Available from:

doi:10.1016/j.cj.2015.01.001 [Accessed 22 September

.

Myint, K.K.M., Fujita, D., Matsumura, M., Sonoda, T.,

Yoshimura, A. & Yasui, H. (2012) Mapping and

pyramiding of two major genes for resistance to the

brown planthopper (Nilaparvata lugens [Stål]) in the

rice cultivar ADR52. Theoretical and Applied Genetics.

[Online] 124 (3), 495–504. Available from:

doi:10.1007/s00122-011-1723-4 [Accessed 22

September 2018].

Oka, I.N. & Bahagiawati, A.H. (1983) Wereng coklat dan

pengendaliannya dalam prespektif. Masalah dan hasil

penelitian padi. Dalam: Risalah Lokakarya Penelitian

Padi. Cibogo, Bogor, 22–24 Maret 1983. hlm. 87–101.

Oka, I.N. & Bahagiawati, A.H. (1984) Development and

management of a new brown planthopper (Nilaparvata

lugens Stål) biotype in North Sumatra, Indonesia.

[Online] Central Research Institute for Food Crops.

Bogor, Indonesia. Contribution 71, 14 pp. Available

from: https://www.cabdirect.org/cabdirect/abstract/

[Accessed 22 September 2018].

Qi, J., Zhou, G., Yang, L., Erb, M., Lu, Y., Sun, X., Cheng, J.

& Lou, Y. (2011) The chloroplast-localized

phospholipases D4 and 5 regulate herbivoreinduced

direct and indirect defenses in rice. Plant

Physiology. [Online] 157 (4), 1987–1999. Available

from: doi:10.1104/pp.111.183749 [Accessed 22

November 2018].

Qiu, Y., Guo, J., Jing, S., Tang, T, Zhu, L. & He, G. (2011)

Identification of antibiosis and tolerance in rice

varieties carrying brown planthopper resistance genes.

Entomologia Experimentalis et Applicata. [Online] 141

(3), 224–231. Available from: doi:10.1111/j.1570-

2011.01192.x [Accessed 10 December 2018].

Santhanalakshmi, S., Saikumar, S., Rao, S., SaiHarini, A.,

Khera, P., Shashidar, H.E. & Kardivel, P. (2010)

Mapping genetic locus linked to BPH resistance in rice.

International Journal of Plant Breeding and Genetics, 4

(1), 13–22.

Somantri, I.H. (1998) Hama wereng coklat padi:

Perkembangan biotipe, mekanisme dan genetika

ketahanan varietas. Buletin Agrobio, 2 (1), 36–44.

Sun, L., Su, S., Wang, C., Zhai, H. & Wan, J. (2005)

Mapping of a major resistance gene to the brown

planthopper in the rice cultivar Rathu Heenati.

Breeding Science. [Online] 55 (4), 391–396. Available

from: doi:10.1270/jsbbs.55.391 [Accessed 26 June

.

Hu, J., Chang, X., Zou, L., Tang, W. & Wu, W. (2018)

Identification and fine mapping of Bph33, a new brown

planthopper resistance gene in rice (Oryza sativa L.).

Rice. [Online] 11 (1), 55. Available from: doi:10.1186/

s12284-018-0249-7 [Accessed 6 November 2018].

Jairin, J., Phengrat, K., Teangdeerith, S., Vanavichit, A. &

Toojinda, T. (2006) Mapping of a broad-spectrum

brown planthopper resistance gene, Bph3, on rice

chromosome 6. Molecular Breeding. [Online] 19 (1),

–44. Available from: doi:10.1007/s11032-006-9040-

[Accessed 26 June 2018].

Jena, K.K., Jeung, J.U., Lee, J.H., Choi, H.C. & Brar, D.S.

(2006) High-resolution mapping of a new brown

planthopper (BPH) resistance gene, Bph18(t), and

marker-assisted selection for BPH resistance in rice

(Oryza sativa L.). Theoretical and Applied Genetics.

[Online] 112, 288–297. Available from: doi:10.1007/

s00122-005-0127-8 [Accessed 26 June 2018].

Jena, K.S., Hechanova, S.L., Verdeprado, H., Prahalada,

G.D. & Kim, S.R. (2017) Development of 25 nearisogenic

lines (NILs) with ten BPH resistance genes in

rice (Oryza sativa L.): Production, resistance spectrum,

and molecular analysis. Theoretical and Applied

Genetics. [Online] 130 (11), 2345–2360. Available

from: doi:10.1007/s00122-017-2963-8 [Accessed 26

June 2018].

Kawahara, Y., de la Bastide, M., Hamilton, J.P., Kanamori,

H., McCombie, W.R., Ouyang, S., Schwartz, D.C.,

Tanaka, T., Wu, J., Zhou, S. & Childs, K.L. (2013)

Improvement of the Oryza sativa Nipponbare reference

genome using next generation sequence and optical

map data. Rice. [Online] 6 (1), 4. Available from:

doi:10.1186/1939-8433-6-4 [Accessed 22 October

.

Kumari, S., Sheba, J.M., Marappan, M., Ponnuswamy, S.,

Seetharaman, S., Pothi, N., Subbarayalu, M.,

Muthurajan, R. & Natesa, S. (2010) Screening of IR50

× Rathu Heenati F7 RILs and identification of SSR

markers linked to brown planthopper (Nilaparvata

lugens Stål) resistance in rice (Oryza sativa L.).

Molecular Biotechnology, 46, 63–71.

Liu, Y., Su, C., Jiang, L., He, J., Wu, H., Peng, C. & Wan, J.

(2009) The distribution and identification of brown

planthopper resistance genes in rice. Hereditas.

[Online] 146 (2), 67–73. Available from:

doi:10.1111/j.1601-5223.2009.02088.x [Accessed 22

October 2018].

Liu, Y., Wu, H., Chen, H., Liu, Y., He, J., Kang, H., Sun, Z.,

Pan, G., Wang, Q., Hu, J., Zhou, F., Zhou, K., Zheng,

X., Ren, Y., Chen, L., Wang, Y., Zhao, Z., Lin, Q., Wu,

F., Zhang, X., Guo, X., Cheng, X., Jiang, L., Wu, C.,

Wang, H. & Wan, J. (2015) A gene cluster encoding

lectin receptor kinases confers broad-spectrum and

durable insect resistance in rice. Nature

Biotechnology. [Online] 33 (3), 301–307. Available

from: doi:10.1038/nbt.3069 [Accessed 26 June 2018].

Madden, L.V., Hughes, G. & van den Bosch, F. (2017)

Chapter 4: Temporal analysis I: Quantifying and

comparing epidemics. In: Madden, L.V., Hughes, G. &

van den Bosch, F. (eds.) The Study of Plant Disease

Epidemics. [Online] 63–116. Available from:

doi:10.1094/9780890545058.004 [Accessed 21 June

.

Meng, L., Li, H., Zhang, L. & Wang, J. (2015) QTL

IciMapping: Integrated software for genetic linkage

map construction and quantitative trait locus mapping

in biparental populations. The Crop Journal. [Online] 3

(3), 269–283. Available from:

doi:10.1016/j.cj.2015.01.001 [Accessed 22 September

.

Myint, K.K.M., Fujita, D., Matsumura, M., Sonoda, T.,

Yoshimura, A. & Yasui, H. (2012) Mapping and

pyramiding of two major genes for resistance to the

brown planthopper (Nilaparvata lugens [Stål]) in the

rice cultivar ADR52. Theoretical and Applied Genetics.

[Online] 124 (3), 495–504. Available from:

doi:10.1007/s00122-011-1723-4 [Accessed 22

September 2018].

Oka, I.N. & Bahagiawati, A.H. (1983) Wereng coklat dan

pengendaliannya dalam prespektif. Masalah dan hasil

penelitian padi. Dalam: Risalah Lokakarya Penelitian

Padi. Cibogo, Bogor, 22–24 Maret 1983. hlm. 87–101.

Oka, I.N. & Bahagiawati, A.H. (1984) Development and

management of a new brown planthopper (Nilaparvata

lugens Stål) biotype in North Sumatra, Indonesia.

[Online] Central Research Institute for Food Crops.

Bogor, Indonesia. Contribution 71, 14 pp. Available

from: https://www.cabdirect.org/cabdirect/abstract/

[Accessed 22 September 2018].

Qi, J., Zhou, G., Yang, L., Erb, M., Lu, Y., Sun, X., Cheng, J.

& Lou, Y. (2011) The chloroplast-localized

phospholipases D4 and 5 regulate herbivoreinduced

direct and indirect defenses in rice. Plant

Physiology. [Online] 157 (4), 1987–1999. Available

from: doi:10.1104/pp.111.183749 [Accessed 22

November 2018].

Qiu, Y., Guo, J., Jing, S., Tang, T, Zhu, L. & He, G. (2011)

Identification of antibiosis and tolerance in rice

varieties carrying brown planthopper resistance genes.

Entomologia Experimentalis et Applicata. [Online] 141

(3), 224–231. Available from: doi:10.1111/j.1570-

2011.01192.x [Accessed 10 December 2018].

Santhanalakshmi, S., Saikumar, S., Rao, S., SaiHarini, A.,

Khera, P., Shashidar, H.E. & Kardivel, P. (2010)

Mapping genetic locus linked to BPH resistance in rice.

International Journal of Plant Breeding and Genetics, 4

(1), 13–22.

Somantri, I.H. (1998) Hama wereng coklat padi:

Perkembangan biotipe, mekanisme dan genetika

ketahanan varietas. Buletin Agrobio, 2 (1), 36–44.

Sun, L., Su, S., Wang, C., Zhai, H. & Wan, J. (2005)

Mapping of a major resistance gene to the brown

planthopper in the rice cultivar Rathu Heenati.

Breeding Science. [Online] 55 (4), 391–396. Available

from: doi:10.1270/jsbbs.55.391 [Accessed 26 June

.




DOI: http://dx.doi.org/10.21082/jbio.v14n2.2018.p75-84

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