Effect of Introgression of Pup1 Locus on Rice Seedling under Phosphorus Deficiency

Nurul Hidayatun, Joko Prasetiyono

Abstract


The lack of soil-phosphorus (P) element will result in plant growth retardation. Plants could survive in P deficiency stress by increasing the ability of P uptake or by increasing the efficiency in the P utilization. The aims of this study were to understand genetic composition of rice genotypes possessing Pup1 locus and to know root and leaf growth responses at different P availability condition. The three rice genotypes (IR74, IR74-Pup1, and Kasalath) were analyzed for their genetic composition using SNP markers. The phenotypic experiment was arranged using a Completely Randomized Design with four replications and performed hydroponically in nutrient solution with different availability of P. The result showed that IR74-Pup1 had 84.4% similarities to its parent (IR74) with 13.6% of donor segments, where the Pup1 locus located. The influence of Pup1 locus
introgression on total length, surface area, diameter, and volume of the root varied at each growth stage. IR74 and IR74-Pup1 had root and leaf growth restriction on low P, but Pup1 locus introgression showed better growth performance, both in normal P and in low P conditions. The introgression of Pup1 locus increases plant ability to reduce the impact of growth inhibition
caused by P deficiency. 


Keywords


Rice; P deficiency; Pup1 locus; hydroponic screening; SNP marker

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References


Anonim (1999) Phosphorus deficiency symptoms in some

crops. Better Crops, 83 (1), 20–23.

Carstensen, A., Herdean, A., Schmidt, S.B., Sharma, A.,

Spetea, C., Pribil, M. & Husted, S. (2018) The impacts

of phosphorus deficiency on the photosynthetic

electron transport chain. Plant Physiology, 177, 271–

Chen, H., Xie, W., He, H., Yu, H., Chen, W., Li, J., Yu, R.,

Yao, Y., Zhang, W., He, Y., Tang, X., Zhou, F., Deng,

X.W. & Zhang, Q. (2014) A high-density SNP

genotyping array for rice biology and molecular

breeding. Molecular Plant, 7 (3), 541–553.

Chin, H.J., Lu, X., Haefele, S.M., Gamuyao, R., Ismail, A.M.,

Wissuwa, M. & Heuer, S. (2010) Development and

application of gene-based markers for the major rice

QTL. Phosphorus uptake 1. Theoritical and Applied

Genetics, 120 (6), 1073–1086.

Chin, J.H., Gamuyao, R., Dalid, C., Bustamam, M.,

Prasetiyono, J., Moeljopawiro, S., Wissuwa, M. &

Heuer, S. (2011) Developing rice with high yield under

phosphorus deficiency: Pup1. Sequence to Application

Plant Physiology, 156, 1202–1216.

Famoso, A.N., Clark, R.T., Shaff, J.E., Craft, E., McCouch,

S.R. & Kochian, L.V. (2010) Development of a novel

aluminum tolerance phenotyping platform used for

comparisons of cereal aluminum tolerance and

investigations into rice. Plant Physiology, 153, 1678–

Fan, Y., Chen, J. & Wu, J. (2009) Avoidance of linkage drag

between blast resistance gene and the QTL

conditioning spikelet fertility based on genotype

selection against heading date in rice. Rice Science,

(1), 21–26.

Gamuyao, R., Chin, J.H., Tanaka, J.P., Pesaresi, P.,

Catausan, S., Dalid, C., Loedin, I.S., Mendoza, E.M.T., Wissuwa, M. & Heuer, S. (2012) The protein kinase

OsPSTOL1 from traditional rice confers tolerance of

phosphorus deficiency. Nature, 488, 535–539.

Heuer, S., Lu, X., Chin, J.H., Tanaka, J.P., Kanamori, H.,

Matsumoto, T., De Leon, T., Ulat, V.J., Ismail, A.M.,

Yano, M. & Wissuwa, M. (2009) Comparative

sequence analyses of the major quantitative trait locus

Phosphorus uptake 1 (Pup1) reveal a complex genetic

structure. Plant Biotechnology, 7, 456–471.

Julia, C., Wissuwa, M., Kretzschmar, T., Jeong, K. & Rose,

T. (2016) Phosphorus uptake, partitioning, and

redistribution during grain filling in rice. Annals of

Botany, 118, 1151–1162.

Mammadov, J., Aggarwal, R., Buyyarapu, R. & Kumpatla, S.

(2012) SNP markers and their impact on plant

breeding. International Journal of Plant Genomics,

doi: 10.1155/2012/728398.

McCouch, S.R., Zhao, K., Wright, M., Tung, C.W., Ebana,

K., Thomson, M., Reynolds, A., Wang, D., DeClerck,

G., Ali, Md.L., McClung, A., Eizenga, G. & Bustamante,

C. (2010) Development of genome-wide SNP assays

for rice. Breeding Science, 60, 524–535.

Ozturk, L., Eker, S., Torun, B. & Cakmak, I. (2005) Variation

in phosphorus efficiency among 73 bread and durum

wheat genotypes grown in a phosphorus-deficient

calcareous soil. Plant and Soil, 269, 69–80.

Pallotta, M., Langridge, P. & Barker, S.J. (2000) RFLP

mapping of manganese efficiency in barley.

Theoretical and Applied Genetics, 101 (7), 1100–1108.

Pariasca, J., Chin, J.H., Dramé, K.N., Dalid, C., Heuer, S. &

Wissuwa, M. (2014) A novel allele of the P-starvation

tolerance gene OsPSTOL1 from African rice (Oryza

glaberrima Steud.) and its distribution in the genus

Oryza. Theoretical and Applied Genetics, 127, 1387–

Prasetiyono, J., Aswidinnoor, H., Moeljopawiro, S.,

Sopandie, D. & Bustamam, M. (2008) Identifikasi

marka polimorfik untuk pemuliaan padi toleran

defisiensi fosfor. Jurnal AgroBiogen, 4 (2), 51–58.

Prasetiyono, J., Suhartini, T., Soemantri, I.H., Tasliah,

Moeljopawiro, S., Aswidinnoor, H., Sopandie, D. &

Bustaman, M. (2012) Evaluasi beberapa galur-Pup1

tanaman padi (Oryza sativa L.) pada larutan hara dan

lapangan. Jurnal Agronomi Indonesia, 40 (2), 83–90.

Prasetiyono, J., Tasliah, Ma’sumah, Hidayatun, N.,

Suhartini, T. & Soemantri, I.H. (2016) Analisis

molekuler dan uji daya hasil galur-galur BC2F8 padi

Pup1. Jurnal AgroBiogen, 12 (1), 1–10.

Rose, T.J., Impa, S.M., Rose, M.T., Pariasca-Tanaka, J.,

Mori, A., Heuer, S., Johnson-Beebout, S.E. &

Wissuwa, M. (2013) Enhancing phosphorus and zinc

acquisition efficiency in rice: A critical review. Annual

Botany, 112, 331–345.

Shavrukov, Y., Genc, Y. & Hayes, J. (2012) The use of

hydroponics in abiotic stress tolerance research. In:

Asao, T. (ed.) Hydroponics: A standard methodology

for plant biological researches. [e-book] InTech,

Rijeka, Croatia, pp. 39–66. Available from: https://

www.intechopen.com/books/hydroponics-a-standardmethodology-

for-plant-biological-researches/the-useof-

hydroponics-in-abiotic-stress-tolerance-research

[Accessed 12 Januari 2013].

Singh, N., Jayaswal, P.K., Panda, K., Mandal, P., Kumar,

V., Singh, B., Mishra, S., Singh, Y., Singh, R., Rai, V.,

Gupta, A., Sharma, T.R. & Singh, N.K. (2015) Singlecopy

gene based 50K SNP chip for genetic studies

and molecular breeding in rice. Scientific Reports, 5,

doi: 10.1371/journal.pone.0084136.

Sun, Y. (2018) Identification of nitrogen, phosphorus, and

potassium deficiencies based on temporal dynamics of

leaf morphology and color. Sustainability, 10 (3), 762.

doi: 10.3390/su10030762.

Tasma, I.M. (2014) Single nucleotide polymorphism (SNP)

sebagai marka DNA masa depan. WartaBiogen, 10

(3), 7–10.

Thomson, M.J., Mcnally, K.L. & McClung, A. (2012) Highthroughput

single nucleotide polymorphism genotyping

for breeding applications in rice using the BeadXpress

platform. Molecular Breeding, 29 (4), 875–886.

Vinod, K.K. & Heuer, S. (2012) Approaches towards

nitrogen- and phosphorus-efficient rice. AoB Plants, 1–

doi: 10.1093/aobpla/pls028.

Wissuwa, M., Wegner, J., Ae, N. & Yano, M. (2002)

Substitution mapping of Pup1: A major QTL increasing

phosphorus uptake of rice from a phosphorus-deficient

soil. Theoretical and Applied Genetics, 105, 890–897.

Wissuwa, M. (2003) How do plants achieve tolerance to

phosphorus deficiency? Small causes with big effects.

Plant Physiology, 133, 1947–1958.

Wissuwa, M. & Ae, N. (2001) Further characterization of two

QTLs that increase phosphorus uptake of rice (Oryza

sativa L.) under phosphorus deficiency. Plant and Soil,

, 275–286.

Wissuwa, M., Yano, M. & Ae, N. (1998) Mapping of QTLs

for phosphorus-deficiency tolerance in rice (Oryza

sativa L.). Theoritical and Applied Genetics, 97, 777–

Xu, H.X., Weng, X.Y. & Yang, Y. (2007) Effect of

phosphorus deficiency on the photosynthetic

characteristics of rice plants. Russian Journal of Plant

Physiology, 54 (6), 741–748.

Yong-fu, L., An-Cheng, L., Hassan, M.J. & Xing-Hua, W.

(2006) Effect of phosphorus deficiency on leaf

photosynthesis and carbon partitioning in two rice

genotypes with contrasting low phosphorus

susceptibility. Rice Science, 13 (4), 283–290.

Yoshida, S., Forno, D.A., Cock, J.H. & Gomez, K.A. (1976)

Laboratory manual for physiological studies of rice.

Los Baños, Philippines, International Rice Research

Institute.




DOI: http://dx.doi.org/10.21082/jbio.v14n2.2018.p97-104

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