Potensi Quorum Quencher Bakteri Filosfer dan Rizosfer terhadap Dickeya dadantii

Taruna D. Satwika, Iman Rusmana, Alina Akhdiya

Abstract


Ekspresi gen-gen virulensi pada Dickeya dadantii diatur oleh proses quorum sensing menggunakan asil-homoserin lakton (AHL) sebagai molekul sinyal. Patogenisitas bakteri tersebut dapat dihambat oleh aktivitas quorum quenching (QQ) bakteribakteri penghasil AHL-laktonase. Tujuan penelitian ini adalah mengisolasi dan mengarakterisasi bakteri penghasil AHLlaktonase asal rizosfer dan filosfer yang berpotensi untuk dikembangkan sebagai quorum quencher untuk D. dadantii. Isolasi bakteri dilakukan dari sampel daun dan sampel tanah rizosfer beberapa komoditas tanaman asal Sukabumi, Tegal, Kupang, dan Wonosobo. Sebanyak 8 dari 79 isolat bakteri yang diperoleh menunjukkan aktivitas QQ terhadap bioindikator Chromobacterium violaceum. Bioasai respons hipersensitif (hypersensitive response) yang dilakukan pada tanaman tembakau
menunjukkan enam (KT2, KT9, KT10, KUT1, TKF2, and WKF3) dari delapan isolat tersebut tidak menimbulkan respons hipersensitif. Keenam isolat tersebut mampu menekan virulensi D. dadantii pada umbi kentang. Sekuen 16S rRNA enam isolat tersebut memiliki kemiripan tertinggi dengan Bacillus cereus, B. aryabhattai, B. acidiceler, dan Micrococcus aloeverae. B. cereus KT9 and B. aryabhattai TKF2 terdeteksi memiliki gen penyandi AHL-laktonase (aiiA). Ini merupakan laporan yang pertama tentang aktivitas QQ pada spesies M. aloeverae, B. aryabhattai, and B. acidiceler. Keberadaan gen aiiA pada B.
aryabhattai juga belum pernah dilaporkan sebelumnya. Penelitian ini memberikan informasi baru tentang aktivitas QQ ketiga isolat tersebut dan potensinya sebagai quorum quencher untuk D. dadantii.


Keywords


AHL laktonase; biokontrol; Dickeya dadantii; quorum quenching

Full Text:

PDF

References


Akhdiya, A., D. Sukmadjaja, I. Rusmana. 2017. Bioprospeksi Bacillus spp. penghasil AHL-laktonase sebagai biokontrol bakteri fitopatogen. Dalam : Wihardjaka, A., A. Dariah, E.S. Harsanti, H. Lina, A. Hasyim. Prosiding Seminar Nasional Inovasi Pestisida Ramah Lingkungan Mendukung Swasembada Pangan. Pati, 6-7 September 2017. Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian, Badan Penelitian dan Pengembangan Pertanian.

Ban, H., Chai, X., Lin, Y., Zhou, Y., Peng, D., Zhou, Y., Zou, Y., Yu, Z. and Sun, M. 2009. Transgenic Amorphophallus konjac expressing synthesized acylhomoserine lactonase (aiiA) gene exhibit enhanced resistance to soft rot disease. Plant Cell Report 28:1847-185

Chan, K. G., S. Atkinson, K. Mathee, C.K. Sam, S.R. Chhabra, M. Cámara, C.L. Koh, and P. Williams. 2011. Characterization of N-acylhomoserine lactone degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia. BMC Microbiology 11:51. doi:10.1186/1471-2180-11-51.

Chong, T. M., C.L. Koh, C.K. Sam, Y.M. Choo, W.F. Yin, and K.G. Chan. 2012. Characterization of quorum sensing and quorum quenching soil bacteria isolated from Malaysian tropical montane forest. Sensors 12: 4846–4859. doi: 10.3390/s120404846.

Chowdhary, P.K., L. Stewart, C. Lopez, and D.C. Haines. 2008. A single mutation in P450BM-3 enhances acyl homoserine lactone:acyl homoserine substrate binding selectivity nearly 250-fold. J. Biotechnol. 135:374 –376. doi: 10.1016/j.jbiotec.2008.05.005.

Christianto, B., and Y. Yogiara. 2011. Screening of quorum quenching activity of bacteria isolated from ant lion. Microbiology Indonesia 5: 46–49. doi: 10. 5454/mi.5.1.8.

Czajkowski, R., M.C.M. Perombelon, J.A. van Veen, and J.M. van der Wolf. 2011. Control of blackleg and tuber soft rot of potato caused by Pectobacterium and Dickeya species: a review. Plant Pathol. 60: 999–1013. doi: 10.1111/j.1365-3059.2011.02470.x.

Dong, Y.H., J.L. Xu, X.Z. Li, and L.H. Zhang. 2000. AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. P. Natl. Acad. Sci. USA 97: 3526–3531.

Fitriyah, D., A.T. Wahyudi, and I. Rusmana. 2015. Characterization of bacteria producing acyl homoserine lactone (AHL) lactonase from agricultural lands. Adv. Environ. Biol. 9:140-148.

Khoiri, S., T.A. Damayanti, and Giyanto. 2017. Identification of quorum quenching bacteria and its biocontrol potential against soft rot disease bacteria, Dickeya dadantii.AGRIVITA J. Agric. Sci. 39: 45-55.

Kim, A.L., S.Y. Park, C.H. Lee, C.H. Lee, and J.K. Lee. 2014. Quorum quenching bacteria isolated from the sludge of a wastewater treatment plant and their application for controlling biofilm formation. J. Microbiol. Biotechnol. 24: 1574–1582. doi: 10.4014/jmb.1407.07009.

LaSarre, B. and M.J. Federle. 2013. Exploiting quorum sensing to confuse bacterial pathogens. Microbiol. Mol. Biol. Rev. 77: 73-111. doi:10.1128/MMBR.00046-12.

Liu, Q., W. Xiao, Z. Wu, S. Li, Y. Yuan, and H. Li. 2016. Identification of Dickeya dadantii as a causal agent of banana sheat rot in China. J. Plant. Pathol. 98(3):503-510

Ma, A., D. Lv, X. Zhuang, and G. Zhuang. 2013. Quorum quenching in culturable phyllosphere bacteria from tobacco. Int. J. Mol. Sci. 14:14607-14619. doi:10.3390/ijms140714607.

Marchesi, J.R., T. Sato, A.J. Weightman, T.A. Martin, J.C. Fry, S.J. Hiom, D. Dymock, and W.G. Wade. 1998. Design and evaluation of useful bacterium-spesific PCR primer that amplify genes coding for bacterial 16S rRNA. Appl. Environ. Microbiol. 64:795-799.

Molina L., F. Constantinescu, L. Michel, C. Reimmann, B. Duffy, and G. Defago. 2003. Degradation of pathogen quorum-sensing molecules by soil bacteria: A preventive and curative biological control mechanism. FEMS Microbiol. Ecol. 45: 71-81. doi:10.1016/S0168-6496(03)00125-9.

Morohoshi, T., N. Someya, and T. Ikeda. 2009. Novel N-acylhomoserine lactone-degrading bacteria isolated from the leaf surface of Solanum tuberosum and their quorum-quenching properties. Biosci.

Biotechnol. Biochem. 73: 2124–2127. doi: 10.1271/bbb.90283.

Muharam, A., R. Indrasti, and Hanudin. 2012. Occurence of Dickeya dadantii the causal agent of bacterial soft rot on orchid in DKI Jakarta and west Java Indonesia. Crop. Environ. 3:37-44.

Nasser, W., M.L. Bouillant, G. Salmond, and S. Reverchon. 1998. Characterization of the Erwinia chrysanthemi expI–expR locus directing the synthesis of two N‐acyl‐homoserine lactone signal molecules. Mol. Microbiol. 29:1391-1405.

Pan, J., T. Huang, F. Yao, Z. Huang, C.A. Powell, S. Qiu, and X. Guan. 2008. Expression and characterization of aiiA gene from Bacillus subtilis BS-1. Microbiol. Res. 163(6):711- 716. doi:10.1016/j.micres.2007.12.002.

Pustelny, C., A Albers, K. Buldt-Karentzopoulos, K. Parschat, S.R. Chhabra, M. Camara, P. Williams, and S. Fetzner. 2009. Dioxygenase-mediated quenching of quinolone-dependent quorum sensing in Pseudomonas aeruginosa. Chem. Biol. 16: 1259–1267. doi: 10.1016/j.chembiol.2009.11.013.

Romero, M., S.P. Diggle, S. Heeb, M. Camara, and A. Otero. 2008. Quorum quenching activity in Anabaena sp. PCC 7120: Identification of AiiC, a novel AHL-acylase. FEMS Microbiol Lett 280: 73-80. doi: 10.1111/j.1574-6968.2007.01046.x.

Roy, V., R. Fernandes, C.Y. Tsao, and W.E. Bentley. 2010. Cross species quorum quenching using a native AI-2 processing enzyme. ACS Chem. Biol. 5:223–232. doi: 10.1021/cb9002738.

Sakr, M.M., K.M.A. Aboshanab, M.M. Aboulwafa, and N.A.H. Hassouna. 2013. Characterization and complete sequence of lactonase enzyme from Bacillus weihenstephanensis isolate P65 with potential activity against acyl homoserine lactone signal molecules. Biomed. Res. Int. 2013:1-10. doi:10.1155/2013/192589.

Sakr, M.M., M.M. Aboulwafa, K.M.A. Aboshanab, and N.A.H. Hassouna. 2014. Screening and preliminary characterization of quorum quenching acivities of soil Bacillus isolates against acyl homoserine lactose of clinically isolate Pseudomonas aeruginosa. Mal. J. Microbiol. 10:80-91.

Samson, R., J.B. Legendre, R. Christen, M. Fischer-Le Saux, W. Achouak, and L. Gardan. 2005. Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner et al. 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov as Dickeya chrysanthemi comb. nov and Dickeya paradisiaca comb. nov and delineation of four novel species, Dickeya dadantii sp nov., Dickeya dianthicola sp nov., Dickeya dieffenbachiae sp nov and Dickeya zeae sp nov. Int. J. Syst. Evol. Microbiol. 55:1415-1427.

Santoyo, G., Md.C. Orozco-Mosqueda, and M. Govindappa, 2012. Mechanisms of biocontrol and plant growth-promoting activity in soil bacterial species of Bacillus and Pseudomonas: a review. Biocontrol Sci. Technol. 22, 855–872.

Sari, P.E., I. Rusmana, and A. Akhdiya. 2016. AHL-lactonase characteristics of Bacillus thuringiensis SGT3g and its effectiveness in inhibiting pathogenicity of Dickeya dadantii. Malays. J. Microbiol. 12: 315-321.

Song, C., H. Ma, Q. Zhao, S. Song, and Z. Jia. 2012. Inhibition of quorum sensing activity by ethanol extract of Scutellaria baicalensis Georgi. J. Plant Pathol. Microbiol. S7: 001. doi: 10.4172/2157-7471.S7-001.

Vanjildorj. E., S.Y. Song, Z.H. Yang, J.E. Choi, Y.S. Noh, S.Park, W.J. Lim, K.M. Cho, H.D. Yun, Y.P. Lim. 2009. Enhancement of tolerance to soft rot disease in the transgenic Chinese cabbage (Brassica rapa L. ssp. pekinensis) inbred line, Kenshin. Plant Cell. Rep. 28(10):1581-91.

Végh, A., Némethy, Z., Salamon, P., Mándoki, Z., and Palkovics, L. 2014. First report of bacterial wilt on chrysanthemum caused by Dickeya chrysanthemi (syn. Erwinia chrysanthemi) in Hungary. Plant Disease 98(7):988. doi: 10.1094/PDIS-09-13-0948-PDN.

White, C. E., and T.M. Finan. 2009. Quorum quenching in Agrobacterium tumefaciens: Chance or necessity? J. Bacteriol. 191: 1123–1125. doi: 10.1128/JB.01681-08

Yin, X.T., L. Xu, S.S. Fan, L.N. Xu, D.C. Li, and Z.Y. Liu. 2010. Isolation and characterization of an AHL lactonase gene from Bacillus amyloliquefaciens. World J. Microbiol. Biotechnol. 26(8):1361-1367. doi:10.1007/s11274-010-0308-8.

Zhu, W., M.M. Magbanua, and F.F. White. 2000. Identification of two novel hrp-associated genes in the hrp gene cluster of Xanthomonas oryzae pv. oryzae. J. Bacteriol. 182: 1844-1853.

Zou, L., X. Wang,Y. Xiang,B. Zhang,Y. Li,Y. Xiao,J. Wang, A.R. Walmsley, and Gong-you Chen. 2006. Elucidation of the hrp clusters of Xanthomonas oryzae pv. oryzicola that control the hypersensitive response in nonhost tobacco and pathogenicity in susceptible host rice. Appl Environ. Microbiol. 72(9): 62126224.




DOI: http://dx.doi.org/10.21082/jbio.v13n2.2017.p101-110

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Jurnal AgroBiogen

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


         


P-ISSN : 1907-1094
E-ISSN : 2549-1547


Jurnal AgroBiogen

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Jl. Tentara Pelajar 3A, Bogor 16111
Jawa Barat, Indonesia
Telp.: (0251) 8339793, 8337975
Faks.: (0251) 8338820
E-mail: jurnal.agrobiogen@gmail.com
Situs: http://biogen.litbang.pertanian.go.id



View My Stats