Keragaman Jeruk Fungsional Indonesia Berdasarkan Karakter Morfologis dan Marka RAPD

Farida Yulianti, Norry E. Palupi, Dita Agisimanto

Abstract


Identifikasi variabilitas genetik tanaman jeruk diperlukan dalam pengelolaan sumber daya genetik dan proses seleksi dalam
program pemuliaan. Tujuan penelitian adalah mempelajari keragaman lima belas aksesi jeruk fungsional Indonesia
berdasarkan karakter morfologis dan marka molekuler. Penelitian dilaksanakan di Laboratorium Pemuliaan Tanaman, Balai
Penelitian Tanaman Jeruk dan Buah Subtropika, Malang, Jawa Timur. Keragaman morfologis didasarkan pada hasil
pengamatan morfologi, sedangkan keragaman molekuler didasarkan pada hasil amplifikasi marka RAPD. Keragaman
morfologis dan molekuler dianalisis menggunakan program DARWin5. Hasil penelitian menunjukkan bahwa keragaman
morfologis jeruk fungsional Indonesia lebih tinggi dibanding dengan keragaman molekulernya. Keragaman tertinggi
berdasarkan karakter morfologis terjadi antara Citrumelo dan Carrizo Citrange dengan Lemon seedless (79%), sedangkan
keragaman tertinggi berdasarkan marka RAPD terjadi antara Lemo Swangi dan Lemon seedless (49%). Pita DNA spesifik
ditemukan oleh marka OPH15(235,280) pada aksesi Jari Budha, Etrog Citron, dan Lemon seedless, marka OPD07(400) dan
OPC17(416) pada aksesi dengan daun tipe trifoliata (Troyer Citrange, Citrumelo, dan Carrizo Citrange) dan OPH04(583) dan
OPD07(330) pada aksesi Keprok Tening.


Keywords


Keragaman morfologis; keragaman molekuler; jeruk fungsional; RAPD.

Full Text:

PDF

References


Agisimanto, D., H. Arisah, dan N.E. Palupi. 2015.

Pengembangan protokol produksi limonin dan naringin

dari jeruk dan penggunaan ekstraknya dalam partikel

nano. Balai Penelitian Tanaman Jeruk dan Buah

Subtropika, Malang.

Amar, M.H., M.K. Biswas, Z.W. Zhang, and W.W. Guo.

Exploitation of SSR, SRAP, and CAPS-SNP

markers for genetic diversity of Citrus germplasm

collection. Sci. Hort. 128:220–227.

Baig, M.N.R., S. Grewal, and S. Dhillon. 2009. Molecular

characterization and genetic diversity analysis of citrus

cultivars by RAPD markers. Turk. J. Agric. For.

:375–384.

Biswas, M.K., L. Chai, M.H. Amar, X. Zhang, and X.X.

Deng. 2011. Comparative analysis of genetic diversity

in Citrus germplasm collection using AFLP, SSAP,

SAMPL, and SSR markers. Sci. Hort. 129:798–803.

Coletta-Filho, H.D., M.A. Machado, M.L.P.N. Targon,

M.C.P.Q.D.G. Moreira, and J. Pompeu Jr. 1998.

Analysis of the genetic diversity among mandarin

(Citrus sp.) using RAPD markers. Euphytica 102:133–

Corazza-Nunes, M.J., M.A. Machado, W.M.C. Nunes, M.

Cristofani, and M.L.P.N. Targon. 2002. Assessment of

genetic variability in grapefruits (Citrus paradisi Macf.)

and pummelos (C. maxima [Burm.] Merr.) using RAPD

and SSR markers. Euphytica 126:169–176.

Doyle, J.J. and J.L. Doyle. 1990. Isolation of plant DNA

from fresh tissue. Focus 12:13–15.

Gorinstein, S., O. Martı́n-Belloso, P. Yong-Seo, R.

Haruenkit, A. Lojek, M. Ĉı́ž, A. Caspi, I. Libman, and S.

Trakhtenberg. 2001. Comparison of some biochemical

characteristics of different citrus fruits. Food Chem.

:309–315.

Hasler, C.M. 2002. Functional foods: Benefits, concerns,

and challenges. A position paper from the American

Council on Science and Health. J. Nutr. 132:3772–

Hussein, E.H.A., S.M.M. Abd-alla, N.A. Awad, and M.S.

Hussein. 2004. Assessment of genetic variability and

genotyping of some citrus accessions using molecular

markers. Arab J. Biotech. 7(1):23–36.

International Life Sciences Institute. 1999. Functional food.

Crit. Rev. Food Sci. Nutr. 39:203–316.

International Plant Genetic Resources Institute. 1999.

Descriptor for citrus. International Plant Genetic

Resources Institute, Rome, Italy.

Koehler-Santos, P., A.L.C. Dornelles, and L.B.D. Freitas.

Characterization of mandarin citrus germplasm

from Southern Brazil by morphological and molecular

analyses. Pesq. Agropec. Bras. 38:797–806.

Ling, P., L.W. Duncan, Z. Deng, D. Dunn, X. Hu, S. Huang,

and F.G. Gmitter. 2000. Inheritance of citrus nematode

resistance and its linkage with molecular markers.

Theor. Appl. Genet. 100(7):1010–1017.

Malik, S.K., M.R. Rohini, S. Kumar, R. Choudhary, D. Pal,

and R. Chaudhury. 2012. Assessment of genetic

diversity in sweet orange (Citrus sinensis [L.] Osbeck)

cultivars of India using morphological and RAPD

markers. Agric. Res. 1(4):317–324.

Mestre, P.F., M.J. Asins, E.A. Carbonell, and L. Navarro.

New gene(s) involved in the resistance of

Poncirus trifoliata (L.) Raf. to Citrus tristeza virus.

Theor. Appl. Genet. 95:691–695.

Moore, G.A. 2001. Oranges and lemons, clues to the

taxonomy of from molecular markers. Trends Genet.

:536–540.

Penjor, T., T. Mimura, R. Matsumoto, M. Yamamoto, and Y.

Nagano. 2014. Characterization of limes (Citrus

aurantifolia) grown in Bhutan and Indonesia using

high-throughput sequencing. Sci. Rep. 4:4853.

doi:10.1038/srep04853.

Pessina, D., R. Gentili, G. Barcaccia, S. Nicolè, G. Rossi, S.

Barbesti, and S. Sgorbati. 2011. DNA content,

morphometric and molecular marker analyses of Citrus

limonimedica, C. limon, and C. medica for the

determination of their variability and genetic

relationships within the genus Citrus. Sci. Hort.

:663–673.

Rao, M.N., J.R. Soneji, C. Chen, S. Huang, and F.G.

Gmitter. 2008. Characterization of zygotic and nucellar

seedlings from sour orange-like citrus rootstock

candidates using RAPD and EST-SSR markers. Tree

Genet. Genomes 4:113–124.

Ruiz, C., M.P. Breto, and M.J. Asins. 2000. A quick

methodology to identify sexual seedlings in citrus

breeding programs using SSR markers. Euphytica

:89–94.

Siragusa, M., F.D. Pasquale, L. Abbate, L. Martorana, and

N. Tusa. 2008. The genetic variability of Sicilian lemon

germplasm revealed by molecular marker fingerprints.

J. Am. Soc. Hort. Sci. 133:242–248.

Weising, K., H. Nybom, K. Wolff, and G. Kahl. 2005. DNA

fingerprinting in plants: Principles, methods, and

applications. CRC Press. Boca Raton, FL, USA.

Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski, and

S.V. Tingey. 1990. DNA polymorphisms amplified by

arbitrary primers are useful as genetic markers.

Nucleic Acids Res. 18:6531–6535.

Yoon, H.Y., S.I. Yun, B.Y. Kim, Q. Jin, E.R. Woo, S.Y.

Jeong, and Y.S. Chung. 2011. Poncirin promotes

osteoblast differentiation but inhibits adipocyte

differentiation in mesenchymal stem cells. Eur. J.

Pharmacol. 664(1–3):54–59.

Yuan-Chuen, W., C. Yueh-Chueh, and K. Yu-Hua. 2007.

Quantitation of bioactive compounds in citrus fruits

cultivated in Taiwan. Food Chem. 102:1163−1171




DOI: http://dx.doi.org/10.21082/jbio.v12n2.2016.p91-100

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2018 Jurnal AgroBiogen

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

           

P-ISSN : 1907-1094
E-ISSN : 2549-1547

Jurnal AgroBiogen

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Jl. Tentara Pelajar 3A, Bogor 16111
Jawa Barat, Indonesia
Telp.: (0251) 8339793, 8337975
Faks.: (0251) 8338820
E-mail: jurnal.agrobiogen@gmail.com
Situs: http://biogen.litbang.pertanian.go.id


View My Stats