Pengaruh Iradiasi Sinar Gama terhadap Pertumbuhan dan Regenerasi Kalus Padi Varietas Ciherang dan Inpari 13

Rossa Yunita, Nurul Khumaida, Didy Sopandie, Ika Mariska

Abstract


Effect of gamma irradiation on rice callus depends
on the irradiation dose used. Irradiation dose is one of the
factors that affect the genetic changes in the cells of plants.
High doses can result in tissue death, meanwhile low doses
will result in abnormal changes in plant phenotype. The level
of sensitivity of a plant tissue against gamma irradiation is
different. This study aimed to evaluate the growth and
regeneration of callus in various irradiation doses of gamma
ray. The plant materials used were mature embryos of rice
var. Ciherang and Inpari 13. This study used a completely
randomized design with gamma irradiation treatment at
doses of 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 Gy. Each
treatment consisted of 10 replicates with 5 embryogenic calli
per bottle. The results showed that the increasing doses of
gamma irradiation increased the percentage of dead callus,
inhibited callus growth, and its ability to regenerate. The
high percentages of callus of Ciherang and Inpari 13 that
formed green spots and adventitious shoots were mostly
obtained from controls. The percentages decreased at
irradiation doses of 10, 20, 30, and 40 Gy. Moreover, the calli
of Ciherang and Inpari 13 were not able to form adventitious
shoots at irradiation doses higher than 40 Gy and 30 Gy,
respectively.


Keywords


Irradiation dose; lethal dose irradiation; rice callus; gamma ray.

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DOI: http://dx.doi.org/10.21082/jbio.v10n3.2014.p101-108

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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