Survei Polimorfisme Tetua untuk Pengembangan Panel CSSL Padi (Oryza sativa L.) dan Identifikasi Tanaman F1

Mariana Susilowati, Panjisakti Basunanda, Wening Enggraini, Ma'sumah Ma'sumah, Kurniawan R. Trijatmiko

Abstract


Raising yield potential of modern
indica varieties is essential to meet the increased demand of
rice production. This is due to increased human population,
threats of climate change and degradation of agricultural
resources. The use of chromosome segment substitution
lines (CSSL) is more effective for identification of genes
those are useful for improvement of yield potential. The aims
of this study were to observe the morphological trait
differences between recipient parent (var. Ciherang) and
three candidates of donor parent (var. Fatmawati and new
plant type lines, i.e. B12743 and B11143D), to identify
polymorphic SSR markers among them and to verify F1
individuals. Ciherang and B11143D showed significant
differences on flowering time, plant height, flag leaf area,
tiller number, productive tiller number, panicle length,
spikelet number per panicle and 1,000 grain weight. The rate
of SSR marker polymorphisms between Ciherang and
B11143D was the highest, where 155 of 513 markers (30.2%)
were polymorphic. Marker genotyping using three
polymorphic markers showed that 26 of 27 plants resulted
from the cross of Ciherang х B11143D were F1. These F1
plants could become the basis of CSSL panel that facilitate
the mapping of genes responsible for increasing the yield
potential.


Keywords


CSSL; Ciherang; new plant type; Oryza sativa; SSR markers.

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DOI: http://dx.doi.org/10.21082/jbio.v10n3.2014.p85-92

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