Konstitusi Genetik dan Karakter Fenotipik Galur-galur Padi Pup1 Turunan Varietas Situ Bagendit

Suwaji Handaru Wardoyo, Miftahudin Miftahudin, Sugiono Moeljopawiro, Joko Prasetiyono

Abstract


Acidity, phosphorus deficiency, and drought
stress are major problems in Indonesia’s Ultisol rice farming.
Development of rice lines tolerant to those stresses is
expected to be able to reduce the consumption of P
fertilizer. The objectives of the research were to evaluate
genetic constitutive of rice lines (BC2F6 population) derived
from Situ Bagendit x Kasalath and Situ Bagendit x NIL-C433
crossings, and to evaluate responses of those lines to
Yoshida nutrient solution under P deficiency and Al stress
condition. The research was conducted at Molecular Biology
Laboratory and Greenhouse of ICABIOGRAD, from
November 2011 to May 2013. The result of foreground
analysis showed that Pup1 locus has been integrated into
the genome of BC2F6 rice lines, eventhough some lines (SK5,
SK6, SK7, SK8, SK9, SK10, SK19, and SK20) showed
incomplete integration. Background analysis indicated that
majority (95.7%) of the Situ Bagendit background has been
recovered in BC2F6 rice lines. Al stress evaluation showed SN
lines were more tolerant to P deficiency and Al stress than
that of SK lines. Pup1 locus showed good expression under
low P and no Al stress. Based on genome proportion and
Yoshida nutrient solution experiments, a total of three lines,
namely SK13, SN2, and SN9, have potential good
characteristics. Molecular analysis within a marker-assisted
backcrossing (MAB) experiment should be carried out at
each generation of lines for gaining fully gene segment in
advanced generations.


Keywords


Rice; Pup1; foreground; background; Yoshida nutrient solution.

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DOI: http://dx.doi.org/10.21082/jbio.v10n2.2014.p61-68

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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