Evaluasi Lapang dan Identifikasi Molekuler Plasma Nutfah Padi terhadap Keracunan Fe

Dwinita W. Utami, Ida Hanarida

Abstract


Fe toxicity is one of the abiotic constraints that
can significantly decrease rice production, especially in
marginal wetlands. The use of tolerant varieties can reduce
the cost of soil processing and fertilizing. Many accessions of
rice germplasm have potential alleles that can be utilized to
create new varieties tolerant to Fe toxicity. The objectives of
this research were to evaluate the Fe toxicity tolerance of
rice germplasm and to analyze the genotype diversity using
SNP markers for OsIRT, Fe toxicity tolerant gene(s). Fe
toxicity tolerant rice germplasms were screened in acid
marginal wetlands of Taman Bogo Experimental Station,
Indonesian Soil Research Insitute, Lampung Province.
Meanwhile, the genotypes performance analysis was
conducted on SNP genotyping analysis using SNP markers
for OsIRT gene(s). Based on phenotypic data of 97
accessions, which were clustered into six groups, two of
them (group 2 and group 5) consisted of the tolerant
accessions at both vegetative and generative stages. The
results of grouping analysis of genotyping based on SNP
markers were obtained that there were five genotype
groups: AGT, AAT, GAT, AAC, and GAC. The AGT genotype
cluster was dominated by the accessions included in group
1. Meanwhile, the GAT genotype cluster consisted of mixed
tolerant and untolerant accessions to Fe toxicity. The GAC
genotype cluster was dominated by the accessions included
in group 2. The accessions which were included in the best
tolerant group, group 5, were separated in different
genotype cluster. Based on association analysis, among the
three SNP markers, OsIRT1 was the most significant SNP
marker (P value = 0.01) which correlated to Fe toxicity
tolerant on vegetative stage. Some of the selected
accessions that were tolerant to Fe toxicity and had good
agronomic performance on acid soil with high Fe content
were Ketan Alay, Markuti, Arias Halus, Komas a, Lantiak,
and Utri Deli. These local rice accessions have the potential
alleles of OsIRT genes.


Keywords


Rice germplasm; phenotype; genotype; Fe toxicity; OsIRT.

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DOI: http://dx.doi.org/10.21082/jbio.v10n1.2014.p9-17

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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