TINJAUAN Penggunaan Suspensi Sel dalam Kultur In Vitro

Sri Hutami

Abstract


Cell suspension culture could be defined as a
process that allows rapidly dividing homogenous suspension
of cells to grow in liquid nutrient media. There are two main
types of suspension cultures: (1) Batch cultures in which
cells are nurtured in a fixed volume of medium until growth
ceases and (2) Continuous cultures in which cell growth is
maintained by continuous replenishment of sterile nutrient
media. Plant cell suspension cultures are mostly used for the
biochemical investigation of cell physiology, growth, metabolism,
protoplast fusion, transformation and for large scale
production of seed by bioreactor and production of secondary
metabolites. Contamination is one of the largest problems
when dealing with cell cultures. Differences between
the products of cell suspension culture and whole plant are
frequently observed. These phenomena’s may be resulted
from lack of differentiation and organization and cell cultureinduced
variation. Utilization of cell suspension culture in
Indonesia is still limited, some of them for mass production
of plantation seed with bioreactor system and for production
of secondary metabolites. The success of this study give the
opportunity for mass production of seeds from other plants
and also production of secondary metabolites.


Keywords


Cell suspension culture; seed production; secondary metabolites.

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DOI: http://dx.doi.org/10.21082/jbio.v5n2.2009.p84-92

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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