Analisis Keragaman Genetik Acremonium yang Berasosiasi dengan Tanaman Gaharu Menggunakan Teknik Random Amplified Polymorphic DNA (RAPD)

Dewi Rahmawati, Nurita Toruan-Mathius

Abstract


Agarwood
or gaharu is a plant that has a high economic value in Asia,
due to its use for production of incense and traditional
medicines. The agarwood formation occurs in the trunk and
roots of trees that have been infected by a fungus, such as
Acremonium spp. Various fungi were associated with the
agarwood formation. Acremonium is generally considered as
highly polyphyletic, contains distantly related fungi. A study
was done to identify genetic diversities in 10 isolates of
Acremonium spp. from four different areas in Indonesia that
are associated with Aquilaria and Gyrinops verstigii using the
Random Amplified Polymorphic DNA (RAPD) technique.
Eight RAPD primers, i.e., OPA 02, OPB 04, OPB 07, OPB 17,
OPC 11, OPD 03, OPD 05, and OPE 07 were used in the
analyses. The results indicated that similarity index values of
the genetic variation ranged from 0.21 to 0.97. Based on the
Nei and Li’s similarity coefficients, these values indicating
the presence of high degree of genetic variability. The lowest
degree of genetic similarity were found between isolates F
(Acremonium spp., which is associated with G. verstigii from
Mataram, Nusa Tenggara Barat), and LM2 from south coastal
area of West Sumatra. The highest genetic similarity value
(0.97) was found between isolates Sr2 and Sr4 from Sorong,
Papua. Results from the cluster analysis indicated that the
isolates could be grouped into two major clusters that were
associated with their geographical locations.


Keywords


Agarwood; genetic diversity; Acremonium spp.; RAPD.

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DOI: http://dx.doi.org/10.21082/jbio.v5n2.2009.p65-70

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