Pengembangan Populasi Mutan Penanda Aktivasi: I. Transformasi Padi Japonica Tropis Lokal Sulawesi cv. Asemandi dengan bantuan Agrobacterium tumefaciens

Atmitri Sisharmini, Aniversari Apriana, Wening Enggraini, Kurniawan R. Trijatmiko

Abstract


The rice transformation technology
is not only provides valuable methods for the introduction
of useful genes into rice plant to improve important
agronomic traits, but also helps in studying gene function
and regulation based on rice genome sequence information.
Knockout of genes by insertional mutagenesis is a straightforward
method to identify gene functions. One of the
methods to develop rice mutants is through genetic transformation
mediated by Agrobacterium using activation
tagging by Ac-Ds system. A study was done with an objective
to obtain mutant rice of local tropical japonica cv. Asemandi
through genetic trans-formation mediated by Agrobacterium
tumefaciens. The transformation was conducted using
Agrobacterium vector with the strain of Agl-1 containing
activation tag construct. The result of experiment showed
that it has been obtained 17 independent line (304 plants)
transgenic Asemandi containing activation tag construct.
These starter lines will be used as materials to develop
several generations of stabil rice mutant through selfing.


Keywords


Genetic transformation, rice cv. Asemandi; Agrobacterium tumefaciens; activation tagging.

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DOI: http://dx.doi.org/10.21082/jbio.v5n2.2009.p49-56

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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