Analisis Keragaman Genetik 161 Aksesi Mangga Indonesia Menggunakan Marka Mikrosatelit

Abstract

Mango is one of the five
important fruit crops in the world. Microsatellite markers can
be used to analyze genetic diversity among mango
accessions. The purpose of this research was to determine
the relationship among mango germplasm collection using
microsatellite markers. A total of 161 mango accessions
originated from Indonesian Tropical Fruit Research Institute
(Cukurgondang Field Station), Pasuruan, East Java, were
used in this research. Twenty-six microsatellite markers
were used to genotype each accession. Genotyping was
conducted using Beckman Coulter® CEQ™ 8000 machine.
Genetic relationship among accecions was analyzed using
the Unweighted Pair Group Method with Arithmetic Mean
(UPGMA) method, followed by bootstrap analysis. The result
showed that high allele variation (15-75 alleles) was
observed among mango accesions tested, with an average
allele number of 38.69. The average of Polymophism
Information Content (PIC) value was 0.548 (0.021-0.949).
Fifteen microsatellite markers showed PIC value >0.5
indicated that these markers were suitable for mango
diversity studies. Cluster analysis divided the mango
collections into two groups. Group I consisted of 95
accessions, and group II consisted of 66 accessions. Ninety
Indonesian indigenous mangos (84.11% of Indonesian
mango accessions) could be separated from the introduced
accessions.